Isolation and characterization of a serially cultivated, neoplastic, epithelial cell line from theN-nitrosomethylurea induced rat mammary adenocarcinoma

In Vitro ◽  
1982 ◽  
Vol 18 (6) ◽  
pp. 565-575 ◽  
Author(s):  
Leonard A. Cohen
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Mammary Adenocarcinoma ◽  
Epithelial Cell Line ◽  
Isolation And Characterization ◽  
Neoplastic Epithelial Cell

scholarly journals Isolation and characterization of a neoplastic epithelial cell line derived from irradiated human submaxillary gland

1979 ◽  
Vol 25 (3) ◽  
pp. 559-564
Author(s):  
Kanemitsu SHIRASUNA ◽  
Mitsunobu SATO ◽  
Yoshiaki YURA ◽  
Tetuo YANAGAWA ◽  
Kazuko KUBO ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Submaxillary Gland ◽  
Epithelial Cell Line ◽  
Isolation And Characterization ◽  
Neoplastic Epithelial Cell

Isolation and characterization of a breast progenitor epithelial cell line with robust DNA damage responses

2006 ◽  
Vol 98 (3) ◽  
pp. 357-364 ◽  
Author(s):  
Kate C. Shen ◽  
Fred Miller ◽  
Larry Tait ◽  
Steven J. Santner ◽  
Robert Pauley ◽  
...  
Keyword(s):  
Dna Damage ◽  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Isolation And Characterization ◽  
Dna Damage Responses

Regulation of the human Na+-glucose cotransporter gene, SGLT1, by HNF-1 and Sp1

2000 ◽  
Vol 278 (4) ◽  
pp. G591-G603 ◽  
Author(s):  
Martín G. Martín ◽  
Jiafang Wang ◽  
R. Sergio Solorzano-Vargas ◽  
Jason T. Lam ◽  
Eric Turk ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Critical Role ◽  
Chinese Hamster ◽  
Intestinal Cell ◽  
Epithelial Cell Line ◽  
Strong Activity ◽  
Isolation And Characterization ◽  
Gc Box

The Na+-glucose cotransporter ( SGLT1) is expressed primarily by small intestinal epithelial cells and transports the monosaccharides glucose and galactose across the apical membrane. Here we describe the isolation and characterization of 5.3 kb of the 5′-flanking region of the SGLT1 gene by transiently transfecting reporter constructs into a variety of epithelial cell lines. A fragment (nt −235 to +22) of the promoter showed strong activity in the intestinal cell line Caco-2 but was inactive in a nonintestinal epithelial cell line (Chinese hamster ovary). Within this region, three cis-elements, a hepatocyte nuclear factor-1 (HNF-1) and two GC box sites are critical for maintaining the gene's basal level of expression. The two GC boxes bind to several members of the Sp1 family of transcription factors and, in the presence of HNF-1, synergistically upregulate transactivation of the promoter. A novel 16-bp element just downstream of one GC box was also shown to influence the interaction of Sp1 to its binding site. In summary, we report the identification and characterization of the human SGLT1 minimal promoter and the critical role that HNF-1 and Sp1-multigene members have in enhancing the basal level of its transcription in Caco-2 cells.

Isolation and Characterization of a New Human Corneal Epithelial Cell Line: HCE-F

Cornea ◽  
2020 ◽  
Vol 39 (11) ◽  
pp. 1419-1425
Author(s):  
Martina Cristaldi ◽  
Melania Olivieri ◽  
Giorgia Spampinato ◽  
Carmelina Daniela Anfuso ◽  
Marina Scalia ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Corneal Epithelial Cell ◽  
Epithelial Cell Line ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cell ◽  
Isolation And Characterization
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