Effect of postweaning protein deficiency on the content and lipid composition of gray and white matter in neonatally undernourished rat brain

1982 ◽  
Vol 4 (4) ◽  
pp. 463-467
Author(s):  
T. Sanjeeva Reddy ◽  
C. V. Ramakrishnan
Keyword(s):  
White Matter ◽  
Rat Brain ◽  
Lipid Composition ◽  
Protein Deficiency

Lipid composition of gray and white matter in developing rat brain

1983 ◽  
Vol 1 (1) ◽  
pp. 65-74 ◽  
Author(s):  
T. Sanjeeva Reddy ◽  
R. Rajalakshmi ◽  
C. V. Ramakrishnan
Keyword(s):  
White Matter ◽  
Rat Brain ◽  
Lipid Composition ◽  
Developing Rat

scholarly journals Rapid Alterations in Cerebral White Matter Lipid Profiles After Ischemic-Reperfusion Brain Injury in Fetal Sheep as Demonstrated by MALDI-Mass Spectrometry

2019 ◽  
Vol 22 (4) ◽  
pp. 344-355 ◽  
Author(s):  
Gina M Gallucci ◽  
Ming Tong ◽  
Xiaodi Chen ◽  
Barbara S Stonestreet ◽  
Amy Lin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
White Matter ◽  
Lipid Composition ◽  
Lipid Profiles ◽  
Maldi Ms ◽  
Cerebral White Matter ◽  
Ionization Mass ◽  
Myelin Lipid ◽  
Fetal Sheep ◽  
Bilateral Carotid

Background Perinatal ischemia-reperfusion (I/R) injury of cerebral white matter causes long-term cognitive and motor disabilities in children. I/R damages or kills highly metabolic immature oligodendroglia via oxidative stress, excitotoxicity, inflammation, and mitochondrial dysfunction, impairing their capacity to generate and maintain mature myelin. However, the consequences of I/R on myelin lipid composition have not been characterized. Objective This study utilized matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to assess alterations in cerebral supraventricular white matter myelin lipid profiles in a fetal sheep model of perinatal I/R. Methods Fetal sheep (127 days gestation) were studied after 30 minutes of bilateral carotid artery occlusion followed by 4 (n = 5), 24 (n = 7), 48 (n = 3), or 72 (n = 5) hours of reperfusion, or sham treatment (n = 5). White matter lipids were analyzed by negative ion mode MALDI-MS. Results Striking I/R-associated shifts in phospholipid and sphingolipid expression occurred over the 72-hour time course with most responses detected within 4 hours of reperfusion and progressing at the 48- and 72-hour points. I/R decreased expression of phosphatidic acid and phosphatidylethanol amine and increased phosphatidylinositol, sulfatide, and lactosylceramide. Conclusions Cerebral I/R in mid-gestation fetal sheep causes rapid shifts in white matter myelin lipid composition that may reflect injury, proliferation, or recovery of immature oligodendroglia.

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