Enzymatic hydrolysis of starch in water-immiscible organic solvent, two-phase systems

1995 ◽  
Vol 55 (2) ◽  
pp. 75-86 ◽  
Author(s):  
Takashi Morita ◽  
Isao Karube
Keyword(s):  
Enzymatic Hydrolysis ◽  
Organic Solvent ◽  
Two Phase ◽  
Phase Systems ◽  
Hydrolysis Of

scholarly journals Enzymatic Hydrolysis of Sugarcane Bagasse in Aqueous Two-Phase Systems (ATPS): Exploration and Conceptual Process Design

2020 ◽  
Vol 8 ◽  
Author(s):  
Bianca Consorti Bussamra ◽  
Paulus Meerman ◽  
Vidhvath Viswanathan ◽  
Solange I. Mussatto ◽  
Aline Carvalho da Costa ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Process Design ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Conceptual Process Design ◽  
Hydrolysis Of

Extractive bioconversions in aqueous two-phase systems: Enzymatic hydrolysis of casein proteins

1992 ◽  
Vol 40 (2) ◽  
pp. 195-206 ◽  
Author(s):  
S. Mukataka ◽  
C. A. Haynes ◽  
J. M. Prausnitz ◽  
H. W. Blanch
Keyword(s):  
Enzymatic Hydrolysis ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Hydrolysis Of

Enzymatic hydrolysis of bovine hide and recovery of collagen hydrolysate in aqueous two-phase systems

2012 ◽  
Vol 89 ◽  
pp. 282-287 ◽  
Author(s):  
Pitchaivelu Selvakumar ◽  
Tau Chuan Ling ◽  
Anthony D. Covington ◽  
Andrew Lyddiatt
Keyword(s):  
Enzymatic Hydrolysis ◽  
Two Phase ◽  
Collagen Hydrolysate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Bovine Hide ◽  
Hydrolysis Of

Study of reactions in two-phase systems hydrolysis of triphenylmethyl chloride in water-organic solvent systems

1982 ◽  
Vol 47 (11) ◽  
pp. 2904-2911 ◽  
Author(s):  
Jaroslav Šilhánek ◽  
Lenka Konrádová ◽  
Olga Šimečková ◽  
Josef Horák
Keyword(s):  
Organic Solvent ◽  
Aqueous Phase ◽  
Reaction Rate ◽  
Two Phase ◽  
Rate Determining Step ◽  
Hydrolytic Reaction ◽  
Rate Of Hydrolysis ◽  
Solvent Systems ◽  
Phase Systems ◽  
Hydrolysis Of

The rate of hydrolysis was studied for triphenylmethyl chloride in water-organic solvent systems with an unstirred interface of a known area. The hydrolytic reaction was found to take place at the interface only. The temperature dependence of the reaction rate indicates that at lower temperatures (up to 60 °C) the rate-determining step is the chemical reaction, whereas at higher temperatures a deviation from the Arrhenius type dependence is observed, which along with the low activation energy value points to the diffusion as the governing phenomenon. The effect of salts added to the aqueous phase is consistent with the expected behaviour for the SN1 mechanism, but suprising is the inhibiting effect od some quaternary ammonium salts. In the study of the effect of the organic solvent, the reaction rate was found to correlate satisfactorily with the solubility of the organic solvent in water, but no correlation was established with the solubility of water in the solvent. From the results obtained it can be inferred that the reaction site is the laminary layer adjacent to the interface from the aqueous phase side.

Enzymatic hydrolysis of β-lactam antibiotics at low pH in a two-phase "aqueous solution - water-immiscible organic solvent" system

2002 ◽  
Vol 80 (6) ◽  
pp. 699-707 ◽  
Author(s):  
Ghermes G Chilov ◽  
Vytas K Švedas
Keyword(s):  
Aqueous Solution ◽  
Enzymatic Hydrolysis ◽  
Organic Solvent ◽  
Solvent System ◽  
Low Ph ◽  
Thermodynamic Evaluation ◽  
Two Phase ◽  
Suggested Approach ◽  
Hydrolysis Of ◽  
Organic Solvent System

The application of the two-phase "aqueous solution – water-immiscible organic solvent" system is suggested not for effective biocatalytic synthesis, but for hydrolytic purposes. Enzymatic hydrolysis of benzylpenicillin and N-phenylacetamidodesacetoxycephalosporanic acid to corresponding antibiotic nuclei 6-aminopenicillanic and 7-aminodesacetoxycephalosporanic acids in a two-phase water–butylacetate system at pH 3–4 is proposed as an alternative to the biocatalytic hydrolysis in an alkaline medium. An experimental study has been performed and a model has been developed, which describes the influence of pH, phase volume ratio, thermodynamic constants, and initial antibiotic concentration on the effectiveness of their hydrolysis in a two-phase "aqueous solution – water-immiscible organic solvent" system. The thermodynamic evaluation of penicillin G and 7-phenylacetamidodesacetoxycephalosporanic acid hydrolysis at low pH in a two-phase aqueous solution – water-immiscible organic solvent system has demonstrated high practical potential. The suggested approach allows for the exclusion of several technological steps during the transformation of natural β-lactam antibiotics to their semi-synthetic analogues: alkaline extraction of the biosynthetic antibiotic from butylacetate followed by its enzymatic hydrolysis at pH 7.5–8.0 and further acidification of the reaction mixture, which results in the precipitation of the antibiotic nucleus. Experimental observations also revealed a specific feature of this process: the kinetic supersaturation of the antibiotic nucleus slows down the attainment of the equilibrium, which should be taken into account when further developing this approach.Key words: enzymatic hydrolysis, β-lactam antibiotic nuclei, two-phase systems, supersaturation, penicillin acylase.

Investigation of Alkaline Hydrogen Peroxide in Aqueous Organic Solvent to Enhance Enzymatic Hydrolysis of Rice Straw

2020 ◽  
Author(s):  
Jaruwan Damaurai ◽  
Thanchanok Preechakun ◽  
Marisa Raita ◽  
Verawat Champreda ◽  
Navadol Laosiripojana
Keyword(s):  
Hydrogen Peroxide ◽  
Enzymatic Hydrolysis ◽  
Organic Solvent ◽  
Rice Straw ◽  
Alkaline Hydrogen Peroxide ◽  
Hydrolysis Of

Enzymatic hydrolysis of soluble starch in a polyethylene glycol-dextran aqueous two-phase system

1990 ◽  
Vol 69 (4) ◽  
pp. 240-243 ◽  
Author(s):  
Kazuhiko Hayashida ◽  
Koji Kunimoto ◽  
Fumihide Shiraishi ◽  
Koei Kawakami ◽  
Yasuhiko Arai
Keyword(s):  
Polyethylene Glycol ◽  
Enzymatic Hydrolysis ◽  
Soluble Starch ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Hydrolysis Of
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