Determination of kinetic parameters of epitope-paratope interaction based on solid phase binding: An inexpensive alternate to biospecific interaction analysis

1996 ◽  
Vol 21 (5) ◽  
pp. 641-651 ◽  
Author(s):  
G. S. Murthy ◽  
N. Venkatesh
Keyword(s):  
Kinetic Parameters ◽  
Solid Phase ◽  
Interaction Analysis ◽  
Solid Phase Binding ◽  
Biospecific Interaction

One-step assay for the determination of free protein S antigen in plasma using real-time biospecific interaction analysis

1998 ◽  
Vol 9 (4) ◽  
pp. 333-342 ◽  
Author(s):  
C. Ravanat ◽  
M-L. Wiesel ◽  
S. Schuhler ◽  
J. Dambach ◽  
J. Amiral ◽  
...  
Keyword(s):  
Real Time ◽  
Interaction Analysis ◽  
Protein S ◽  
Free Protein ◽  
One Step ◽  
Biospecific Interaction

scholarly journals Determination of kinetic parameters of the reaction of formation of the solid-phase adsorbents

2015 ◽  
Vol 3 (4(23)) ◽  
pp. 38
Author(s):  
Виктор Иванович Булавин ◽  
Николай Андреевич Блинков
Keyword(s):  
Kinetic Parameters ◽  
Solid Phase

MEASUREMENT OF STEROID BINDING PROTEINS

1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S104-S121 ◽  
Author(s):  
E. E. Baulieu ◽  
J. P. Raynaud ◽  
E. Milgrom
Keyword(s):  
Kinetic Parameters ◽  
Binding Proteins ◽  
Binding Specificity ◽  
Binding Parameters ◽  
Target Organs ◽  
Biological Mixtures ◽  
Steroid Binding Proteins ◽  
Steroid Binding ◽  
Steroid Binding Protein

ABSTRACT A brief review of the characteristics of steroid binding proteins found in the plasma and in some target organs is presented, followed by some general remarks on binding »specificity« and binding parameters. Useful techniques for measuring binding parameters at equilibrium are reported, both those which keep the equilibrium intact and those which implicate its disruption. A concept is developed according to which the determination of a specific steroid binding protein is based on the »differential dissociation« of the several steroid binding complexes present in most biological mixtures. Methods which allow determination of the kinetic parameters of the binding systems are also presented. Various representations of the binding and therefore different modes of graphic representation and calculation are discussed, including the recent »proportion graph« method.

Modern determinants of endometrium hyperplastic processes pathogenesis

2016 ◽  
pp. 137-142
Author(s):  
V.O. Benyuk ◽  
◽  
V.M. Goncharenko ◽  
T.R. Nykoniuk ◽  
◽  
...  
Keyword(s):  
Conservative Therapy ◽  
Solid Phase ◽  
Research Direction ◽  
Immunohistochemical Method ◽  
Results Of Treatment ◽  
Treatment And Prevention ◽  
Receptor Phenotype ◽  
The Relationship ◽  
Endometrial Proliferation

The objective: to еxplore the relationship between the activity of endometrial proliferation and the state of the local immune response in the uterus in the conditions berprestasi process. Patients and methods. Examined 228 women of reproductive and perimenopausal age with endometrial pathology using ultrasound and then performing hysteroresectoscopy. Determination of the concentrations of the cytokines IL-1, IL-2, IL-6 and TNF was performed by solid phase ELISA. Results. Found a trend that confirms the loss of sensitivity to hormones at the stage of malignancy of the endometrium and can be used as diagnostic determinants in determining the nature of intrauterine pathology and criterion of the effectiveness of conservative therapy. Conclusion. Improving etiopatogenetice approach to the therapy of hyperplastic proce.sses of endometrium with determination of receptor phenotype of the endometrium is a research direction in modern gynecology, which will help to improve the results of treatment and prevention of intrauterine pathology. Key words: endometrial hyperplasia,the receptors for progesterone and estrogen, immunohistochemical method.

Laboratory Determination of the Solid Phase Composition of Technogenically Salinized Peatlands: Possibilities and Limitations

2020 ◽  
Vol 75 (3) ◽  
pp. 131-137
Author(s):  
Yu. N. Vodyanitskii ◽  
N. A. Avetov ◽  
A. T. Savichev ◽  
S. Ya. Trofimov ◽  
E. A. Shishkonakova
Keyword(s):  
Phase Composition ◽  
Solid Phase ◽  
Laboratory Determination ◽  
Solid Phase Composition
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