Rapid CsCl gradients in fixed angle rotors for purification of viruses and organelle DNA

1983 ◽  
Vol 1 (3) ◽  
pp. 131-136 ◽  
Author(s):  
Laurence K. Grill ◽  
O. Mitch Griffith ◽  
Stephen J. Garger
Keyword(s):  
Fixed Angle ◽  
Organelle Dna

scholarly journals Minimising the risk of extensor pollicis longus rupture following volar plate fixation of distal radius fractures: a cadaveric study

2011 ◽  
Vol 93 (1) ◽  
pp. 57-60 ◽  
Author(s):  
DC Perry ◽  
DMG Machin ◽  
JA Casaletto ◽  
DJ Brown
Keyword(s):  
High Risk ◽  
Distal Radius ◽  
Plate Fixation ◽  
Tendon Injury ◽  
Distal Radius Fractures ◽  
Volar Plate ◽  
Radius Fractures ◽  
Extensor Pollicis Longus ◽  
Fixed Angle ◽  
Volar Plate Fixation

INTRODUCTION Rupture of extensor pollicis longus (EPL) tendon is a recognised complication following volar plate fixation of distal radius fractures, usually from attrition caused by prominent screws. We sought to identify the screw holes in some of the most commonly used plates which may precipitate tendon injury. SUBJECTS AND METHODS Three fixed-angle volar locking plates were sequentially positioned into 18 cadaveric arms. A wire was passed through each of the holes in the plates using a locking guide and the dorsal relationships noted. RESULTS Each plate had specific ‘high-risk’ holes which directed the wire towards the EPL groove. The central screw holes appeared mostly implicated in EPL injury. CONCLUSIONS Awareness of ‘high-risk’ holes and appropriate minor alterations in surgical technique may consequently decrease the incidence of EPL irritation and rupture.

scholarly journals Can Adding BMP2 Improve Outcomes in Patients Undergoing the SUPERhip Procedure?

Children ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 495
Author(s):  
Dror Paley ◽  
Claire E. Shannon ◽  
Monica Nogueira ◽  
Catharina Chiari ◽  
Matthew Harris
Keyword(s):  
Femoral Neck ◽  
Internal Fixation ◽  
Varus Deformity ◽  
Drill Hole ◽  
Late Complications ◽  
Coxa Vara ◽  
Fixed Angle ◽  
Extremity Reconstruction ◽  
Delayed Ossification ◽  
Fixed Angle Device

Congenital femoral deficiency (CFD) Paley type 1b is characterized by severe bony deformity of the upper femur, extra-articular contractures of the hip, and, delayed ossification of the femoral neck and/or subtrochanteric region. The Systematic Utilitarian Procedure for Extremity Reconstruction of the hip (SUPERhip) procedure for the correction of CFD deformities was developed in 1997. Initially, a non-fixed angle device (rush rod) was used for fixation. Late complications of persistent delayed ossification and recurrent varus deformity occurred. In order to reduce and treat such complications, fixation with a fixed angle device and the off-label use of BMP2 to induce ossification of the un-ossified femoral neck were employed. The purpose of this study is to determine if the use of a fixed angle device, and, BMP2 inserted into a drill hole in the cartilage of the femoral neck, decreases the incidence of these late complications. We retrospectively reviewed 72 SUPERhip procedures performed for Paley type 1b CFD between 1997 and 2012. Due to recurrent varus or persistent delayed ossification of the femoral neck, 34 revision SUPERhip procedures were performed. In total, 106 SUPERhip procedures were studied. Sixty-eight SUPERhips were performed using internal fixation without BMP2, while 38 SUPERhips were performed with both internal fixation and the addition of BMP2. Forty-one were performed using non-fixed angle internal fixation while 65 had fixed angle internal fixation. Fixed angle devices significantly reduced the incidence of recurrent varus compared with non-fixed angle devices. Inserting BMP2 in the femoral neck significantly reduced the incidence of persistent delayed ossification. Using only a fixed angle device but no BMP2 did not reduce the incidence of delayed ossification. The combination of both a fixed angle device and BMP2 reduced the incidence of recurrent coxa vara and persistent delayed ossification of the femoral neck. The SUPERhip procedure corrects the pathoanatomy of the proximal femur in CFD Paley type 1b but is associated with a very high risk of recurrence of coxa vara and persistence of femoral neck delayed ossification, unless, a fixed angle internal fixation device is used to prevent recurrent coxa vara and BMP2 is used to induce ossification of the femoral neck.

scholarly journals Tissue-specific organelle DNA degradation mediated by DPD1 exonuclease

2011 ◽  
Vol 6 (9) ◽  
pp. 1391-1393 ◽  
Author(s):  
Lay Yin Tang ◽  
Wataru Sakamoto
Keyword(s):  
Dna Degradation ◽  
Tissue Specific ◽  
Organelle Dna

Immunogold-Silver Staining (IGSS) of (NCP and CP) BVDV Infected Subcellular Fraction Bands

