Somaclonal variation in tuber traits of potato

2005 ◽  
Vol 48 (3-4) ◽  
pp. 153-165 ◽  
Author(s):  
Ramona Thieme ◽  
Helmut Griess
Keyword(s):  
Somaclonal Variation ◽  
Tuber Traits

scholarly journals Somaclonal variation on in vitro callus culture potato cultivars

2004 ◽  
Vol 22 (2) ◽  
pp. 300-304 ◽  
Author(s):  
Patricia N. Bordallo ◽  
Derly H. Silva ◽  
José Maria ◽  
Cosme D. Cruz ◽  
Elizabeth P. Fontes
Keyword(s):  
Somaclonal Variation ◽  
Culture Media ◽  
Callus Formation ◽  
Potato Cultivars ◽  
Synthetic Seed ◽  
Arbitrary Sequence ◽  
Stem Explants ◽  
Factors Affecting ◽  
High Level

Synthetic seeds can be an alternative for those species in which botanical seeds are not viable. One of the major problems of in vitro plant cultivation is the high level of somaclonal variation. The most common factors affecting somaclonal variation are genotype, explant source, in vitro period and cultivation conditions in which the culture is established. In this work, calli were induced using leaf and stem explants of the commercial potato cultivars Achat, Baraka, Baronesa, Bintje, and Contenda in MS culture media supplemented with 1.65 mM of picloram and 11.5 mM of 2,4-D. Seventy and 90 days after induction, DNA samples of 40 calli were compared concerning the effects of the two explant (leaf and stem) and two growth regulator sources on five potatoes cultivars. A total of 20 arbitrary sequence primers were evaluated. The RAPD pattern generated by these primers suggested a high percentage of polymorphic fragments among the five genotypes, indicating a high level of genetic variation among cultivars. Cultivar Baronesa showed the highest number of polymorphic fragments for all treatments. The cultivar Contenda showed the smallest somaclonal variation, for most of the treatments, except for the treatment which consisted of stem explants, picloram (1.65 mM) application, and a 70-day period of callus formation. 'Contenda' is, therefore, the most suitable cultivar for synthetic seed production.

scholarly journals Assessment of genetic and epigenetic changes during cell culture ageing and relations with somaclonal variation in Coffea arabica

2015 ◽  
Vol 122 (3) ◽  
pp. 517-531 ◽  
Author(s):  
Roberto Bobadilla Landey ◽  
Alberto Cenci ◽  
Romain Guyot ◽  
Benoît Bertrand ◽  
Frédéric Georget ◽  
...  
Keyword(s):  
Cell Culture ◽  
Somaclonal Variation ◽  
Coffea Arabica ◽  
Epigenetic Changes

Development and evaluation of in vitro somaclonal variation in strawberry for improved horticultural traits

2009 ◽  
Vol 122 (3) ◽  
pp. 409-416 ◽  
Author(s):  
M.K. Biswas ◽  
M. Dutt ◽  
U.K. Roy ◽  
R. Islam ◽  
M. Hossain
Keyword(s):  
Somaclonal Variation ◽  
Horticultural Traits

UV-B irradiation effects on pigments and cytological behaviour of callus in sainfoin (Onobrychis viciifolia Scop.)

2018 ◽  
Vol 47 (6) ◽  
pp. 496-501
Author(s):  
Sadegh Mohajer ◽  
Rosna Mat Taha ◽  
Minoo Mohajer ◽  
Nordiyanah Anuar
Keyword(s):  
Somaclonal Variation ◽  
Seed Viability ◽  
Carotenoid Content ◽  
Content Type ◽  
Onobrychis Viciifolia ◽  
Phenolic Contents ◽  
Chlorophyll A And B ◽  
Phytochemical Constituents ◽  
Cellular Behaviour

Purpose This study aims to determine the appropriate irradiation dose for induction of somaclonal variation in mass of unorganized parenchyma cells derived from sainfoin (Onobrychis viciifolia) tissues. Design/methodology/approach To investigate the feasibility of UV-B irradiation (312 nm), seeds and callus of sainfoin were exposed to five different intensities (20-100 per cent) for evaluating the effectiveness of phytochemical constituents and cellular behaviour. Findings Although percentage of seed viability reduced in 20 per cent intensity of UV-B irradiation compared with control seeds, the germination speed significantly enhanced from 3.58 to 5.42. The spectrophotometer analysis confirmed that concentrations of chlorophyll (a and b) decreased after UV-B exposure as compared with control callus. Also, carotenoid content showed significant differences among treated calli. Flavonoid and phenolic contents were observed to gradually increase when the non-irradiated callus subjected to 40 and 60 per cent intensities of UV-B irradiation, respectively. Observation on cellular behaviour such as determination of nuclear and cell areas, mitotic index and chromosomal aberrations were also detected to change in different intensities of UV-B irradiation. Fragmented and aneuploidy aberrations were only observed in exposed cells with more than 40 per cent intensity of UV-B irradiation. Originality/value In reality, radiocytological evaluation was proven to be essential in deducing the effectiveness of UV-B irradiation to induce somaclonal variation in callus tissue of sainfoin.

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