Nasal administration of arthritis-related T cell epitopes of heat shock protein 60 as a promising way for immunotherapy in chronic arthritis

Biotherapy ◽  
1998 ◽  
Vol 10 (3) ◽  
pp. 205-211 ◽  
Author(s):  
Berent Prakken ◽  
Marca Wauben ◽  
Peter van Kooten ◽  
Steve Anderton ◽  
Ruurd van der Zee ◽  
...  
Keyword(s):  
T Cell ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Chronic Arthritis ◽  
Nasal Administration ◽  
Heat Shock Protein 60 ◽  
T Cell Epitopes

Tolerogenic immune responses to novel T-cell epitopes from heat-shock protein 60 in juvenile idiopathic arthritis

The Lancet ◽  
2005 ◽  
Vol 366 (9479) ◽  
pp. 50-56 ◽  
Author(s):  
Sylvia Kamphuis ◽  
Wietse Kuis ◽  
Wilco de Jager ◽  
Gijs Teklenburg ◽  
Margherita Massa ◽  
...  
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
T Cell ◽  
Heat Shock ◽  
Immune Responses ◽  
Heat Shock Protein ◽  
Heat Shock Protein 60 ◽  
T Cell Epitopes

Heat shock protein T-cell epitopes as immunogenic carriers in subunit vaccines

Peptides 1994 ◽  
1995 ◽  
pp. 841-842 ◽  
Author(s):  
R. van der Zee ◽  
S. M. Anderton ◽  
C. A. F. Buskens ◽  
E. Alonso de Velasco ◽  
W. van Eden
Keyword(s):  
T Cell ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
T Cell Epitopes ◽  
Subunit Vaccines

Epitope Mapping of Porphyromonas gingivalis Heat-shock Protein and Human Heat-shock Protein in Human Atherosclerosis

2004 ◽  
Vol 83 (12) ◽  
pp. 936-940 ◽  
Author(s):  
J.-I. Choi ◽  
S.-W. Chung ◽  
H.-S. Kang ◽  
B.Y. Rhim ◽  
Y.-M. Park ◽  
...  
Keyword(s):  
T Cell ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
B Cell ◽  
Cell Epitope ◽  
T Cell Epitopes ◽  
Igg Antibody ◽  
B Cell Epitopes ◽  
Human Heat Shock Protein ◽  
Human Hsp60

To identify T- and/or cross-reactive B-cell epitopes of P. gingivalis and human heat-shock protein (HSP)60 in atherosclerosis patients, we synthesized 104 overlapping synthetic peptides spanning whole molecules of P. gingivalis HSP60 and human HSP60, respectively. T-cell epitopes of P. gingivalis HSP were identified with the use of previously established P. gingivalis HSP-reactive T-cell lines. B-cell epitopes of P. gingivalis HSP60 and human HSP60 were identified by the use of patients’ sera. Anti- P. gingivalis, anti- P. gingivalis HSP60, or anti-human HSP60 IgG antibody titers were higher in the atherosclerosis patients compared with the healthy subjects. Five immunodominant peptides of P. gingivalis HSP60, identified as T-cell epitopes, were also found to be B-cell epitopes. Moreover, 6 cross-reactive B-cell epitopes of human HSP60 were identified. It was concluded that P. gingivalis HSP60 might be involved in the immunoregulatory process of atherosclerosis, with common T- and/or B-cell epitope specificities and with cross-reactivity with human HSP60.

scholarly journals Immunization with a Peptide Corresponding to Chlamydial Heat Shock Protein 60 Increases the Humoral Immune Response in C3H Mice to a Peptide Representing Variable Domain 4 of the Major Outer Membrane Protein of Chlamydia trachomatis

1999 ◽  
Vol 6 (3) ◽  
pp. 356-363 ◽  
Author(s):  
Vladimir L. Motin ◽  
Luis M. de la Maza ◽  
Ellena M. Peterson
Keyword(s):  
Immune Response ◽  
Cell Proliferation ◽  
T Cell ◽  
Heat Shock ◽  
Chlamydia Trachomatis ◽  
Membrane Protein ◽  
Heat Shock Protein ◽  
T Cell Proliferation ◽  
Heat Shock Protein 60 ◽  
C3h Mice

ABSTRACT C3H (H-2k ) mice are susceptible to a vaginal challenge with human strains of Chlamydia trachomatis and thus are a useful strain for testing potentialChlamydia vaccine candidates. However, C3H mice are fairly poor responders in terms of the level of antibody resulting from immunization with potential protective peptides representing variable domains (VDs) of the major outer membrane protein (MOMP). C57BL/6 (H-2b ) mice, on the other hand, are moderately resistant to a vaginal challenge but are good responders to the chlamydial MOMP VDs. Peptides representing universal T-cell helper epitopes were employed to determine whether the antibody response to a peptide representing VD4 of the MOMP, which has been shown to contain neutralizing epitopes, could be enhanced in C3H and C57 mice. Universal T-cell helper peptides from tetanus toxin, the pre-S2 region of hepatitis B virus, and the mouse heat shock protein 60, as well as the corresponding segment of the Chlamydia heat shock protein 60 (hspct), were coadministered with the VD4 peptide. Peptides were coencapsulated in liposomes containing the adjuvant monophosphoryl lipid A and administered by using a combination of mucosal and intramuscular injection. The only T-cell helper peptide that improved the immune response as judged by antibody level, in vitro neutralization assays, and T-cell proliferation was hspct. The response in the C57BL/6 strain was not significantly enhanced with hspct over levels achieved with VD4 alone; however, in C3H mice the levels of serum antibody to C. trachomatisincreased to that seen in C57 mice. However, the molecular specificity and immunoglobulin subclass distribution differed from those of the C57 response, and the neutralizing titers and T-cell proliferation responses were lower. In both strains of mice, titers of vaginal antibody to C. trachomatis were low. In summary, of the T-helper peptides used, only hspct significantly enhanced the immune response of C3H mice to the VD4 peptide, but it had only a modest effect on the immune response of C57 mice.

scholarly journals HLA‐DR4‐restricted T‐cell epitopes from the mycobacterial 60 000 MW heat shock protein (hsp 60) do not map to the sequence homology regions with the human hsp 60

Immunology ◽  
1996 ◽  
Vol 87 (3) ◽  
pp. 421-427 ◽  
Author(s):  
A. S. MUSTAFA ◽  
K. E. A. LUNDIN ◽  
R. H. MELOEN ◽  
T. M. SHINNICK ◽  
A. F. W. COULSON ◽  
...  
Keyword(s):  
T Cell ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Sequence Homology ◽  
T Cell Epitopes
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