The isolation, identification and quantitative determination of ethyl alcohol normally present in human and animal tissues

Mikrochemie ◽  
1932 ◽  
Vol 11 (1) ◽  
pp. 167-199 ◽  
Author(s):  
A. O. Gettler ◽  
J. B. Niederl ◽  
A. A. Benedetti-Pichler
Keyword(s):  
Quantitative Determination ◽  
Ethyl Alcohol ◽  
Animal Tissues

Determination of Penicillin G, Ampicillin, and Cephapirin Residues in Tissues

1983 ◽  
Vol 66 (1) ◽  
pp. 176-179
Author(s):  
Arnost B Vilim ◽  
Lyse Larocque
Keyword(s):  
Quantitative Determination ◽  
Bacillus Stearothermophilus ◽  
Residue Analysis ◽  
Penicillin G ◽  
Animal Tissues ◽  
Microbiological Method

Abstract A cylinder plate microbiological method was developed for the rapid, quantitative determination of penicillin G, ampicillin, and cephapirin in animal tissues. The method uses agar plates seeded with stable spores of Bacillus stearothermophilus var. calidolactis and incubated 4 h at 64°C. Standard curves were obtained for the following ranges of concentration of antibiotics in tissues: 0.02-0.32 IU penicillin G/g, 0.0125-0.2 μg ampicillin/g, and 0.02-0.32 μg cephapirin/g. The proposed method is suitable not only for penicillin residue analysis, for which the sensitivity has been greatly improved compared with the Sarcina lutea method, but also for depletion studies on these antibiotics, which are commonly used to treat diseases in food-producing animals.

Extraction, Cleanup, and Quantitative Determination of Aflatoxins B1 and M1 in Beef Liver

1979 ◽  
Vol 62 (5) ◽  
pp. 1080-1082
Author(s):  
Mary W Trucksess ◽  
Leonard Stoloff
Keyword(s):  
Citric Acid ◽  
Thin Layer ◽  
Quantitative Determination ◽  
Aflatoxin B1 ◽  
Aflatoxin M1 ◽  
Animal Tissues ◽  
Beef Liver ◽  
Layer Chromatography ◽  
Extracting Solvent

Abstract A method for the determination of aflatoxin Bx in eggs was applicable for aflatoxin B1 in liver, but ineffective for aflatoxin Mx in liver because of poor recovery of added aflatoxin and interferences in thin layer chromatography. The method was modified by the addition of citric acid to the extracting solvent and ammonium sulfate to the extract solution for removing protein. The elution system for silica gel column cleanup was also changed by substituting methanol for acetone, and adding a step for confirmation of aflatoxin M1 identity. The method has been used successfully for survey and research on aflatoxin residues in animal tissues.

scholarly journals Time-resolved fluoroimmunoassay for quantitative determination of tylosin and tilmicosin in edible animal tissues

2013 ◽  
Vol 58 (15) ◽  
pp. 1838-1842 ◽  
Author(s):  
Shu Wei ◽  
Tao Le ◽  
Yong Chen ◽  
Jian Xu ◽  
HongQiu He ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Animal Tissues ◽  
Time Resolved

Determination of Saccharin in Biological Materials by Gas-Liquid Chromatography

1971 ◽  
Vol 54 (5) ◽  
pp. 1140-1145
Author(s):  
Robert J Daun
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Quantitative Determination ◽  
Methyl Iodide ◽  
Chromatographic Separation ◽  
Gas Liquid Chromatography ◽  
Ionization Detector ◽  
Animal Tissues ◽  
Flame Ionization

Abstract A method is described for the quantitative determination of saccharin in urine, feces, blood, and animal tissues. The saccharin is extracted with diethyl ether and methylated with methyl iodide to provide a volatile derivative for gas-liquid chromatography. Higher levels, as found in urine and feces, are determined with a flame ionization detector and lower levels, as in blood and tissues, are analyzed with an electron capture detector after a thin layer chromatographic separation.

Colorimetric Determination of Aconitine in Galenicals and in Biological Samples

1976 ◽  
Vol 59 (4) ◽  
pp. 799-801
Author(s):  
Mohamed S Karawya ◽  
Abdel-Aziz A Sharaf ◽  
Aly M Diab
Keyword(s):  
Quantitative Determination ◽  
Biological Samples ◽  
Cobalt Complex ◽  
Colorimetric Determination ◽  
Animal Tissues ◽  
Sensitive Method

Abstract A sensitive method is presented for determining aconitine. Aconitine is complexed with Co2+, the aconitine-cobalt complex is extracted with chloroform, and the absorbance is measured at 320 nm. The sensitivity of the method ranged between 0.06 and 3 mg/25 ml, and the color was stable for 6 hr. The method was successfully applied for the quantitative determination of aconitine in animal tissues.

scholarly journals METHODS FOR QUANTITATIVE DETERMINATION OF TOTAL FLAVONOIDS IN QUERCUS ROBUR L. BUDS

2021 ◽  
Vol 9 (5) ◽  
pp. 356-366
Author(s):  
N. A. Ryabov ◽  
V. M. Ryzhov ◽  
V. A. Kurkin
Keyword(s):  
Medicinal Plant ◽  
Quantitative Determination ◽  
Ethyl Alcohol ◽  
Quercus Robur ◽  
Raw Materials ◽  
Uv Spectrophotometry ◽  
Total Flavonoids ◽  
Plant Raw Materials ◽  
Wide Range

Currently, the actual task of modern pharmacy is to study the chemical composition and pharmacological properties of plant objects. Within the framework of this concept, it seems interesting to study Quercus robur L. buds. One of the promising groups of biologically active compounds of Quercus robur L. buds are flavonoids. This group of substances has a wide range of a pharmacological activity, which is significant in the creation of new medicines based on medicinal plant raw materials.The aim of the article was to work out methods for quantitative determination of total flavonoids in Quercus robur l. buds.Materials and methods. The research materials were aqueous-alcoholic extracts from Quercus robur L. buds with 70% ethyl alcohol which were analyzed by differential UV spectrophotometry on spectrophotometer “SF 2000” (Russia).Results. The methods for quantitative determination of total flavonoids in Quercus robur L. buds by differential UV spectrophotometry, has been developed using a standard sample of cynaroside at the analytical wavelength of 400 nm. The optimum parameters for the extraction of total flavonoids from Quercus robur L. buds have been determined. They are: the optimum extractant is 70% ethyl alcohol; the “raw material-extractant” ratio is 1:50; the extraction time is 120 min, the degree of atomization is 2 mm.The content of total flavonoids for Quercus robur L. buds has been determined; it varies from 0.27%±0.01 to 0.44%±0.02. These results make possible to recommend the content of total flavonoids for this type of raw materials not less than 0.25% as a lower limit.Conclusion. The data obtained in the course of the experiment, makes it possible to conclude that a further study of Quercus robur L. buds is promising, and it also contributes to the implementation of medicinal plant raw materials “Quercus robur L. buds” in the State Pharmacopoeia (Russia).

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