Role of heme in mitochondrial biogenesis: Transcriptional and post-transcriptional regulation of the expression of Iso-I-cytochrome C gene during glucose repression-derepression in cells ofSaccharomyces cerevisiae

1990 ◽  
Vol 15 (4) ◽  
pp. 329-339
Author(s):  
K. S. K. Balaji ◽  
G. Gopalan ◽  
C. Rajamanickam
Keyword(s):  
Transcriptional Regulation ◽  
Cytochrome C ◽  
Mitochondrial Biogenesis ◽  
Glucose Repression ◽  
Post Transcriptional Regulation ◽  
In Cells

scholarly journals Fast and Efficient 5′P Degradome Library Preparation for Analysis of Co-Translational Decay in Arabidopsis

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 466
Author(s):  
Marie-Christine Carpentier ◽  
Cécile Bousquet-Antonelli ◽  
Rémy Merret
Keyword(s):  
Gene Expression ◽  
Quality Control ◽  
Transcriptional Regulation ◽  
High Throughput ◽  
Library Preparation ◽  
Working Day ◽  
Set Up ◽  
Post Transcriptional Regulation ◽  
Commercial Kit

The recent development of high-throughput technologies based on RNA sequencing has allowed a better description of the role of post-transcriptional regulation in gene expression. In particular, the development of degradome approaches based on the capture of 5′monophosphate decay intermediates allows the discovery of a new decay pathway called co-translational mRNA decay. Thanks to these approaches, ribosome dynamics could now be revealed by analysis of 5′P reads accumulation. However, library preparation could be difficult to set-up for non-specialists. Here, we present a fast and efficient 5′P degradome library preparation for Arabidopsis samples. Our protocol was designed without commercial kit and gel purification and can be easily done in one working day. We demonstrated the robustness and the reproducibility of our protocol. Finally, we present the bioinformatic reads-outs necessary to assess library quality control.

Post-transcriptional regulation of bovine parathyroid hormone synthesis

1993 ◽  
Vol 10 (1) ◽  
pp. 43-49 ◽  
Author(s):  
N S Hawa ◽  
J L H O'Riordan ◽  
S M Farrow
Keyword(s):  
Transcriptional Regulation ◽  
Steady State ◽  
Actinomycin D ◽  
Mrna Levels ◽  
Dot Blot ◽  
Hormone Synthesis ◽  
Membrane Bound ◽  
Post Transcriptional Regulation ◽  
Bovine Parathyroid Hormone ◽  
In Cells

ABSTRACT Incubation of bovine parathyroid cells for 48 h in 0·4 mmol calcium/l had no significant effect on steady-state preproparathyroid hormone (preproPTH) mRNA levels when compared with cells incubated in 1·0 mmol calcium/l, but low calcium concentrations increased the membrane-bound polysomal content of preproPTH mRNA by 200±16% (mean±s.d.). No preproPTH mRNA was detected on free polysomes. Actinomycin D (5 and 10 μg/ml) had no effect on steady-state preproPTH mRNA levels measured in dot-blot assays after 24 h, but reduced levels in cells incubated in 1·0 mmol calcium/l to 54±16% and 39±12% of control values respectively after 48 h of incubation. Similarly, in cells incubated in 0·4 mmol calcium/l, actinomycin D (5 and 10μg/ml) reduced steady-state preproPTH mRNA levels to 57±13% and 45±5% of control values respectively. Actinomycin D did not prevent the rise in polysomal content of preproPTH mRNA induced in cells by incubation in 0·4 mmol calcium/l, but increased polysomal content in cells incubated in 0·4 and 1·0mmol calcium/l by 159±9% and 164±13% respectively after 48 h. These results demonstrate post-transcriptional regulation of PTH synthesis in cultured bovine parathyroid cells, and suggest that this control involves a protein which may be calcium-sensitive.

scholarly journals Role of N6-Methyladenosine RNA Modification in Cardiovascular Disease

2021 ◽  
Vol 8 ◽  
Author(s):  
Dandan Song ◽  
Jianhua Hou ◽  
Junduo Wu ◽  
Junnan Wang
Keyword(s):  
Risk Factors ◽  
Cardiovascular Disease ◽  
Transcriptional Regulation ◽  
Recent Progress ◽  
Rna Modification ◽  
Non Coding Rnas ◽  
Post Transcriptional Regulation

Despite treatments being improved and many risk factors being identified, cardiovascular disease (CVD) is still a leading cause of mortality and disability worldwide. N6-methyladenosine (m6A) is the most common, abundant, and conserved internal modification in RNAs and plays an important role in the development of CVD. Many studies have shown that aabnormal m6A modifications of coding RNAs are involved in the development of CVD. In addition, non-coding RNAs (ncRNAs) exert post-transcriptional regulation in many diseases including CVD. Although ncRNAs have also been found to be modified by m6A, the studies on m6A modifications of ncRNAs in CVD are currently lacking. In this review, we summarized the recent progress in understanding m6A modifications in the context of coding RNAs and ncRNAs, as well as their regulatory roles in CVD.

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