scholarly journals Overview literature on matrix assisted laser desorption ionization mass spectroscopy (MALDI MS): Basics and its applications in characterizing polymeric materials

2005 ◽  
Vol 28 (6) ◽  
pp. 515-528 ◽  
Author(s):  
R. N. Jagtap ◽  
A. H. Ambre
Keyword(s):  
Mass Spectroscopy ◽  
Polymeric Materials ◽  
Laser Desorption ◽  
Maldi Ms ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Ionization Mass Spectroscopy ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging

2019 ◽  
Vol 88 (4) ◽  
pp. 443-449
Author(s):  
Tomáš Komprda ◽  
Zbyšek Sládek ◽  
Veronika Švehlová ◽  
Zuzana Lacková ◽  
Roman Guráň ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mass Spectroscopy ◽  
Laser Desorption ◽  
Control Fish ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Ionization Mass Spectroscopy ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

The objective of the present study was to compare the effects of dietary oils containing polyunsaturated fatty acids (PUFA) n-3 and n-6, respectively, on cutaneous wound healing in rats, and to demonstrate the usefulness of the matrix-assisted-laser-desorption-ionization mass spectroscopy-imaging (MALDI MSI) method in this type of experiment. Superiority of PUFA n-3 in this context was the tested hypothesis. Four groups of male Wistar rats by twelve animals each were fed a diet with added 5% of palm oil (P; control), fish oil (F), Schizochytrium microalga oil (Sch) and safflower oil (S), respectively, for eight weeks. Consequently, dorsal full-thickness cutaneous excisions were performed, and selected markers of wound healing were evaluated 18 days post excision. The median of signal intensity corresponding to an amount of collagen α-1 (III) fragment, quantified using MALDI MSI, decreased in a sequence P > F > Sch > S (P < 0.001). Using haematoxylin-eosin staining of the histological preparations, semi-quantitatively assessed epithelium height tended to decrease in the order of P > S > Sch > F; the wound extent in the sequence of P > Sch > S > F; and the sequence of the progress of neo-angiogenesis was assessed as S > P > F ≈ Sch. It was concluded that the tested hypothesis was confirmed only partially: PUFA n-3 showed better results regarding the wound extent, but were inferior in terms of epithelium height and progress of neo-angiogenesis. This was the first time MALDI MSI was successfully employed for evaluating skin wound healing in a rat model.

scholarly journals Application of dyes as doping agents in MALDI-MS matrices for the signal enhancement of proteins

RSC Advances ◽  
2017 ◽  
Vol 7 (11) ◽  
pp. 6598-6604
Author(s):  
Arslan Ali ◽  
Najia Shahid ◽  
Syed Ghulam Musharraf
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Maldi Ms ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
As Doping ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Dyes can significantly influence the ionization of proteins, when used as dopants in conventional matrices used for Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS).

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