Organ culture of suckling rat intestine: Comparative study of various hormones on brush border enzymes

In Vitro ◽  
1982 ◽  
Vol 18 (4) ◽  
pp. 339-346 ◽  
Author(s):  
P. M. Simon ◽  
M. Kedinger ◽  
F. Raul ◽  
J. F. Grenier ◽  
K. Haffen
Keyword(s):  
Organ Culture ◽  
Comparative Study ◽  
Brush Border ◽  
Rat Intestine ◽  
Brush Border Enzymes

Control of Brush Border Enzymes by Dexamethasone in the Fetal Rat Intestine Cultured In Vitro

1982 ◽  
Vol 1 (2) ◽  
pp. 257-266 ◽  
Author(s):  
P. M. Simon-Assmann ◽  
M. Kedinger ◽  
J. F. Grenier ◽  
K. Haffen
Keyword(s):  
Brush Border ◽  
Rat Intestine ◽  
Brush Border Enzymes ◽  
Fetal Rat

Expression of brush border enzymes in response to lead exposure in rat intestine

2005 ◽  
Vol 25 (5) ◽  
pp. 361-364 ◽  
Author(s):  
Priya Kapur ◽  
Kamaljit Kaur ◽  
Akhtar Mahmood
Keyword(s):  
Lead Exposure ◽  
Brush Border ◽  
Rat Intestine ◽  
Brush Border Enzymes

Effect of hydrocortisone on the maturation of human foetal kidney explants in serum-free organ culture

1989 ◽  
Vol 67 (2-3) ◽  
pp. 121-127 ◽  
Author(s):  
Lyne Bertrand ◽  
Normand Brière
Keyword(s):  
Organ Culture ◽  
Brush Border ◽  
In Vitro Maturation ◽  
Kidney Development ◽  
Thymidine Incorporation ◽  
Brush Border Enzymes ◽  
Serum Free ◽  
Foetal Kidney ◽  
Developing Kidney

The effect of hydrocortisone on the in vitro maturation of human foetal kidney was investigated. Following legal therapeutic abortions, explants of renal cortex from foetuses aged 13–18 weeks were cultured for 5 days in serum-free Leibovitz's L-15 medium at 37 °C in a mixture of 95% air – 5% CO2, without hormone (controls) or with hydrocortisone at concentrations of 12.5, 25, or 50 ng/mL, which are the levels representative of different gestational periods. During the studied period of culture, the overall architecture of the renal structures was preserved without any evident signs of nephrogenesis induced by hydrocortisone. DNA synthesis was measured by incorporation of [3H]thymidine and was stimulated on day 5 by 80% with the addition of hydrocortisone at 12.5 ng/mL, and by 131% with 50 ng/mL. In autoradiograms, the sites of [3H]thymidine incorporation were the same after hydrocortisone addition, but the number of labelled nuclei was higher in 5-day explants supplemented with hydrocortisone at 50 ng/mL. The activities of some brush border enzymes (leucylnaphthylamidase, maltase, and alkaline phosphatase) were not influenced by hydrocortisone when compared with controls. Trehalase activity was decreased on day 5 with 12.5 and 50 ng/mL. A concentration of 12.5 ng/mL diminished γ-glutamyltransferase activity by 29% on day 5. The incorporation of [3H]leucine into proteins was not influenced by any concentration of the glucocorticoid hormone. This study indicates that hydrocortisone directly influences cell proliferation and certain brush border enzymic activities in human developing kidney maintained in organ culture.Key words: hydrocortisone, organ culture, human foetus, kidney, development.

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