The competition between transferrins labeled with59Fe,65Zn, and54Mn for the binding sites on lactating mouse mammary gland cells

1998 ◽  
Vol 61 (2) ◽  
pp. 181-191 ◽  
Author(s):  
Dimiter Moutafchiev ◽  
Ljuben Sirakov ◽  
Panayot Bontchev
Keyword(s):  
Mammary Gland ◽  
Binding Sites ◽  
Mouse Mammary Gland ◽  
Gland Cells ◽  
Mammary Gland Cells

scholarly journals Apoptosis and metastasis inhibitory potential of pineapple vinegar against mouse mammary gland cells in vitro and in vivo

2019 ◽  
Vol 16 (1) ◽  
Author(s):  
Nurul Elyani Mohamad ◽  
Nadiah Abu ◽  
Swee Keong Yeap ◽  
Kian Lam Lim ◽  
Muhammad Firdaus Romli ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mouse Mammary Gland ◽  
Gland Cells ◽  
Inhibitory Potential ◽  
Mammary Gland Cells

scholarly journals Comprehensive multi-omics analysis uncovers a group of TGF-β-regulated genes among lncRNA EPR direct transcriptional targets

2020 ◽  
Vol 48 (16) ◽  
pp. 9053-9066 ◽  
Author(s):  
Ettore Zapparoli ◽  
Paola Briata ◽  
Martina Rossi ◽  
Lorenzo Brondolo ◽  
Gabriele Bucci ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Binding Sites ◽  
Epithelial To Mesenchymal Transition ◽  
Tumor Formation ◽  
Mesenchymal Transition ◽  
Transcriptional Targets ◽  
Gland Cells ◽  
Chromatin Interactions ◽  
Mammary Gland Cells

Abstract Long non-coding RNAs (lncRNAs) can affect multiple layers of gene expression to control crucial cellular functions. We have previously demonstrated that the lncRNA EPR, by controlling gene expression at different levels, affects cell proliferation and migration in cultured mammary gland cells and impairs breast tumor formation in an orthotopic transplant model in mice. Here, we used ChIRP-Seq to identify EPR binding sites on chromatin of NMuMG mammary gland cells overexpressing EPR and identified its trans binding sites in the genome. Then, with the purpose of relating EPR/chromatin interactions to the reshaping of the epitranscriptome landscape, we profiled histone activation marks at promoter/enhancer regions by ChIP-Seq. Finally, we integrated data derived from ChIRP-Seq, ChIP-Seq as well as RNA-Seq in a comprehensive analysis and we selected a group of bona fide direct transcriptional targets of EPR. Among them, we identified a subset of EPR targets whose expression is controlled by TGF-β with one of them—Arrdc3—being able to modulate Epithelial to Mesenchymal Transition. This experimental framework allowed us to correlate lncRNA/chromatin interactions with the real outcome of gene expression and to start defining the gene network regulated by EPR as a component of the TGF-β pathway.

scholarly journals CD24 staining of mouse mammary gland cells defines luminal epithelial, myoepithelial/basal and non-epithelial cells

2005 ◽  
Vol 8 (1) ◽  
Author(s):  
Katherine E Sleeman ◽  
Howard Kendrick ◽  
Alan Ashworth ◽  
Clare M Isacke ◽  
Matthew J Smalley
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Mouse Mammary Gland ◽  
Gland Cells ◽  
Mammary Gland Cells
Sign in / Sign up

Export Citation Format

Share Document