Progeny Differences of Hinoki (Chamaecyparis obtusa) and Sawara (C. pisifera) against Resinous Stem Canker Disease and Spatial Distribution of Damage (Disease Severity) in a Progeny Test

1998 ◽  
Vol 3 (4) ◽  
pp. 237-242 ◽  
Author(s):  
Makoto Takahashi ◽  
Minoru Mukouda ◽  
Kenji Nishimura
IAWA Journal ◽  
1998 ◽  
Vol 19 (2) ◽  
pp. 181-189 ◽  
Author(s):  
Keiko Kuroda

Trunks of Chamaecyparis obtusa were injured to examine seasonal differences in traumatic resin canal formation in secondary phloem. Even after wounding during winter, differentiation of axial parenchyma into epithelium was initiated, and vertical resin canals formed. After winter wounding, resin canal development was slower and the tangential extent of resin canals was narrower than after spring wounding, and it took one to two months until resin secretion began. After spring wounding, the sites of resin canal formation were the 1- and 2-year-old annual rings of phloem. In August, the location of resin canal formation shifted into the current and 1-year-old annual ring. Resin canals never formed in secondary phloem areas that were 3 or more years old. In C. obtusa trunks that are affected by the resinous stem canker, numerous tangentiallines of resin canals are found throughout the phloem, not just recent and 1- to 2-year-old phloem. The present research indicates that these many lines of resin canals were not formed at one time, and that the stimuli that induce traumatic resin canals must occur repeatedly over many years. The data on artificial wounding effects are useful for understanding resinous stem canker.


2010 ◽  
Vol 29 (10) ◽  
pp. 1084-1090 ◽  
Author(s):  
Patrick P.J. Haydock ◽  
Thomas Deliopoulos ◽  
Ken Evans ◽  
Stephen T. Minnis

2019 ◽  
Vol 19 (1) ◽  
pp. 82
Author(s):  
Tri Rapani Febbiyanti ◽  
Suryo Wiyono ◽  
Sudirman Yahya ◽  
Widodo Widodo

Analysis of Causative Factors for Canker Disease Epidemic on Rubber Plants in South Sumatra.  Lasiodiplodia theobromae is the cause of stem canker in the rubber plant (Hevea brasiliensis), this is a new disease and has never been reported in Indonesia. Currently, data on environmental factors and cultivation techniques related to the development of stem canker are not available so research needs to be done on this subject. The objective of the study was to analyze environmental factors and cultivation techniques related to stem canker on rubber trees in southern Sumatra. The study was conducted by collecting data on environmental factors and cultivation techniques and then analyzed the association with stem canker. Field observation was carried out at 21 locations in PT. Perkebunan Nusantara VII Business Unit Padang Plawi Bengkulu,  Tulung Buyut Lampung, Musilandas Palembang and Experimental Garden of Sembawa Research Center, Indonesian Rubber Research Institute at Palembang to measure the severity and incidence of this disease. Besides that, the data of cultivation technique and the condition of the garden were taken from the officer at those locations. Soil sample from each location was taken in a composite way, then its physical and chemical components were analyzed. The analysis was conducted to find out the correlation between cultivation and disease severity using chi-square test.  The relationship between chemical and physical factors of the soil with the disease severity was analyzed using a multivariate test of principal component analysis/PCA. Regression analysis was conducted to show the possible relation to the incidence and severity of this disease.  The location, clones, weed control and the number of plants per hectare showed a correlation with the disease severity. There was a positive correlation between incidence and severity of the disease. The highest disease severity occurred at the Sembawa location on clone BPM 24, with the number of plant population per ha was ≥ 555 trees and weed control using herbicide. Content of soil nitrogen, dust, clay and water as well as cation exchange capacity (CEC)were suggested to significantly contribute to the severity of stem canker.


2002 ◽  
Vol 32 (4-5) ◽  
pp. 213-224 ◽  
Author(s):  
T. YAMADA ◽  
M. NAGASHIMA ◽  
C. KAWAGUCHI ◽  
K. OOTSUKI ◽  
N. YANAGITA

Plant Disease ◽  
2012 ◽  
Vol 96 (6) ◽  
pp. 906-906 ◽  
Author(s):  
M. F. Chuang ◽  
H. F. Ni ◽  
H. R. Yang ◽  
S. L. Shu ◽  
S. Y. Lai ◽  
...  

Pitaya (Hylocereus undatus and H. polyrhizus Britt. & Rose), a perennial succulent plant grown in the tropics, is becoming an emerging and important fruit plant in Taiwan. In September of 2009 and 2010, a number of pitaya plants were found to have a distinctive canker on stems. The disease expanded quickly to most commercial planting areas in Taiwan (e.g., Pintung, Chiayi, and Chunghua). Symptoms on the stem were small, circular, sunken, orange spots that developed into cankers. Pycnidia were erumpent from the surface of the cankers and the stems subsequently rotted. After surface disinfestation with 0.1% sodium hypochloride, tissues adjacent to cankers were placed on acidified potato dextrose agar (PDA) and incubated at room temperature for 1 week, after which colonies with dark gray-to-black aerial mycelium grew. Hyphae were branched, septate, and brown and disarticulated into 0- to 1-septate arthrospores. Sporulation was induced by culturing on sterile horsetail tree (Casuarina equisetifolia) leaves. Conidia (12.79 ± 0.72 × 5.14 ± 0.30 μm) from pycnidia were one-celled, hyaline, and ovate. The internal transcribed spacer (ITS) region of ribosomal DNA was PCR amplified with primers ITS1 and ITS4 (2) and sequenced. The sequence (GenBank Accession No. HQ439174) showed 99% identity to Neoscytalidium dimidiatum (Penz.) Crous & Slippers (GenBank Accession No. GQ330903). On the basis of morphology and nucleotide-sequence identity, the isolates were identified as N. dimidiatum (1). Pathogenicity tests were conducted in two replicates by inoculating six surface-sterilized detached stems of pitaya with either mycelium or conidia. Mycelial plugs from 2-day-old cultures (incubated at 25°C under near UV) were inoculated to the detached stems after wounding with a sterile needle. Conidial suspensions (103 conidia/ml in 200 μl) were inoculated to nonwounded stems. Noninoculated controls were treated with sterile medium or water. Stems were then incubated in a plastic box at 100% relative humidity and darkness at 30°C for 2 days. The symptoms described above were observed on inoculated stems at 6 to 14 days postinoculation, whereas control stems did not develop any symptoms. N. dimidiatum was reisolated from symptomatic tissues. To our knowledge, this is the first report of N. dimidiatum causing stem canker of pitaya. References: (1) P. W. Crous et al. Stud. Mycol. 55:235, 2006. (2) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds. Academic Press, New York, 1990.


2018 ◽  
Vol 153 (2) ◽  
pp. 503-515
Author(s):  
Salvatore Vitale ◽  
Laura Luongo ◽  
Etienne G. J. Danchin ◽  
Giovanni Mughini ◽  
Maria Gras ◽  
...  
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