Computation of the lateral and axial point spread functions in confocal imaging systems using binary amplitude mask

Pramana ◽  
2006 ◽  
Vol 66 (6) ◽  
pp. 1037-1048 ◽  
Author(s):  
A. M. Hamed
Keyword(s):  
Confocal Imaging ◽  
Imaging Systems ◽  
Point Spread Functions ◽  
Point Spread

Extending depth of field for hybrid imaging systems via the use of both dark and dot point spread functions

2016 ◽  
Vol 55 (26) ◽  
pp. 7345 ◽  
Author(s):  
L. V. Nhu ◽  
Zhigang Fan ◽  
Shouqian Chen ◽  
Fanyang Dang
Keyword(s):  
Hybrid Imaging ◽  
Imaging Systems ◽  
Depth Of Field ◽  
Point Spread Functions ◽  
Point Spread

scholarly journals Axial localization and tracking of self-interference nanoparticles by lateral point spread functions

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yongtao Liu ◽  
Zhiguang Zhou ◽  
Fan Wang ◽  
Günter Kewes ◽  
Shihui Wen ◽  
...  
Keyword(s):  
Mirror Image ◽  
Excitation Wavelength ◽  
Far Field ◽  
Point Spread Functions ◽  
Microscopy Imaging ◽  
Localization Accuracy ◽  
Field Patterns ◽  
Atomic Force ◽  
Point Spread ◽  
Sensing Technology

AbstractSub-diffraction limited localization of fluorescent emitters is a key goal of microscopy imaging. Here, we report that single upconversion nanoparticles, containing multiple emission centres with random orientations, can generate a series of unique, bright and position-sensitive patterns in the spatial domain when placed on top of a mirror. Supported by our numerical simulation, we attribute this effect to the sum of each single emitter’s interference with its own mirror image. As a result, this configuration generates a series of sophisticated far-field point spread functions (PSFs), e.g. in Gaussian, doughnut and archery target shapes, strongly dependent on the phase difference between the emitter and its image. In this way, the axial locations of nanoparticles are transferred into far-field patterns. We demonstrate a real-time distance sensing technology with a localization accuracy of 2.8 nm, according to the atomic force microscope (AFM) characterization values, smaller than 1/350 of the excitation wavelength.

scholarly journals Two simple criteria to estimate an objective’s performance when imaging in non design tissue clearing solutions

2019 ◽  
Author(s):  
Sabrina Asteriti ◽  
Valeria Ricci ◽  
Lorenzo Cangiano
Keyword(s):  
Image Quality ◽  
High Refractive Index ◽  
Optimal Conditions ◽  
Point Spread Functions ◽  
Tissue Clearing ◽  
Fluorescent Microscope ◽  
Immersion Medium ◽  
Quality Degradation ◽  
Point Spread ◽  
The Impact

ABSTRACTTissue clearing techniques are undergoing a renaissance motivated by the need to image fluorescence deep in biological samples without physical sectioning. Optical transparency is achieved by equilibrating tissues with high refractive index (RI) solutions, which require expensive optimized objectives to avoid aberrations. One may thus need to assess whether an available objective is suitable for a specific clearing solution, or the impact on imaging of small mismatches between cleared sample and objective design RIs. We derived closed form approximations for image quality degradation versus RI mismatch and other parameters available to the microscopist. We validated them with computed (and experimentally confirmed) aberrated point spread functions, and by imaging fluorescent neurons in high RI solutions. Crucially, we propose two simple numerical criteria to establish: (i) the degradation in image quality (brightness and resolution) from optimal conditions of any clearing solution/objective combination; (ii) which objective, among several, achieves the highest resolution in a given immersion medium. These criteria apply directly to the widefield fluorescent microscope but are also closely relevant to more advanced microscopes.

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