Decreased interferon binding capacity of peripheral blood mononuclear cells during interferon treatment of chronic hepatitis B with special reference to (2′-5′) oligoadenylate synthetase activity

1989 ◽  
Vol 24 (6) ◽  
pp. 670-675 ◽  
Author(s):  
Michiko Shindo ◽  
Akira Iwata ◽  
Tadao Okuno ◽  
Masayuki Matsumoto ◽  
Makoto Takeda ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Peripheral Blood Mononuclear Cells ◽  
Mononuclear Cells ◽  
Binding Capacity ◽  
Synthetase Activity ◽  
Interferon Treatment ◽  
Oligoadenylate Synthetase ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Zhongsi Hong ◽  
Haiyu Hong ◽  
Jian Liu ◽  
Xiaobin Zheng ◽  
Mingxing Huang ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Luciferase Activity ◽  
Peripheral Blood Mononuclear ◽  
Protein Levels ◽  
Qrt Pcr ◽  
Blood Mononuclear Cells

Aims. This study aimed to investigate miR-106a expression in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients and to analyze the function of miR-106a.Materials and Methods. miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The luciferase activity assays were used to determine whether miR-106a binds to 3′UTR of IL-8. miR-106a mimics and inhibitors were transfected into healthy PBMCs. IL-8 mRNA and protein levels were detected and determined by qRT-PCR and ELISA, respectively.Results. The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients. IL-8 was augmented in CHB patients and was inversely correlated with miR-106a levels. The luciferase activity assays indicated that IL-8 is a target of miR-106a. Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.Conclusions. This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.

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