Polyclonal immunoglobulin response of thymic, hepatic and splenic lymphocytes from fetal, germ-free and conventionally reared pigs to different B-cell activators

1995 ◽  
Vol 40 (4) ◽  
pp. 421-430 ◽  
Author(s):  
B. Cukrowska ◽  
J. Šinkora ◽  
Z. Řeháková ◽  
I. Šplíchal ◽  
L. Tučková ◽  
...  
Keyword(s):  
B Cell ◽  
Splenic Lymphocytes ◽  
Germ Free ◽  
Polyclonal Immunoglobulin ◽  
Immunoglobulin Response

scholarly journals “An Intrinsic Program Determines Key Age-Associated Changes in Adaptive Immunity That Limit Response to Non-Pathogens”

2021 ◽  
Vol 2 ◽  
Author(s):  
Susan L. Swain ◽  
Olivia Kugler-Umana ◽  
Susan L. Tonkonogy
Keyword(s):  
B Cell ◽  
Adaptive Immune System ◽  
Housing Conditions ◽  
Germ Free ◽  
Cell Responses ◽  
Adaptive Immune ◽  
B Cell Subsets ◽  
Poor Efficacy ◽  
Normal Laboratory ◽  
Intrinsic Program

As mice age their adaptive immune system changes dramatically, leading to weakened responses to newly encountered antigens and poor efficacy of vaccines. A shared pattern emerges in the aged, with both CD4 T and B cell responses requiring higher levels of pathogen recognition. Moreover, in aged germ-free mice we find accumulation of the same novel age-associated T and B cell subsets that we and others have previously identified using mice maintained in normal laboratory animal housing conditions, suggesting that their development follows an intrinsic program.

scholarly journals Etiologic aspects of cold agglutinin disease: evidence for cytogenetically defined clones of lymphoid cells and the demonstration that an anti-Pr cold autoantibody is derived from a chromosomally aberrant B cell clone

Blood ◽  
1986 ◽  
Vol 67 (6) ◽  
pp. 1705-1709 ◽  
Author(s):  
LE Silberstein ◽  
GA Robertson ◽  
AC Harris ◽  
L Moreau ◽  
E Besa ◽  
...  
Keyword(s):  
B Cell ◽  
Peripheral Blood ◽  
Cell Clone ◽  
Peripheral Blood Lymphocytes ◽  
Lymphoid Cells ◽  
Cold Agglutinin ◽  
Additional Patient ◽  
Splenic Lymphocytes ◽  
Cold Agglutinin Disease ◽  
Karyotypic Analysis

Abstract This study investigated the clonal nature of cold agglutinin disease in a series of nine patients, which included the benign or idiopathic form as well as cases with an underlying lymphoma. Surface marker phenotyping and karyotypic analysis were performed on peripheral blood lymphocytes. An increased proportion of B cells was found in four cases and in three of these patients a monoclonal B cell population was identified with a mu, kappa phenotype. In the same three cases, as well as an additional patient, an aberrant karyotype was demonstrated. The cytogenetic abnormality present in all four cases included trisomy 3; two patients also had a trisomy 12. One of these four patients had a well-differentiated lymphoma and underwent a splenectomy. Splenic lymphocytes were transformed with Epstein-Barr virus and cultured en masse. Eight clones were established producing the same cold agglutinin with identical specificity as that present in the patient's plasma. Five of these clones were studied cytogenetically, and all had the same abnormal karyotype (51,XX,+3,+9,+12,+13,+18) found in peripheral blood and splenic lymphocytes. Thus, in this case, the cold reactive autoantibody was produced by the chromosomally abnormal, neoplastic clone of lymphocytes. Our findings support the view that cold agglutinin disease represents a spectrum of clonal disorders.

scholarly journals The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2

2014 ◽  
Vol 45 (1) ◽  
Author(s):  
Marek Sinkora ◽  
John E Butler ◽  
Kelly M Lager ◽  
Hana Potockova ◽  
Jana Sinkorova
Keyword(s):  
B Cell ◽  
Lymphoid Cells ◽  
Germ Free

scholarly journals Antigenic Stimuli do not Influence Thymic B Lymphocytes: A Morphological and Functional Study in Germ-Free and Conventionally Reared Piglets

