Studies of blood cholinesterase activity in liver desease (2nd report) Change of esterase zymogram by thin layer agar gel electrophoresis

1967 ◽  
Vol 2 (2) ◽  
pp. 164-165
Author(s):  
H. Saito ◽  
Y. Sugawara ◽  
K. Komatubara ◽  
T. Niimi ◽  
T. Adachi ◽  
...  
Keyword(s):  
Thin Layer ◽  
Gel Electrophoresis ◽  
Cholinesterase Activity ◽  
Agar Gel ◽  
Agar Gel Electrophoresis

POLYETHYLENE GLYCOL ON AGAR-GEL ELECTROPHORESIS

The Lancet ◽  
1974 ◽  
Vol 304 (7892) ◽  
pp. 1321-1322
Author(s):  
W.H. Taylor ◽  
D.J. Etherington
Keyword(s):  
Polyethylene Glycol ◽  
Gel Electrophoresis ◽  
Agar Gel ◽  
Agar Gel Electrophoresis

scholarly journals Hemoglobin Hope: A Beta Chain Variant

Blood ◽  
1965 ◽  
Vol 25 (5) ◽  
pp. 830-838 ◽  
Author(s):  
VIRGINIA MINNICH ◽  
ROBERT J. HILL ◽  
PHILIP D. KHURI ◽  
MARY E. ANDERSON
Keyword(s):  
Blood Cells ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Beta Chain ◽  
Agar Gel ◽  
Hemoglobin A ◽  
Abnormal Hemoglobin ◽  
Starch Gel ◽  
Agar Gel Electrophoresis ◽  
Chain Variant

Abstract A new hemoglobin, hemoglobin Hope, with a beta chain abnormality has been found in three generations of a St. Louis Negro family. The abnormal hemoglobin in the heterozygous state caused neither clinical stigmata nor abnormality in the red blood cells. Hemoglobin Hope was detected by agar gel electrophoresis at pH 6.2, but could not be differentiated from hemoglobin A by starch block electrophoresis at pH 8.6. Also, it could not be separated from hemoglobin A by paper, or starch gel electrophoresis employing a range of buffers from pH 6.2 to 8.6. Amino acid analysis showed that aspartic acid was substituted for glycine at position 136 of the beta chain. Hemoglobin Hope may be formulated as α2Aβ2136 gly-asp.

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