Expression of parasporal crystal protein (δ—endotoxin) gene(s) ofBacillus thuringiensis var.israelensis in sporogenic and asporogenic mutant strains ofBacillus cereus

1994 ◽  
Vol 19 (2) ◽  
pp. 145-153
Author(s):  
Pramatha R Bhattacharya
Keyword(s):  
Crystal Protein ◽  
Parasporal Crystal ◽  
Ofbacillus Thuringiensis ◽  
Mutant Strains ◽  
Parasporal Crystal Protein ◽  
Ofbacillus Cereus

scholarly journals Dose Response of Omnivorous Leafroller to Commercial Formulations of Bacillus Thuringiensis Var. Kurstaki in the Laboratory, 1992

1994 ◽  
Vol 19 (1) ◽  
pp. 370-370
Author(s):  
J. Steven Tebbets ◽  
Patrick V. Vail
Keyword(s):  
Dose Response ◽  
Crystal Protein ◽  
Protein Bodies ◽  
Bacterial Cells ◽  
Parasporal Crystal ◽  
First Instar ◽  
Parasporal Crystal Protein ◽  
Live Bacteria ◽  
Omnivorous Leafroller ◽  
Feeding Trials

Abstract We compared three formulations of B. thuringiensis var. kurstaki (Btk) for their insecticidal activity against first-instar OLR. Products tested included: (1) NOVO Laboratories Biobit, a flowable concentrate containing live bacteria with spores and parasporal crystal protein bodies; (2) Abbott Laboratories Dipel 2X, a wettable powder also containing active bacteria with spores and parasporal crystal protein bodies; and (3) Mycogen Corporations MVP Bioinsecticide, a flowable concentrate containing dead bacterial cells which bioencapsulate the Btk proteins. The AI in each of these products is the insecticidal protein, or delta-endotoxin, of Btk. Dose-response was determined by feeding trials exposing first-instar OLR to each product. Serial dilutions of each formulation were made and 10 μ1 of each dilution was applied to the surface of an agar-based diet (Bioserv No. 9370) and then allowed to air dry. One larva was placed in each vial containing ca. 1 ml of surface-contaminated diet (surface area = 1 cm2). Thirty to 60 larvae were observed for each dilution and formulation tested. Doses were expressed in mg AI per sq. cm. Mortality evaluations were made after 3, 5, and 7 days. Response data were analyzed by the POLO-PC PROBIT procedure (LeOra Software, 1987).

Bacillus thuringiensiscrystal protein toxicity against plant-parasitic nematodes

2008 ◽  
Vol 5 (1) ◽  
pp. 13-17 ◽  
Author(s):  
Yu Zi-Quan ◽  
Wang Qian-Lan ◽  
Liu Bin ◽  
Zou Xue ◽  
Yu Zi-Niu ◽  
...  
Keyword(s):  
Parasitic Nematodes ◽  
Meloidogyne Hapla ◽  
Plant Parasitic Nematodes ◽  
Parasporal Crystal ◽  
Crystal Proteins ◽  
Parasporal Crystal Protein ◽  
Pratylenchus Scribneri ◽  
Ditylenchus Destructor ◽  
Protein Toxicity ◽  
Plant Parasitic

AbstractA bioassay method was developed to use the parasporal crystal protein ofBacillus thuringiensisagainst plant-parasitic nematodes. Using this method, the parasporal crystal proteins of tenBtstrains showed activity against plant-parasitic nematodes. The toxicity of YBT-021 againstMeloidogyne hapla,Pratylenchus scribneri,Tylenchorhynchussp.,Ditylenchus destructorandAphelenchoidessp. was also assayed. The resulting LC50values were 35.62 μg/ml, 75.65 μg/ml, 94.31 μg/ml, 215.21 μg/ml and 128.76 μg/ml, respectively.

scholarly journals Chemical and Morphological Studies of Bacterial Spore Formation

1959 ◽  
Vol 6 (3) ◽  
pp. 499-506 ◽  
Author(s):  
I. Elizabeth Young ◽  
Philip C. Fitz-James
Keyword(s):  
Cell Proliferation ◽  
Protein Synthesis ◽  
Nucleic Acid ◽  
Cell Growth ◽  
Crystal Protein ◽  
Nucleic Acid Content ◽  
Crystal Formation ◽  
Parasporal Crystal ◽  
Purine Analogue ◽  
Morphological Studies

The purine analogue, 8-azaguanine, was added to cultures of the parasporal crystal-forming organism Bacillus cereus var. alesti at different times during growth and synchronous sporulation. The effect of its incorporation has been studied with particular reference to cell growth, nucleic acid composition, cytology, and the synthesis of the spore and crystal protein. Additions of the analogue during any stage of growth prevented further cell proliferation and all spore and crystal formation. Since both nucleic acids continued to be formed, cells of an increased size developed, containing large masses of chromatin in the form of condensed balls or axial cords. Lipid-containing inclusions also appeared following these additions and were usually aggregated at the centre or poles of the cells. The analogue could be isolated as the ribonucleotide from both the acid soluble and RNA fractions of these inhibited cells. Additions of the analogue following commencement of sporulation did not prevent either spore or crystal formation or affect the nucleic acid content of the sporulating cells. However, as before, the 8-azaguanine was incorporated into both the acid soluble and RNA of the cells, but not into these fractions of the spores ultimately formed. The implications of these findings are discussed in relation to crystal protein synthesis.

A Toxic Fragment from the Entomocidal Crystal Protein ofBacillus thuringiensis

1984 ◽  
Vol 48 (3) ◽  
pp. 611-619
Author(s):  
Yasunori Nagamatsu ◽  
Yuichi Itai ◽  
Chitoshi Hatanaka ◽  
Gunki Funatsu ◽  
Katsuya Hayashi
Keyword(s):  
Crystal Protein ◽  
Ofbacillus Thuringiensis
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