Quantitative reversed phase HPLC analysis of L-ascorbic acid (Vitamin C) and identification of its degradation products

1987 ◽  
Vol 24 (1) ◽  
pp. 371-376 ◽  
Author(s):  
Linda L. Lloyd ◽  
F. P. Warner ◽  
C. A. White ◽  
J. F. Kennedy
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Hplc Analysis ◽  
Degradation Products ◽  
Reversed Phase ◽  
Reversed Phase Hplc

Radiolytic degradation of methoxychlor in methanol and monitoring of radiolytic products by HPLC and GC-MS

2010 ◽  
Vol 98 (5) ◽  
Author(s):  
Shahid Bilal Butt ◽  
Muhammad Riaz
Keyword(s):  
Radiation Dose ◽  
Gamma Radiation ◽  
Degradation Products ◽  
Organic Pollutant ◽  
Reversed Phase ◽  
Methanol Solution ◽  
Reversed Phase Hplc ◽  
Experimental Conditions ◽  
Radiolytic Degradation ◽  
Gamma Radiation Dose

AbstractDegradation of priority organic pollutant methoxychlor in methanol solution by gamma irradiation under varied experimental conditions has been optimized. The solution of methoxychlor was air saturated before irradiation. The extent of radiolytic degradation efficiency was monitored by reversed phase HPLC-UV; two major and two minor degradation products were detected. For 5 kGy gamma radiation dose at a rate of 200 kGy h

scholarly journals Determination of Degradation Products of Doxercalciferol by Solid-Phase Extraction and Reversed-Phase HPLC

2013 ◽  
Vol 52 (6) ◽  
pp. 520-525
Author(s):  
N. Simonzadeh ◽  
B. Ronsen ◽  
S. Upadhyaya ◽  
E. Wilkinson ◽  
K. Kanesvaran ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Degradation Products ◽  
Reversed Phase ◽  
Phase Extraction ◽  
Reversed Phase Hplc

scholarly journals Simultaneous Identification of Phenolic Compound from the Honey of Stingless Bee by Using HPLC

2019 ◽  
Vol 5 ◽  
pp. 1
Author(s):  
Asma Nisar ◽  
Awang Bono ◽  
Hina Ahmad ◽  
Ambreen Lateef ◽  
Maham Mushtaq ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Gallic Acid ◽  
Royal Jelly ◽  
Antioxidant Properties ◽  
Reversed Phase ◽  
Hplc Method ◽  
Stingless Bee ◽  
Reversed Phase Hplc ◽  
Therapeutic Uses ◽  
Simultaneous Identification

Apitherapy is a branch of alternative medicine that uses honey bee products including honey, propolis, pollen, bee venom, and royal jelly. Stingless bee honey reportedly has many medicinal and therapeutic uses and excellent potency. This study aimed to identify the phenolic compounds using a fast and specific reversed-phase HPLC method in the extract of stingless bee honey. A magnetic stirrer was used for the pretreatment process of a sample with methanol at a temperature of 50°C for 40 min, followed by separation on a column size of 250 mm x 4.6 mm (5μm) hypersil gold-C18 (Thermo Electron Corporation) with water–methanol–acetonitrile (45:40:15 v/v/v) containing acetic acid 1.0% as a mobile phase. A 254-nm wavelength was used to detect the extract. The standard retention times of the gallic acid, rutin, ascorbic acid, quercetin, and kaempferol were found to be at 2.610, 2.875, 3.150, 5.789, and 8.983 min, respectively. The existence of gallic acid, rutin, ascorbic acid, kaempferol, and quercetin in the stingless bee honey extract was found to match according to the standard retention time. In the stingless bee honey, the retention times of gallic acid, rutin, ascorbic acid, quercetin, and kaempferol were found to be at 2.613, 2.866, 3.157, 5.790, and 8.966 min, respectively. In the stingless bee honey, the amounts of gallic acid, rutin, ascorbic acid, quercetin, and kaempferol were 1.426%, 2.533%, 16.922%, 1.851%, and 13.773%, respectively. According to the results, it is concluded that stingless bee honey is rich in phenolic acids and flavonoid compounds that have strong antioxidant properties.

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