cDNA selection with YACs

1997 ◽  
Vol 8 (3) ◽  
pp. 255-268
Author(s):  
Satish Parimoo
Keyword(s):  
Cdna Selection

cDNA Selection and Other Approaches in Positional Cloning

1995 ◽  
Vol 228 (1) ◽  
pp. 1-17 ◽  
Author(s):  
S. Parimoo ◽  
S.R. Patanjali ◽  
R. Kolluri ◽  
H.X. Xu ◽  
H. Wei ◽  
...  
Keyword(s):  
Positional Cloning ◽  
Cdna Selection

Construction of a cDNA library for a specific region of a chromosome using a novel cDNA selection method utilizing latex particles

Gene ◽  
1995 ◽  
Vol 165 (2) ◽  
pp. 155-161 ◽  
Author(s):  
Nobuaki Hayashida ◽  
Yoshiki Sumi ◽  
Tadashi Wada ◽  
Hiroshi Handa ◽  
Kazuo Shinozaki
Keyword(s):  
Cdna Library ◽  
Selection Method ◽  
Specific Region ◽  
Latex Particles ◽  
Cdna Selection

The use of direct cDNA selection to rapidly and effectively identify genes in the fungus Aspergillus fumigatus

2002 ◽  
Vol 36 (1) ◽  
pp. 59-70 ◽  
Author(s):  
Marco M Kessler ◽  
Debra Aker Willins ◽  
Qiandong Zeng ◽  
Richard G Del Mastro ◽  
Robin Cook ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Cdna Selection

scholarly journals Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair.

1996 ◽  
Vol 16 (9) ◽  
pp. 4832-4841 ◽  
Author(s):  
G M Dolganov ◽  
R S Maser ◽  
A Novikov ◽  
L Tosto ◽  
S Chong ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Myeloid Leukemia ◽  
Protein Complexes ◽  
Selection Strategy ◽  
Mrna Levels ◽  
Multiprotein Complex ◽  
Group Members ◽  
Cdna Selection

In this report, we describe the identification and molecular characterization of a human RAD50 homolog, hRAD50. hRAD50 was included in a collection of cDNAs which were isolated by a direct cDNA selection strategy focused on the chromosomal interval spanning 5q23 to 5q31. Alterations of the 5q23-q31 interval are frequently observed in myelodysplasia and myeloid leukemia. This strategy was thus undertaken to create a detailed genetic map of that region. Saccharomyces cerevisiae RAD50 (ScRAD50) is one of three yeast RAD52 epistasis group members (ScRAD50, ScMRE11, and ScXRS2) in which mutations eliminate meiotic recombination but confer a hyperrecombinational phenotype in mitotic cells. The yeast Rad50, Mre11, and Xrs2 proteins appear to act in a multiprotein complex, consistent with the observation that the corresponding mutants confer essentially identical phenotypes. In this report, we demonstrate that the human Rad50 and Mre11 proteins are stably associated in a protein complex which may include three other proteins. hRAD50 is expressed in all tissues examined, but mRNA levels are significantly higher in the testis. Other human RAD52 epistasis group homologs exhibit this expression pattern, suggesting the involvement of human RAD52 epistasis group proteins in meiotic recombination. Human RAD52 epistasis group proteins are highly conserved and act in protein complexes that are analogous to those of their yeast counterparts. These findings indicate that the function of the RAD52 epistasis group is conserved in human cells.

Isolation of candidate hybrid sterility 1 genes by cDNA selection in a 1.1 megabase pair region on mouse chromosome 17

1997 ◽  
Vol 8 (5) ◽  
pp. 312-316 ◽  
Author(s):  
Zdenek Trachtulec ◽  
Monika Mňuková-Fajdelová ◽  
Renata M. J. Hamvas ◽  
Soña Gregorová ◽  
Werner E. Mayer ◽  
...  
Keyword(s):  
Mouse Chromosome ◽  
Hybrid Sterility ◽  
Chromosome 17 ◽  
Cdna Selection

Use of interspersed repetitive sequences-PCR products for cDNA selection

1995 ◽  
Vol 6 (9) ◽  
pp. 617-622 ◽  
Author(s):  
L. Villard ◽  
E. Passage ◽  
L. Colleaux ◽  
M. Fontes
Keyword(s):  
Repetitive Sequences ◽  
Pcr Products ◽  
Cdna Selection

construction of a transcription map of a 300 kb region around the human G6PD locus by direst cDNA selection

1993 ◽  
Vol 2 (11) ◽  
pp. 1865-1869 ◽  
Author(s):  
Zdenek Sediacek ◽  
Bernhard Korn ◽  
David S. Konecki ◽  
Renate Siebenhaar ◽  
Johannes F. Coy ◽  
...  
Keyword(s):  
Transcription Map ◽  
Cdna Selection
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