A novel chalcone synthase gene fromPhalaenopsis orchid that alters floral morphology in transgenic tobacco plants

2005 ◽  
Vol 23 (2) ◽  
pp. 193-194 ◽  
Author(s):  
Ying-Ying Han ◽  
Feng Ming ◽  
Jing-Wen Wang ◽  
Ming-Ming Ye ◽  
Da-Leng Shen
Keyword(s):  
Transgenic Tobacco ◽  
Chalcone Synthase ◽  
Floral Morphology ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Chalcone Synthase Gene

scholarly journals Overexpression of Chalcone Synthase Improves Flavonoid Accumulation and Drought Tolerance in Tobacco

2019 ◽  
Author(s):  
Ben Hu ◽  
Heng Yao ◽  
Xiaojun Peng ◽  
Ran Wang ◽  
Feng Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Chalcone Synthase ◽  
Redox Balance ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Cellular Redox ◽  
Key Enzyme

Flavonoids are major secondary metabolites in plants, which play important roles in maintaining the cellular redox balance in cells. Chalcone synthase (CHS) is the key enzyme in the flavonoids biosynthesis pathway, and has been proved to monitor the changes to drought stress tolerance. In this work, we overexpressed a CHS gene in tobacco (Nicotiana tabacum). The transgenic tobacco plants were more tolerant than the control plants to drought stress. The transcription levels of the key genes involved in the flavonoids pathway and the contents of seven flavonoids were also significantly raised in the transgenic tobacco plants. In addition, overexpression of the CHS gene lead to a lower concentration of the oxidative stress product malondialdehyde. Overall, the NtCHS gene studied in this work was considered as a candidate gene for genetic engineering to enhance drought tolerance of plants and improve response to oxidative stress.

scholarly journals Overexpression of chalcone synthase gene improves flavonoid accumulation and drought tolerance in tobacco

2020 ◽  
Author(s):  
Ben Hu ◽  
Heng Yao ◽  
Yulong Gao ◽  
Ran Wang ◽  
Feng Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Chalcone Synthase ◽  
Phylogenetic Analyses ◽  
Redox Balance ◽  
Transgenic Tobacco Plants ◽  
Flavonoid Pathway ◽  
Tobacco Plants

Abstract Flavonoids are important secondary metabolites in plants that play important roles in maintaining the cellular redox balance of cells. Chalcone synthase (CHS) is the key enzyme in the flavonoid biosynthesis pathway and has been found to monitor changes due to drought stress tolerance. In this study, a CHS gene in tobacco ( Nicotiana tabacum ) was overexpressed. Results revealed that transgenic tobacco plants were more tolerant than control plants to drought stress. Transcription levels of the key genes involved in the flavonoid pathway and the contents of seven flavonoids significantly increased in transgenic tobacco plants ( p < 0.01). Overexpression of the CHS gene led to lower concentrations of the oxidative stress product, malondialdehyde (MDA). Additionally, 11 CHS family genes were mined from the tobacco genome. Based on the phylogenetic tree, these genes split into two groups with eight genes clustered together with the bona fide Arabidopsis CHS gene, suggesting that those tobacco genes are CHS genes. Further phylogenetic analyses indicated that the tobacco CHS genes grouped further into three independent clades with the cloned tobacco CHS gene located within Clade iii. The tobacco CHS family genes exhibited a highly conserved CDS length, pI, and molecular weight of the encoded peptides. All CHS peptides contained two conserved domains, and the genes harbored two or three exons. Based on the results of this study, the NtCHS gene is considered a possible candidate gene for genetically engineering enhanced drought tolerance and improved responses to oxidative stress in plants.

scholarly journals Overexpression of chalcone synthase gene improves flavonoid accumulation and drought tolerance in tobacco

2019 ◽  
Author(s):  
Ben Hu ◽  
Heng Yao ◽  
Yulong Gao ◽  
Ran Wang ◽  
Feng Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Chalcone Synthase ◽  
Phylogenetic Analyses ◽  
Redox Balance ◽  
Transgenic Tobacco Plants ◽  
Flavonoid Pathway ◽  
Tobacco Plants

Abstract Background: Flavonoids are important secondary metabolites in plants that play important roles in maintaining the cellular redox balance of cells. Chalcone synthase (CHS) is the key enzyme in the flavonoid biosynthesis pathway and has been found to monitor changes due to drought stress tolerance.Results: In this study, a CHS gene in tobacco (Nicotiana tabacum) was overexpressed. Results revealed that transgenic tobacco plants were more tolerant than control plants to drought stress. Transcription levels of the key genes involved in the flavonoid pathway and the contents of seven flavonoids significantly increased in transgenic tobacco plants (p < 0.01). Overexpression of the CHS gene led to lower concentrations of the oxidative stress product, malondialdehyde (MDA). Additionally, 11 CHS family genes were mined from the tobacco genome. Based on the phylogenetic tree, these genes split into two groups with eight genes clustered together with the bona fide Arabidopsis CHS gene, suggesting that those tobacco genes are CHS genes. Further phylogenetic analyses indicated that the tobacco CHS genes grouped further into three independent clades with the cloned tobacco CHS gene located within Clade iii. The tobacco CHS family genes exhibited a highly conserved CDS length, pI, and molecular weight of the encoded peptides. All CHS peptides contained two conserved domains, and the genes harbored two or three exons.Conclusions: Based on the results of this study, the NtCHS gene is considered a possible candidate gene for genetically engineering enhanced drought tolerance and improved responses to oxidative stress in plants.

Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells

2005 ◽  
Vol 14 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Helga Schinkel ◽  
Andreas Schiermeyer ◽  
Raphael Soeur ◽  
Rainer Fischer ◽  
Stefan Schillberg
Keyword(s):  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Suspension Cells ◽  
Tobacco Plants ◽  
Recombinant Thrombomodulin

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

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