Copper-specific damage in human erythrocytes exposed to oxidative stress

1997 ◽  
Vol 56 (2) ◽  
pp. 231-240 ◽  
Author(s):  
David A. Clopton ◽  
Paul Saltman
Keyword(s):  
Oxidative Stress ◽  
Human Erythrocytes

Acrylamide-induced oxidative stress in human erythrocytes

2009 ◽  
Vol 28 (10) ◽  
pp. 611-617 ◽  
Author(s):  
Betul Catalgol ◽  
Gül Özhan ◽  
Buket Alpertunga
Keyword(s):  
Oxidative Stress ◽  
Reduced Glutathione ◽  
Human Erythrocytes ◽  
Antioxidant Enzyme Activities ◽  
Total Glutathione ◽  
Sod Activity ◽  
Spectrophotometric Methods ◽  
Low Concentrations ◽  
Different Doses

Acrylamide (AA), a widely used industrial chemical, is shown to be neurotoxic, mutagenic and carcinogenic. This study was carried out to investigate the effects of different doses of AA on lipid peroxidation (LPO), haemolysis, methaemoglobin (MetHb) and antioxidant system in human erythrocytes in vitro. Erythrocyte solutions were incubated with 0.10, 0.25, 0.50 and 1.00 mM of AA at 37°C for 1 hour. At the end of the incubation, malondialdehyde (MDA), an end product of LPO, was determined by liquid chromatography (LC) while total glutathione, reduced glutathione (GSH) levels, activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) enzymes and the rates of haemolysis and MetHb were determined by spectrophotometric methods. All of the studied concentrations of AA increased MetHb formation and SOD activity, and induced MDA formation and haemolysis due to the destruction of erythrocyte cell membrane. AA caused a decrease in the activities of GSH-Px, CAT and GSH levels. However, these effects of AA were seen only at higher concentrations than AA intake estimated for populations in many countries. We suggest that LPO process may not be involved in the toxic effects of AA in low concentrations, although the present results showed that the studied concentrations of AA exert deteriorating effects on antioxidant enzyme activities, LPO process and haemolysis.

scholarly journals Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
David Meléndez-Martínez ◽  
Juan Manuel Muñoz ◽  
Guillermo Barraza-Garza ◽  
Martha Sandra Cruz-Peréz ◽  
Ana Gatica-Colima ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Erythrocytes

Protective effect of lutein against benzo(a)pyrene-induced oxidative stress in human erythrocytes

2012 ◽  
Vol 30 (3) ◽  
pp. 284-293 ◽  
Author(s):  
Viswanadha VijayaPadma ◽  
Periasamy Ramyaa ◽  
Dhayalan Pavithra ◽  
Rajashree Krishnasamy
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Human Erythrocytes

Malathion-induced oxidative stress in human erythrocytes and the protective effect of vitamins C and Ein vitro

2009 ◽  
Vol 24 (3) ◽  
pp. 235-242 ◽  
Author(s):  
Dilek Durak ◽  
Fatma Gökce Uzun ◽  
Suna Kalender ◽  
Ayse Ogutcu ◽  
Meltem Uzunhisarcikli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Human Erythrocytes

scholarly journals Pyridoxine Decreases Oxidative Stress on Human Erythrocyte Membrane Protein in vitro

2019 ◽  
Vol 13 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Margarita Velásquez ◽  
Darío Méndez ◽  
Carlos Moneriz
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Membrane Protein ◽  
Oxidative Damage ◽  
Erythrocyte Membrane ◽  
Cumene Hydroperoxide ◽  
Protein Carbonylation ◽  
Human Erythrocytes ◽  
Red Cell Membrane

Background: Pyridoxine has reduction and prevention against the levels of reactive oxygen species in in vitro studies. However, the biochemical mechanism that explains this behavior has not yet been fully clarified. Objective: To evaluate the effect of pyridoxine against oxidative damage on the membrane of human erythrocytes. Methods: Cumene hydroperoxide was used to induce oxidative stress in protein and lipid. Human erythrocytes were incubated with pyridoxine and cumene hydroperoxide, either alone or together for 8 h. Oxidative damage was determined by measuring lipid peroxidation and membrane protein carbonylation. Results: The results indicate that the malondialdehyde concentration decreased with increasing concentration of pyridoxine. The membrane protein content also decreased with increasing concentration of vitamin B6, which was confirmed by the decreased signal intensity in the western blot when compared to control without pyridoxine. Results demonstrate that pyridoxine can significantly decrease lipid peroxidation and protein carbonylation in red cell membrane exposed to high concentrations of oxidant agent. Conclusion: Pyridoxine showed a protective effect against the oxidative stress in human erythrocytes in vitro, inhibiting the carbonylation and the oxidative damage of erythrocyte membrane proteins. To date, such an effect has not yet been reported in terms of protein oxidation.

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