High performance liquid chromatography analyses of emulsifiers: I. Quantitative determinations of mono- and diacylglycerols of saturated fatty acids

1978 ◽  
Vol 55 (9) ◽  
pp. 649-652 ◽  
Author(s):  
T. Riisom ◽  
L. Hoffmeyer
Keyword(s):  
Fatty Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Saturated Fatty Acids

Changes of dolichol and dolichyl fatty acyl esters during the germination and development of soybeans

1992 ◽  
Vol 70 (6) ◽  
pp. 466-469 ◽  
Author(s):  
Masataka Ishinaga ◽  
Toshimi Yamauchi ◽  
Keiko Egusa ◽  
Keiko Kuroda
Keyword(s):  
Fatty Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Saturated Fatty Acids ◽  
Behenic Acid ◽  
Fatty Acyl ◽  
Short Chain ◽  
Soybean Seedlings ◽  
Long Chain

The amounts of dolichol and dolichyl fatty acyl esters and their composition in various parts of soybean seedlings were determined during germination and development. The dolichol content of cotyledons decreased during germination. Dolichyl fatty acyl esters were identified in cotyledons and the amount was estimated by high performance liquid chromatography. The relative amounts of short-chain dolichols of 15, 16, and 17 isoprene units increased during development of the seedlings. The homologue distribution of free dolichol was different from that of dolichyl fatty acyl esters. The relative amounts of dolichols with 16, 17, and 18 isoprene units were greater in free dolichol than in dolichyl fatty acyl esters. The percentages of long-chain saturated fatty acids in dolichyl fatty acyl esters, specifically 21:0, 22:0, 23:0, 24:0, and 25:0, increased during development. These fatty acids represented more than 40% of the fatty acids in dolichyl fatty acyl esters in stems. These results suggest that dolichyl fatty acyl esters are not a storage form of dolichol. The large accumulation of dolichol and dolichyl fatty acyl esters in the leaves, where photosynthesis takes place, suggests some other function.Key words: dolichol, dolichyl fatty acy ester, soybean, behenic acid, lignoceric acid.

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