Detection of feline immunodeficiency provirus by seminested polymerase chain reaction

2000 ◽  
Vol 45 (2) ◽  
pp. 161-165 ◽  
Author(s):  
V. Celer ◽  
H. Kulhánková ◽  
V. Celer
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Seminested Polymerase Chain Reaction

Diagnosis of intra-oral MALT lymphoma using seminested polymerase chain reaction

2004 ◽  
Vol 42 (1) ◽  
pp. 28-32 ◽  
Author(s):  
Kazufumi Honda ◽  
Hiroshi Kusama ◽  
Satoshi Takagi ◽  
Shigeki Sekine ◽  
Masayuki Noguchi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Malt Lymphoma ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Seminested Polymerase Chain Reaction

Seminested Polymerase Chain Reaction (PCR) for Detecting Helicobacter pylori DNA in Carotid Atheromas

2006 ◽  
Vol 15 (3) ◽  
pp. 174-179 ◽  
Author(s):  
Eugenia Arias ◽  
Horacio Martinetto ◽  
Marcelo Schultz ◽  
Sebastian Ameriso ◽  
Santiago Rivera ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Seminested Polymerase Chain Reaction

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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