Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining

2001 ◽  
Vol 19 (4) ◽  
pp. 299-306 ◽  
Author(s):  
S. Creste ◽  
A. Tulmann Neto ◽  
A. Figueira
Keyword(s):  
Silver Staining ◽  
Sequence Repeat ◽  
Single Sequence Repeat ◽  
Single Sequence

Inter-single-sequence-repeat-PCR typing as a new tool for identification of Microsporum canis strains

2005 ◽  
Vol 39 (1) ◽  
pp. 17-21 ◽  
Author(s):  
José Cano ◽  
Antonio Rezusta ◽  
María Solé ◽  
Joaquina Gil ◽  
María Carmen Rubio ◽  
...  
Keyword(s):  
Microsporum Canis ◽  
Sequence Repeat ◽  
Single Sequence Repeat ◽  
Single Sequence

scholarly journals Propagation management methods have altered the genetic variability of two traditional mango varieties in Myanmar, as revealed by SSR

2011 ◽  
Vol 9 (3) ◽  
pp. 404-410 ◽  
Author(s):  
R. Hirano ◽  
H. Ishii ◽  
Than Htun Oo ◽  
S. A. Gilani ◽  
A. Kikuchi ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Genetic Background ◽  
Clonal Propagation ◽  
Mangifera Indica ◽  
Sequence Repeat ◽  
Single Sequence Repeat ◽  
Principal Coordinates ◽  
The Relationship ◽  
Single Sequence ◽  
Cultivation History

Mango (Mangifera indica L.) is an important fruit crop with a long cultivation history in Myanmar. This study evaluated the genetic variation within two economically important traditional varieties, ‘Yin Kwe’ and ‘Sein Ta Lone’, and the relationship between genetic variation and propagation practices. Genetic variation was estimated by genotyping 94 individuals with 12 single sequence repeat markers. ‘Yin Kwe’ (n = 53) showed higher levels of observed heterozygosity (Ho = 0.59) and average genetic distance among individuals (Da = 0.29) than did ‘Sein Ta Lone’ (n = 41; Ho = 0.45; Da = 0.09). The differences between the two varieties at the DNA level were significant (Fst = 0.44). The broader genetic background in ‘Yin Kwe’ compared with ‘Sein Ta Lone’ was also demonstrated by neighbour-joining and principal coordinates analyses. Differences in variety uses and propagation practices were determined by interviewing local specialists in Lower Myanmar (southern Myanmar). ‘Yin Kwe’ was often used as a rootstock for ‘Sein Ta Lone’. Clonal propagation by grafting was observed frequently for ‘Sein Ta Lone’ but never for ‘Yin Kwe’. The differences in genetic variation between these two varieties might have been caused by the propagation practices for each variety, which result from their respective uses.

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