1998 ◽  
Vol 6 (3-4) ◽  
pp. 171-178 ◽  
Author(s):  
B. Cukrowska ◽  
I. Trebichavský ◽  
P. Rossmann ◽  
Z. Reháková ◽  
J. ŠInkora ◽  
...  
Keyword(s):  
B Cell ◽  
B Lymphocytes ◽  
Cell Subpopulation ◽  
Functional Study ◽  
Germ Free ◽  
Food Antigens ◽  
Before And After ◽  
Embryonic Life ◽  
Thymic Medulla ◽  
Immunohistological Analysis

We have recently reported that thymic B lymphocytes (TBL) are the first B-cell subpopulation undergoing isotype switching to IgG and IgA during embryonic life. The aim of this study is to analyze the influence of antigenic stimulation on TBL location and activity using a germ-free (GF) newborn pig model, in which maternal antibodies and antigens do not affect B-cell development. Immunohistological analysis showed that TBL were disseminated mainly in the thymic medulla. There were no differences in the distribution of TBL, both in GF newborn piglets before and after colonization withEscherichia coliand in older conventionally reared (CONV) piglets. The number of immunoglobulin (Ig)-secreting cells measured by the ELISPOT method was not influenced by microflora and food antigens. IgM-positive cells secreting IgM and CD45RC-positive cells spontaneously producing IgM, IgG, and IgA were detected in newborn thymus.Our findings suggest that TBL differentiation and Ig switching to IgG and IgA-secreting cells is not influenced by external antigens and that the thymic microenviroment plays an important role in this process.

scholarly journals Etiologic aspects of cold agglutinin disease: evidence for cytogenetically defined clones of lymphoid cells and the demonstration that an anti-Pr cold autoantibody is derived from a chromosomally aberrant B cell clone

Blood ◽  
1986 ◽  
Vol 67 (6) ◽  
pp. 1705-1709
Author(s):  
LE Silberstein ◽  
GA Robertson ◽  
AC Harris ◽  
L Moreau ◽  
E Besa ◽  
...  
Keyword(s):  
B Cell ◽  
Peripheral Blood ◽  
Cell Clone ◽  
Peripheral Blood Lymphocytes ◽  
Lymphoid Cells ◽  
Cold Agglutinin ◽  
Additional Patient ◽  
Splenic Lymphocytes ◽  
Cold Agglutinin Disease ◽  
Karyotypic Analysis

This study investigated the clonal nature of cold agglutinin disease in a series of nine patients, which included the benign or idiopathic form as well as cases with an underlying lymphoma. Surface marker phenotyping and karyotypic analysis were performed on peripheral blood lymphocytes. An increased proportion of B cells was found in four cases and in three of these patients a monoclonal B cell population was identified with a mu, kappa phenotype. In the same three cases, as well as an additional patient, an aberrant karyotype was demonstrated. The cytogenetic abnormality present in all four cases included trisomy 3; two patients also had a trisomy 12. One of these four patients had a well-differentiated lymphoma and underwent a splenectomy. Splenic lymphocytes were transformed with Epstein-Barr virus and cultured en masse. Eight clones were established producing the same cold agglutinin with identical specificity as that present in the patient's plasma. Five of these clones were studied cytogenetically, and all had the same abnormal karyotype (51,XX,+3,+9,+12,+13,+18) found in peripheral blood and splenic lymphocytes. Thus, in this case, the cold reactive autoantibody was produced by the chromosomally abnormal, neoplastic clone of lymphocytes. Our findings support the view that cold agglutinin disease represents a spectrum of clonal disorders.

scholarly journals Dysfunction of B Cell Leading to Failure of Immunoglobulin Response Is Ameliorated by Dietary Silk Peptide in 14-Month-Old C57BL/6 Mice

2020 ◽  
Vol 7 ◽  
Author(s):  
Sungwoo Chei ◽  
Hyun-Ji Oh ◽  
Kippeum Lee ◽  
Heegu Jin ◽  
Jeong-Yong Lee ◽  
...  
Keyword(s):  
B Cell ◽  
Silk Peptide ◽  
Immunoglobulin Response
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