1997 ◽  
Vol 3 (S2) ◽  
pp. 155-156
Author(s):  
C.E. Hearne ◽  
D.L. Johnson ◽  
H. Van Campen
Keyword(s):  
Subcellular Fraction ◽  
Membrane Vesicles ◽  
Bovine Viral Diarrhea ◽  
Fixed Angle ◽  
Cell Monolayers ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Transmission Electron ◽  
Viral Diarrhea Virus ◽  
Bovine Turbinate

Pre-embedding immunogold-silver (IGSS) techniques are useful to localize antigens in cell monolayers and agarose embedded cell suspensions for transmission electron microscopy. Procedural centrifugations, however, present a challenge when attempting to localize antigens in subcellular fractions. Using a Beckman Airfuge Ultracentrifuge to concentrate the subcellar fraction bands and resuspending the organelles in agarose simplifies IGSS processing and resin embedding procedures.Control bovine turbinate (BT), and BT cells infected with cytopathic (cp NADL) and non-cytopathic (ncp NY-1) strains of bovine viral diarrhea virus (BVDV) were fractioned according to Bienz et al (1992). Bands containing membrane vesicles (Fig 2) were collected and each fraction band was pelleted at 169,000g for 20 min using an A-95 fixed angle rotor in a Beckman Airfuge Ultracentrifuge. Each fraction pellet was resuspended in 50μl of 30% agarose, solidified, and trimmed to < lmm. IGSS procedures were carried out according to Nanoprobes, Inc., Stoney Brook, NY, and Hearne & Van Campen (1996).

Treating patella fractures with a fixed-angle patella plate—A prospective observational study

Injury ◽  
2016 ◽  
Vol 47 (8) ◽  
pp. 1737-1743 ◽  
Author(s):  
Michael Wild ◽  
Kai Fischer ◽  
Florian Hilsenbeck ◽  
Mohssen Hakimi ◽  
Marcel Betsch
Keyword(s):  
Observational Study ◽  
Prospective Observational Study ◽  
Fixed Angle ◽  
Patella Fractures

scholarly journals Effect of substrate on the adsorption of polyelectrolyte multilayers: Study by optical fixed-angle reflectometry and AFM

2007 ◽  
Vol 305 (1-3) ◽  
pp. 105-111 ◽  
Author(s):  
C.C. Buron ◽  
C. Filiâtre ◽  
F. Membrey ◽  
C. Bainier ◽  
D. Charraut ◽  
...  
Keyword(s):  
Polyelectrolyte Multilayers ◽  
Fixed Angle

scholarly journals Comparative phylogeography clarifies the complexity and problems of continental distribution that drove A. R. Wallace to favor islands

2016 ◽  
Vol 113 (29) ◽  
pp. 7970-7977 ◽  
Author(s):  
Brett R. Riddle
Keyword(s):  
Nuclear Dna ◽  
Working Relationships ◽  
Distribution Models ◽  
Comparative Phylogeography ◽  
Organelle Dna ◽  
Geographic Structure ◽  
Catching Up ◽  
Geographic Context ◽  
Distributional Dynamics ◽  
Landmark Study

Deciphering the geographic context of diversification and distributional dynamics in continental biotas has long been an interest of biogeographers, ecologists, and evolutionary biologists. Thirty years ago, the approach now known as comparative phylogeography was introduced in a landmark study of a continental biota. Here, I use a set of 455 studies to explore the current scope of continental comparative phylogeography, including geographic, conceptual, temporal, ecological, and genomic attributes. Geographically, studies are more frequent in the northern hemisphere, but the south is catching up. Most studies focus on a Quaternary timeframe, but the Neogene is well represented. As such, explanations for geographic structure and history include geological and climatic events in Earth history, and responses include vicariance, dispersal, and range contraction-expansion into and out of refugia. Focal taxa are biased toward terrestrial or semiterrestrial vertebrates, although plants and invertebrates are well represented in some regions. The use of various kinds of nuclear DNA markers is increasing, as are multiple locus studies, but use of organelle DNA is not decreasing. Species distribution models are not yet widely incorporated into studies. In the future, continental comparative phylogeographers will continue to contribute to erosion of the simple vicariance vs. dispersal paradigm, including exposure of the widespread nature of temporal pseudocongruence and its implications for models of diversification; provide new templates for addressing a variety of ecological and evolutionary traits; and develop closer working relationships with earth scientists and biologists in a variety of disciplines.

Fixed-angle rotary shear as a new method for tailoring electro-mechanical properties of templated graphene–polymer composites

2014 ◽  
Vol 100 ◽  
pp. 70-75 ◽  
Author(s):  
Nicholas Heeder ◽  
Abayomi Yussuf ◽  
Indrani Chakraborty ◽  
Michael P. Godfrin ◽  
Robert Hurt ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Polymer Composites ◽  
New Method ◽  
Fixed Angle ◽  
Rotary Shear
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