Nylon tube-immobilized creatinine iminohydrolase and glutamate dehydrogenase in serum and urine creatinine analysis

1984 ◽  
Vol 9 (3) ◽  
pp. 217-230
Author(s):  
Janet S. Colliss ◽  
Ron Ginman
Keyword(s):  
Glutamate Dehydrogenase ◽  
Urine Creatinine ◽  
Nylon Tube ◽  
Serum And Urine

Estimation of Creatinine by the Jaffe Reaction

1968 ◽  
Vol 14 (3) ◽  
pp. 222-238 ◽  
Author(s):  
Harry Husdan ◽  
Abraham Rapoport
Keyword(s):  
Color Development ◽  
Factors Influencing ◽  
Urine Creatinine ◽  
Sample Stability ◽  
Serum And Urine

Abstract Total chromogen, true, and AutoAnalyzer methods of measuring serum and urine creatinine by the Jaffe reaction were investigated. Some factors influencing this reaction were examined. These included wavelength, blank, linearity, and conditions of color development. Modifications of the three methods were made and their precision, recovery, and sample stability determined. The interference of ketones and glucose were measured. Finally, the values obtained by the three methods on the same samples of serum and urine were compared statistically.

Rise in serum and urine creatinine after phenacemide

1978 ◽  
Vol 23 (4) ◽  
pp. 430-437 ◽  
Author(s):  
Richard K. Richards ◽  
Thorir D. Bjornsson ◽  
L. David Waterbury
Keyword(s):  
Urine Creatinine ◽  
Serum And Urine

Enzymatic rate assay of creatinine in serum and urine

1993 ◽  
Vol 39 (10) ◽  
pp. 2130-2136 ◽  
Author(s):  
T Fujita ◽  
S Takata ◽  
Y Sunahara
Keyword(s):  
Glutamate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Second Step ◽  
Tetraacetic Acid ◽  
Trigger System ◽  
Step Method ◽  
Metal Chelating ◽  
Chelating Reagent ◽  
Serum And Urine ◽  
Serum And Urine Samples

Abstract A two-step method for assaying creatinine in serum and urine samples, suitable with automated analyzers, is reported. Reagent 1, for the first step, contains a blanking system [creatine amidinohydrolase (CRTase), urease, glutamate dehydrogenase, NADPH, and 2-oxoglutarate] and a NADPH-regenerating system [Mg(2+)-dependent isocitrate dehydrogenase (ICD), MgCl2, and excess isocitrate]. Reagent 2, for the second step, contains the metal-chelating reagent trans-1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid (CyDTA) and a trigger system [creatinine amidohydrolase (CRNase)]. When a specimen is mixed with reagent 1, all the creatine, urea, and NH3 present are removed by the blanking and NADPH systems. On adding reagent 2, CyDTA inactivates ICD to inhibit the NADPH system. Simultaneously, the creatinine (1 mol) in the specimen is hydrolyzed into creatine by CRNase, and then releases NADP+ (2 mol) through the blanking system. Our optimized method can determine creatinine linearly up to 500 mg/L, with within-day CVs < 1.2% and day-to-day CVs < 2.7%.

Reference Values of Serum and Urine Creatinine, and of Creatinine Clearance by a New Enzymatic Method

1992 ◽  
Vol 29 (5) ◽  
pp. 523-528 ◽  
Author(s):  
O Sugita ◽  
K Uchiyama ◽  
T Yamada ◽  
T Sato ◽  
M Okada ◽  
...  
Keyword(s):  
Creatinine Clearance ◽  
Reference Intervals ◽  
Japanese Children ◽  
Mean Values ◽  
Urine Creatinine ◽  
Coefficients Of Variation ◽  
Analytical Recovery ◽  
Enzymatic Procedure ◽  
Serum And Urine

A new, totally enzymatic procedure for the determination of creatinine in serum and urine, using creatinine amidohydrolase, creatine amidinohydrolase, sarcosine oxidase and formaldehyde dehydrogenase is described. The assay was adapted to a discontinuous analyser with each analysis requiring only 20 μL of serum or 3 μL of urine. Analytical recovery of creatinine in serum and urine averaged 100·6%. Within-run and between-run precision studies gave coefficients of variation of 1·1% and 1·8%, respectively, for a serum with mean values of 83 μmol/L (9·4 mg/L) creatinine. Creatinine concentrations in serum and urine were measured by this procedure, in Japanese children and adults. The reference intervals for serum creatinine concentrations in adults were 55–96 μmol/L (6·2–10·9 mg/L) in men and 40–66 μmol/L (4·5–7·5 mg/L) in women, and for urine, 9·46–19·01 mmol/day (1070–2150 mg/day) in men and 6·75–10·61 mmol/day (764–1200 mg/day) in women. The reference intervals of creatinine clearance were 88·0–176·4 mL/min in men and 75·7–173·0 mL/min in women.

Enzymic assay of creatinine in serum and urine with creatinine iminohydrolase and glutamate dehydrogenase.

1982 ◽  
Vol 28 (7) ◽  
pp. 1461-1464 ◽  
Author(s):  
E Tanganelli ◽  
L Prencipe ◽  
D Bassi ◽  
S Cambiaghi ◽  
E Murador
Keyword(s):  
Glutamate Dehydrogenase ◽  
Reagent Blank ◽  
Standard Curve ◽  
Analytical Recovery ◽  
Kinetic Test ◽  
Fuller’S Earth ◽  
Enzymic Assay ◽  
Serum And Urine

Abstract We describe an assay for creatinine in which it is converted by creatinine iminohydrolase (EC 3.5.4.21) into ammonia and N-methylhydantoin. The ammonia is subsequently assayed by use of alpha-ketoglutarate and glutamate dehydrogenase (EC 1.4.1.3). Use of NADPH as coenzyme eliminates all interferences from endogenous reactions. Endogenous ammonia in the sample is eliminated during a preincubation. The reaction reaches the endpoint in 15 min at working temperatures of 20-37 degrees C. No sample blank or reagent blank is needed. The standard curve is linear at least to 884 mumol (100 mg) of creatinine per liter. Average analytical recovery of creatinine in serum and urine is 99%. Within-run and between-run CVs are less than or equal to 2% and less than or equal to 6% for creatinine values of 335 mumol/L (38 mg/L) and 80 mumol/L (0 mg/L), respectively. Results by the described method (y) compare well with those by Jaffé's kinetic test (y = 1.01x -- 12.8), Berthelot/AutoAnalyzer method after treatment with immobilized creatinine iminohydrolase (y = 0.987x -- 13.2), Jaffé's test run on the SMA 12/60 (y = 1.011x -- 5.8), the Wahlefeld method (y = 1.014x -- 0.88), and Jaffé's test after deproteinization and absorption on fuller's earth (y = 0.985x -- 3.08). The method may be suitable for discrete, including centrifugal, automation.

Fully Enzymatic Colorimetric Assay of Serum and Urine Creatinine Which Obviates the Need for Sample Blank Measurements

1988 ◽  
Vol 21 (6) ◽  
pp. 1009-1017 ◽  
Author(s):  
J. Siedel ◽  
R. Deeg ◽  
H. Seidel ◽  
H. Möllering ◽  
J. Staepels ◽  
...  
Keyword(s):  
Colorimetric Assay ◽  
Urine Creatinine ◽  
Serum And Urine
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