A simple and rapid colorimetric method for determination of free fatty acids for lipase assay

1986 ◽  
Vol 63 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Dae Y. Kwon ◽  
Joon S. Rhee
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Colorimetric Method

A New Colorimetric Method for the Determination of Free Fatty Acids with Acyl-CoA Synthetase and Acyl-CoA Oxidase1

1981 ◽  
Vol 89 (6) ◽  
pp. 1799-1803 ◽  
Author(s):  
Kohei HOSAKA ◽  
Toshiro KIKUCHI ◽  
Noboru MITSUHIDA ◽  
Akihiko KAWAGUCHI
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Colorimetric Method

Colorimetric Microdetermination of Free Fatty Acids

1973 ◽  
Vol 19 (4) ◽  
pp. 419-424 ◽  
Author(s):  
Felix G Soloni ◽  
Laura C Sardina
Keyword(s):  
Fatty Acids ◽  
Oxalic Acid ◽  
Free Fatty Acids ◽  
Filter Paper ◽  
Normal Values ◽  
Colorimetric Method ◽  
Reference Method ◽  
Chloroform Extract ◽  
Related Difference

Abstract A colorimetric method for determination of free fatty acids has been developed, based on the estimation of copper in a chloroform extract of their cupric salts with oxalic acid bis-(cyclohexylidenehydrazide). Contamination with inorganic copper during extraction is practically eliminated by using filter-paper pads. Specificity and interferences are discussed. Recoveries average 101.1%. Normal values are 10.0 ± 8.2 mg/dl (0.390 ± 0.320 mmol/liter), with no statistically significant sex-related difference. Correlation with the reference method of Dole and Meinertz [J. Biol. Chem. 235, 2595 (1960)] is 0.954. Ten samples can be measured in about 45 min.

Automated Colorimetric Determination of Free Fatty Acids in Biologic Fluids

1967 ◽  
Vol 13 (9) ◽  
pp. 744-751 ◽  
Author(s):  
C Dalton ◽  
C Kowalski
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Colorimetric Method ◽  
Extraction Procedure ◽  
Automated Analysis ◽  
Colorimetric Determination ◽  
Direct Extraction

Abstract For the routine determination of free fatty acids (FFA) the authors recommend a modification of the automated colorimetric method of Antonis (1) using the extraction procedure of ltaya and Ui (2). This direct extraction and automated analysis shows good correlation with Trout's (3) modification of Dole's (4) titrimetric procedure.

Enzymic determination of free fatty acids in serum.

1980 ◽  
Vol 26 (11) ◽  
pp. 1540-1543 ◽  
Author(s):  
H Okabe ◽  
Y Uji ◽  
K Nagashima ◽  
A Noma
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Colorimetric Method ◽  
Simultaneous Production ◽  
Production Of Hydrogen ◽  
Standard Solution ◽  
Acyl Coa Oxidase ◽  
Assay Precision ◽  
Mean Concentration

Abstract We describe the enzymic determination of free fatty acids in serum with use of acyl-CoA synthetase (EC l.2.1.3), acyl-CoA oxidase (no EC no. assigned) and peroxidase (EC 1.11.1.7). The free fatty acids are activated by acyl-CoA synthetase in the presence of ATP and coenzyme A. The acyl-CoA formed is oxidized by acyl-CoA oxidase to enoyl-CoA with simultaneous production of hydrogen peroxide, which is oxidatively coupled with 4-amino-antipyrine and 2,4-dibromphenol in the presence of peroxidase to yield a product that absorbs maximally at 505 nm. Standard curves prepared with various kinds of fatty acids are linear to at least 2.0 mmol/L and are practically congruent. Ascorbic acid or bilirubin interferes slightly. Results by the present method correlate well with those by the colorimetric method involving 2-(2-thiozolylazo)-p-cresol (r = 0.98). Replicate analyses of standard solution and of two kinds of control sera demonstrated the following between-assay precision: mean absorbance 0.224 (SD 0.002), CV 0.85%; mean concentration, 326 (SD 7.3) and 1076 (SD 22.7) mumol/L, CV 2.24 and 2.11%, respectively.

Enzymatic determination of serum-free fatty acids: A colorimetric method

1980 ◽  
Vol 107 (1) ◽  
pp. 193-198 ◽  
Author(s):  
Sakayu Shimizu ◽  
Yoshiki Tani ◽  
Hideaki Yamada ◽  
Masayoshi Tabata ◽  
Takashi Murachi
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Colorimetric Method ◽  
Serum Free Fatty Acids ◽  
Serum Free ◽  
Enzymatic Determination
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