The isolation and purification of diamine oxidase of pea seedlings and pig liver

1996 ◽  
Vol 56 (3) ◽  
pp. 235-241 ◽  
Author(s):  
Yüksel A. Güvenilir ◽  
Nüran Deveci
Keyword(s):  
Diamine Oxidase ◽  
Pig Liver ◽  
Isolation And Purification ◽  
Pea Seedlings

Improved Chromatographic Purification of Pea Seedlings Diamine Oxidase

1993 ◽  
Vol 23 (3) ◽  
pp. 303-319 ◽  
Author(s):  
M. Wimmerovd ◽  
Z. Glatz ◽  
O. Janiczek ◽  
L. Macholdn
Keyword(s):  
Diamine Oxidase ◽  
Chromatographic Purification ◽  
Pea Seedlings

Isolation and Purification of Pig Liver Catalase

1971 ◽  
Vol 74 (2) ◽  
pp. 172 ◽  
Author(s):  
R. C. Srivastava ◽  
Claudia K. Brundage ◽  
Max A. Pecht ◽  
W. Robert Carper
Keyword(s):  
Pig Liver ◽  
Isolation And Purification

scholarly journals Modulation of polyamine catabolism in pea seedlings by calcium during salinity stress

2012 ◽  
Vol 48 (No. 2) ◽  
pp. 53-64 ◽  
Author(s):  
J. Piterková ◽  
L. Luhová ◽  
L. Zajoncová ◽  
M. Šebela ◽  
M. Petřivalský
Keyword(s):  
Biomass Production ◽  
Salinity Stress ◽  
Diamine Oxidase ◽  
Polyamine Catabolism ◽  
Aminoaldehyde Dehydrogenase ◽  
Pea Seedlings ◽  
Polyamine Level ◽  
Activity Of Enzymes ◽  
Positive Effect

The relation of polyamine catabolism in the response of Pisum sativum to salinity stress was investigated. Pea seedlings were grown in increasing concentrations of Na<sup>+</sup> or K<sup>+</sup> or at different concentration ratios of these ions. We studied the effect of Ca<sup>2+</sup> supplementation on plants exposed to salinity stress. The parameters measured in the roots and shoots of pea seedlings included biomass production, levels of Na<sup>+</sup>, K<sup>+</sup>, Ca<sup>2+</sup> and polyamines and activity of enzymes of polyamine catabolism: diamine oxidase, aminoaldehyde dehydrogenase and peroxidases. Salinity induced increased polyamine levels and higher activity of enzymes participating in polyamine degradation. Supplementation of Ca<sup>2+</sup> had a positive effect on biomass production and in most cases it stabilised both the polyamine level and the activity of the studied enzymes. Our results confirm the role of aminoaldehyde dehydrogenase and polyamine catabolism in defence mechanisms of pea plants under salinity stress.

Photoregulation of Diamine Oxidase from Pea Seedlings

1995 ◽  
Vol 146 (1-2) ◽  
pp. 108-114 ◽  
Author(s):  
P. Joseph ◽  
S.K. Srivastava
Keyword(s):  
Diamine Oxidase ◽  
Pea Seedlings

scholarly journals Involvement of Diamine Oxidase and Peroxidase in Insolubilization of the Extracellular Matrix: Implications for Pea Nodule Initiation by Rhizobium leguminosarum

2000 ◽  
Vol 13 (4) ◽  
pp. 413-420 ◽  
Author(s):  
J.-P. Wisniewski ◽  
E. A. Rathbun ◽  
J. P. Knox ◽  
N. J. Brewin
Keyword(s):  
Diamine Oxidase ◽  
Rhizobium Leguminosarum ◽  
Plant Cell Wall ◽  
Extracellular Enzymes ◽  
Host Cells ◽  
Soluble Form ◽  
Post Inoculation ◽  
Root Tips ◽  
Pea Seedlings ◽  
Infection Threads

Rhizobium leguminosarum colonizes host cells and tissues through infection threads, which are tubular in-growths of the plant cell wall. Monoclonal antibody MAC265 recognizes a plant matrix glycoprotein (MGP) associated with the lumen of these infection threads. This glycoprotein is also released in soluble form from the root tips of pea seedlings. In the presence of hydrogen peroxide, release of glycoprotein from root tips was not observed. Extractability from root tips was therefore used as the basis for investigating the peroxide-driven insolubilization of MGP and the possible involvement of two extracellular enzymes, peroxidase (POD) and diamine oxidase (DAO), was investigated. Release of MGP from root tips was enhanced by application of POD and DAO inhibitors (salicylhy-droxamic acid and o-phenanthroline, respectively). Furthermore, release of MGP was inhibited by pretreatment of roots with putrescine (the substrate of DAO) and also by application of a partially purified extract of DAO from pea shoots. Following inoculation of pea roots with R. le-guminosarum, elevated levels of DAO transcript were observed by reverse transcriptase-polymerase chain reaction (RT-PCR), but these then dropped to a low level from 4 to 10 days post inoculation, rising again in more mature nodules. In situ hybridization studies indicated that the bulk of the transcription was associated with the infected tissue in the center of the nodule. On the basis of these observations, we postulate that DAO may be involved in the peroxide-driven hardening of MGP in the lumen of infection threads and in the intercellular matrix.

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