Slow-growth in vitro conservation of potato germplasm at normal propagation temperature

2002 ◽  
Vol 45 (2-4) ◽  
pp. 203-213 ◽  
Author(s):  
J. Gopal ◽  
Anjali Chamail ◽  
Debabrata Sarkar
Keyword(s):  
Slow Growth ◽  
In Vitro Conservation ◽  
Potato Germplasm ◽  
Normal Propagation

Slow growth in vitro conservation of coffee (Coffea spp.)

1992 ◽  
Vol 31 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Anna Bertrand-Desbrunais ◽  
Michel Noirot ◽  
Andr� Charrier
Keyword(s):  
Slow Growth ◽  
In Vitro Conservation

scholarly journals Development of in vitro Slow Growth Culture for Yarn (Dioscoreo alata L.)

2012 ◽  
pp. 79-95 ◽  
Author(s):  
Villaluz Acedo ◽  
Catherine Arradaza
Keyword(s):  
Tissue Culture ◽  
Growth Medium ◽  
Slow Growth ◽  
Genetic Erosion ◽  
Normal Growth ◽  
Culture Technique ◽  
In Vitro Conservation ◽  
Maintenance Cost ◽  
Germplasm Collections

Germplasm collections, the lifeblood of breeding programs, are traditionally maintained in the field. Field genebanks are expensive, subject to genetic erosion, and require several quarantine measures for safe movement of genetic materials. These problems are more serious in long-duration, non-flowering and vegetatively propagated crops like yarn. This study aimed to develop a tissue culture technique for in vitro conservation of yarn germplasm. ’VU-2’ and ‘Kinampay’ varieties were used in establishing the in vitro conservation technique which was then tested to other genotypes. With the tissue culture protocol for yarn propagation developed earlier, the plantlets became overgrown after 2-3 months, requiring frequent subculturing and increasing the cost of maintenance and the risk of microbial contamination. Slow growth culture was tested using MS medium added with 0-10 mg/L abscisic acid (ABA) or 0-7% mannitol or sorbitol. Expectedly, plantlet growth slowed down. However, ABA at higher levels increased mortality of cultures while sorbitol was less effective than mannitol in retarding growth. Mannitol at 4% was found to be the best slow growth medium to maintain the plantlets for 13 months, thereby saving at least 4 times the maintenance cost using the normal growth medium. Tissue viability, morphological stability and tuber yield were not affected. Other genotypes (VU-1, VU-3, VU-4, VU-5, PR5, PR7, PR10 and PR11) responded similarly to the slow growth culture condition.

scholarly journals Slow growth in vitro culture for conservation of Chilotanum potato germplasm

2019 ◽  
Vol 79 (1) ◽  
pp. 26-35 ◽  
Author(s):  
Manuel Muñoz ◽  
Oscar Díaz ◽  
Waleska Reinún ◽  
Annelore Winkler ◽  
Roberto Quevedo
Keyword(s):  
In Vitro Culture ◽  
Slow Growth ◽  
Potato Germplasm

scholarly journals In vitro conservation of Cape gooseberry through slow-growth nodal segment cultures

2018 ◽  
Vol 53 (5) ◽  
pp. 651-655
Author(s):  
Renata Alves Lara Silva Rezende ◽  
Filipe Almendagna Rodrigues ◽  
Ramiro Machado Rezende ◽  
Joyce Dória Rodrigues Soares ◽  
Moacir Pasqual ◽  
...  
Keyword(s):  
Slow Growth ◽  
In Vitro Conservation ◽  
Nodal Segment ◽  
Short Term ◽  
Physalis Peruviana ◽  
Effects Of Temperature ◽  
Osmotic Agents ◽  
Cape Gooseberry

Abstract: The objective of this work was to evaluate the effects of temperature and osmotic agents on the in vitro conservation of Cape gooseberry (Physalis peruviana). Temperatures at 18 and 25°C, as well as the osmotic agents sucrose, mannitol, and sorbitol were tested. A short-term in vitro conservation of Cape gooseberry can be achieved at 18°C, using 30 g L-1 sucrose.

scholarly journals Challenges of in vitro conservation of Сitrus germplasm resources

2019 ◽  
Vol 23 (1) ◽  
pp. 24-28
Author(s):  
V. M. Gorshkov ◽  
L. S. Samarina ◽  
R. V. Kulyan ◽  
V. I. Malyarovskaya ◽  
A. V. Ryndin ◽  
...  
Keyword(s):  
Photosynthetic Activity ◽  
Slow Growth ◽  
In Vitro Conservation ◽  
Growth Potential ◽  
Surface Sterilization ◽  
The Third ◽  
Citrus Varieties ◽  
Technical Solutions ◽  
High Degree

The main problems of establishment a slow growth in vitro collection of citrus and other tree crops cultivars are high degree of fungal contamination of bud explants and low growth potential of shoots. In this regard, the aim of current research is to assess the efficiency of decontamination procedure and the possibility of tissue culture initiation and slow growth conservation of valuable lemon cultivars. The best results of surface sterilization were obtained using immersion solutions of 0.3 % Veltolen – 25 minutes or 10 % Domestos – 25–30 minutes. In these treatments, 27.7–33.0 % of aseptic explants were obtained, respectively. However, after the third subculture, the yield of aseptic viable explants decreased till 10 % as a result of secondary contamination by endophytic fungi. The addition of biocide (“Gavrish”) in a nutrient medium at a concentration of 1 ml/l helped to increase the yield of aseptic viable explants till 50 %. However, after the third subculture the photosynthetic activity and the pigments content as well as growth rate decreased. Plants dropped yellowish leaves and eventually died. Thus, 37.35 % of plantlets survived after 8 months of conservation, and only 14.6 % survived after 10 months. Even after the third month of conservation significant decrease in the viability index and the coefficient of photosynthetic activity occurred in plants. Chlorophyll a in leaves decreased from 1.59 to 1.14 mg/g during 12 months in vitro conservation. The similar tendency observed on clorophyll b and carotenoids content. The experiments were carried out for 5 years using different lemon cultivars and other citrus varieties and cultivars. Thus, micropropagation and slow growth in vitro conservation of valuable lemon cultivars are still problematic and requires new technical solutions due to the low growth potential of plantlets raised from the mature buds that is consistent with the data of other researchers.

In Vitro Conservation ofTurbinicarpus(Cactaceae) Under Slow Growth Conditions

Haseltonia ◽  
2012 ◽  
Vol 17 ◽  
pp. 51-57 ◽  
Author(s):  
Eugenio Pérez-Molphe-Balch ◽  
Martha Evelia Pérez-Reyes ◽  
MA. De Lourdes De La Rosa-Carrillo
Keyword(s):  
Slow Growth ◽  
Growth Conditions ◽  
In Vitro Conservation
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