Members of the Kunitz-type protease inhibitor gene family of potato inhibit soluble tuber invertase in vitro

2002 ◽  
Vol 45 (2-4) ◽  
pp. 163-176 ◽  
Author(s):  
Heike Glaczinski ◽  
A. Heibges ◽  
F. Salamini ◽  
Christiane Gebhardt
Keyword(s):  
Protease Inhibitor ◽  
Gene Family ◽  
Kunitz Type ◽  
Kunitz Type Protease Inhibitor ◽  
Inhibitor Gene

scholarly journals Kunitz type protease inhibitor EgKI-1 from the canine tapeworm Echinococcus granulosus as a promising anti-cancer therapeutic

2018 ◽  
Author(s):  
Shiwanthi L Ranasinghe ◽  
Glen M Boyle ◽  
Katja Fischer ◽  
Jeremy Potriquet ◽  
Jason P Mulvenna ◽  
...  
Keyword(s):  
Cell Growth ◽  
Protease Inhibitor ◽  
Hydatid Cyst ◽  
Echinococcus Granulosus ◽  
Cancer Cell ◽  
Dependent Manner ◽  
Anti Cancer ◽  
Kunitz Type ◽  
Kunitz Type Protease Inhibitor

AbstractEgKI-1, a member of the Kunitz type protease inhibitor family, is highly expressed by the oncosphere of the canine tapeworm Echinococcus granulosus, the stage that is infectious to humans and ungulates, giving rise to a hydatid cyst localized to the liver and other organs. Larval protoscoleces, which develop within the hydatid cyst, have been shown to possess anti-cancer properties, although the precise molecules involved have not been identified. We show that recombinant EgKI-1 inhibits the growth and migration of a range of human cancers including breast, melanoma and cervical cancer cell lines in a dose-dependent manner in vitro without affecting normal cell growth. Furthermore, EgKI-1 treatment arrested the cancer cell growth by disrupting the cell cycle and induced apoptosis of cancer cells in vitro. An in vivo model of triple negative breast cancer (MDA-MB-231) in BALB/c nude mice showed significant tumor growth reduction in EgKI-1-treated mice compared with controls. These findings indicate that EgKI-1 shows promise for future development as an anti-cancer therapeutic.

scholarly journals Joannsin, a novel Kunitz-type FXa inhibitor from the venom of Prospirobolus joannsi

2017 ◽  
Vol 117 (06) ◽  
pp. 1031-1039 ◽  
Author(s):  
Ning Luan ◽  
Chunling Zhou ◽  
Pengpeng Li ◽  
Rose Ombati ◽  
Xiuwen Yan ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Factor Xa ◽  
Potential Candidate ◽  
Amino Acid Residues ◽  
Secretory Glands ◽  
Fxa Inhibitor ◽  
Kunitz Type ◽  
Kunitz Type Protease Inhibitor

SummaryThe repugnatorial glands of millipedes release various defensive chemical secretions. Although varieties of such defensive secretions have been studied, none of them is protein or peptide. Herein, a novel factor Xa (FXa) inhibitor named joannsin was identified and characterised from repugnatorial glands of Prospirobolus joannsi. Joannsin is composed of 72 amino acid residues including six cysteines, which form three intra-molecular disulfide bridges. It is a member of Kunitz-type protease inhibitor family, members of which are also found in the secretory glands of other arthropods. Recombinant joannsin exhibited remarkable inhibitory activity against trypsin and FXa with a Ki of 182.7 ± 14.6 and 29.5 ± 4.7 nM, respectively. Joannsin showed strong anti-thrombosis functions in vitro and in vivo. Joannsin is the first peptide component in millipede repugnatorial glands to be identified and is a potential candidate and/or template for the development of anti-thrombotic agents. These results also indicated that there is Kunitz-type protease inhibitor toxin in millipede repugnatorial glands as in other arthropods secretory glands.

PIVL, a snake venom Kunitz-type serine protease inhibitor, inhibits in vitro and in vivo angiogenesis

2014 ◽  
Vol 95 ◽  
pp. 149-156 ◽  
Author(s):  
Maram Morjen ◽  
Stéphane Honoré ◽  
Amine Bazaa ◽  
Zaineb Abdelkafi-Koubaa ◽  
Ameneallah Ellafi ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Serine Protease ◽  
Snake Venom ◽  
Serine Protease Inhibitor ◽  
Kunitz Type

scholarly journals Enhanced Plasmin Inhibition by a Reactive Center Lysine Mutant of the Kunitz-type Protease Inhibitor Domain of the Amyloid β-Protein Precursor

1995 ◽  
Vol 270 (39) ◽  
pp. 22827-22830 ◽  
Author(s):  
William E. Van Nostrand ◽  
Alvin H. Schmaier ◽  
Robert S. Siegel ◽  
Steven L. Wagner ◽  
William C. Raschke
Keyword(s):  
Protease Inhibitor ◽  
Amyloid Β ◽  
Amyloid Β Protein ◽  
Protein Precursor ◽  
Β Protein ◽  
Reactive Center ◽  
Kunitz Type ◽  
Kunitz Type Protease Inhibitor
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