Rat mesencephalic neuronal cells cultured for different periods as a model of dopamine transporter ontogenesis

1995 ◽  
Vol 11 (1-3) ◽  
pp. 111-119 ◽  
Author(s):  
Martin Valchář ◽  
Ingeborg Hanbauer
Keyword(s):  
Dopamine Transporter ◽  
Neuronal Cells ◽  
Cells Cultured

Enhanced neurite outgrowth on electrically conductive carbon aerogel substrates in the presence of an external electric field

Soft Matter ◽  
2021 ◽  
Author(s):  
Martina Rodriguez Sala ◽  
Swetha Chandrasekaran ◽  
Omar Skalli ◽  
Marcus Worsley ◽  
Firouzeh Sabri
Keyword(s):  
Electrical Stimulation ◽  
Electric Field ◽  
Neurite Outgrowth ◽  
External Electric Field ◽  
Neuronal Cells ◽  
Carbon Aerogel ◽  
Cell Behavior ◽  
Electrically Conductive ◽  
Suitable Substrate ◽  
Cells Cultured

It has been established that aerogels are a suitable substrate for neuronal scaffold. In this study, the cell behavior of neuronal cells cultured on aerogels with an electrical stimulation is evaluated.

Levels and sub-cellular distribution of physiologically important metal ions in neuronal cells cultured from chick embryo cerebral cortex

1988 ◽  
Vol 13 (12) ◽  
pp. 1163-1167 ◽  
Author(s):  
G. Tholey ◽  
M. Ledig ◽  
P. Kopp ◽  
L. Sargentini-Maier ◽  
M. Leroy ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Chick Embryo ◽  
Metal Ions ◽  
Neuronal Cells ◽  
Cellular Distribution ◽  
Cells Cultured

DOPAMINE-INDUCED APOPTOSIS IN HUMAN NEURONAL CELLS: INHIBITION BY NUCLEIC ACIDS ANTISENSE TO THE DOPAMINE TRANSPORTER

Neuroscience ◽  
1996 ◽  
Vol 74 (1) ◽  
pp. 39-50 ◽  
Author(s):  
R SIMANTOV ◽  
E BLINDER ◽  
T RATOVITSKI ◽  
M TAUBER ◽  
M GABBAY ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dopamine Transporter ◽  
Neuronal Cells ◽  
Induced Apoptosis ◽  
Human Neuronal Cells

Skin-derived precursors differentiating into dopaminergic neuronal cells in the brains of Parkinson disease model rats

2010 ◽  
Vol 113 (3) ◽  
pp. 648-655 ◽  
Author(s):  
Tetsuhiro Higashida ◽  
Susumu Jitsuki ◽  
Atsuhiko Kubo ◽  
Dai Mitsushima ◽  
Yoshinori Kamiya ◽  
...  
Keyword(s):  
Amino Acids ◽  
High Pressure ◽  
Tyrosine Hydroxylase ◽  
Parkinson Disease ◽  
Dopamine Transporter ◽  
Neuronal Cells ◽  
Disease Model ◽  
Immunohistochemical Analysis ◽  
Von Hippel Lindau ◽  
Differentiated Cells

Object In the authors' previous study, they observed that amino acids 157–171 of von Hippel-Lindau protein (VHL peptide) induced neuronal differentiation of skin-derived precursors. They also noted that transplantation of these differentiated cells into the striata of a Parkinson disease (PD) rat model reduced apomorphine-induced rotations. In the present study, they investigated if these cells produce dopamine in the striatum. Methods Skin-derived precursors were differentiated into neurons using VHL peptide and transplanted into the striata of a PD model of rats. Four weeks after transplantation, a probe was inserted into rat striata and extracellular dopamine was extracted by microdialysis. Dopamine levels were measured by high-pressure liquid chromatography. Brain sections were assessed by immunohistochemical analysis for the presence of tyrosine hydroxylase and dopamine transporter. Results Increased dopamine levels in the striata of the rats were observed after transplantation (p < 0.01), and these were correlated with a reduction in the number of apomorphine-induced rotations (p < 0.05). Skin-derived precursors observed along the tract of transplantation were positive for tyrosine hydroxylase and dopamine transporter. Conclusions This study suggests that transplantation of skin-derived precursors, differentiated into neuronal cells using VHL peptide, can improve PD-like symptoms by enabling production of dopamine in the striata in a PD model of rats.

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