The effect of dietary fat on fatty acid synthesis in cell-free preparations of lactating mammary gland

Lipids ◽  
1966 ◽  
Vol 1 (1) ◽  
pp. 76-80 ◽  
Author(s):  
John G. Coniglio ◽  
Raymond Bridges
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Dietary Fat ◽  
Acid Synthesis ◽  
Lactating Mammary Gland

scholarly journals The effect of dietary fat on lipogenesis in mammary gland and liver from lactating and virgin mice

1969 ◽  
Vol 115 (4) ◽  
pp. 807-815 ◽  
Author(s):  
Stuart Smith ◽  
Harriet T. Gagné ◽  
Dorothy R. Pitelka ◽  
S. Abraham
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Dietary Fat ◽  
High Fat Diet ◽  
Corn Oil ◽  
Acid Synthesis ◽  
High Fat ◽  
C3h Mice ◽  
Parenchymal Cells

1. Virgin and lactating C3H mice maintained on laboratory chow were transferred to a high-fat (15% corn oil) or a fat-free diet 3 days before being killed. 2. The linoleate content of liver, mammary gland and milk was decreased in lactating mice given the fat-free diet but was increased in those fed on the high-fat diet. Changes in linoleate content and mammary gland followed a similar but much less marked trend in virgin animals. 3. Hepatic fatty acid synthesis in lactating and virgin mice fed on the fat-free diet was higher than in corresponding animals fed on either the chow or the high-fat diet. The lipogenic capacity of livers from mice fed on either the chow or the high-fat diet was greater in lactating than in virgin animals. These changes in hepatic lipogenic capacity were accompanied by alterations in the specific activities of certain enzymes involved in fat synthesis. 4. Mammary gland from virgin and lactating animals showed no such adaptation to dietary fat. Results indicate that fatty acid synthesis in neither mammary-gland parenchymal cells nor mammary-gland adipose cells can be influenced by dietary fat in the same way as in the hepatocyte.

scholarly journals An intact SREBP1 pathway is essential for the t ‐10, c ‐12 CLA‐induced inhibition of de novo fatty acid synthesis in the murine lactating mammary gland

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Monica R. Foote ◽  
Jenifer Monks ◽  
Kevin J. Harvatine ◽  
Margaret C. Neville ◽  
Yves R. Boisclair ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
De Novo ◽  
Acid Synthesis ◽  
Lactating Mammary Gland

scholarly journals Fatty acid synthase is required for mammary gland development and milk production during lactation

2014 ◽  
Vol 306 (10) ◽  
pp. E1132-E1143 ◽  
Author(s):  
Janel Suburu ◽  
Lihong Shi ◽  
Jiansheng Wu ◽  
Shihua Wang ◽  
Michael Samuel ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Milk Production ◽  
Fatty Acid Synthesis ◽  
Fatty Acid Synthase ◽  
Mammary Gland Development ◽  
De Novo ◽  
Acid Synthesis ◽  
Lactating Mammary Gland ◽  
Gland Development

The mammary gland is one of the few adult tissues that strongly induce de novo fatty acid synthesis upon physiological stimulation, suggesting that fatty acid is important for milk production during lactation. The committed enzyme to perform this function is fatty acid synthase (FASN). To determine whether de novo fatty acid synthesis is obligatory or dietary fat is sufficient for mammary gland development and function during lactation, Fasn was specifically knocked out in mouse mammary epithelial cells. We found that deletion of Fasn hindered the development and induced the premature involution of the lactating mammary gland and significantly decreased medium- and long-chain fatty acids and total fatty acid contents in the milk. Consequently, pups nursing from Fasn knockout mothers experienced growth retardation and preweanling death, which was rescued by cross-fostering pups to a lactating wild-type mother. These results demonstrate that FASN is essential for the development, functional competence, and maintenance of the lactating mammary gland.

Mammary gland enzyme systems in the study of fatty-acid synthesis

1958 ◽  
Vol 149 (936) ◽  
pp. 414-420
Keyword(s):  
Fatty Acids ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Short Chain Fatty Acids ◽  
Acid Synthesis ◽  
Short Chain ◽  
Mammary Tissue ◽  
Lactating Mammary Gland ◽  
Chain Fatty Acids

1. Introduction: The role of acetate in fatty-acid synthesis In 1945 Folley and his colleagues (see Foiley 1949, 1952; Malpress 1946) suggested that, in the ruminant, short-chain fatty acids might be synthesized by the lactating mammary gland from acetate. These short-chain fatty acids might then serve as precursors for the synthesis of long-chain acids. Folley & French (1949, 1950) showed that in vitro slices of lactating ruminant mammary gland were able to synthesize fat from acetate, as indicated by a respiratory quotient greater than unity. Non-ruminant lactating mammary tissue was unable to do so, but could utilize glucose for fat synthesis. It was later shown, with the aid of tracers (Balmain, Folley & Glascock 1954) that non-ruminant lactating mammary gland slices could use acetate for the synthesis of fat, provided that glucose was also added. Experiments in vivo laid emphasis upon the synthesis of short-chain fatty acids by the mammary gland. Popják & Beeckmans (1950) showed that injection of [ carboxy - 14 C]acetate into the pregnant rabbit gave rise to a high degree of labelling in the mammary gland fat. Fractionation of these fatty acids (Popják, Folley & French 1950) showed that the label was predominantly concentrated in the short-chain fatty acids, and that this labelling was far higher than that found in the liver fatty acids, indicating synthesis in the gland itself.

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