Assaying chimeric genes in plants: The GUS gene fusion system

1987 ◽  
Vol 5 (4) ◽  
pp. 387-405 ◽  
Author(s):  
Richard A. Jefferson
1999 ◽  
Vol 67 (12) ◽  
pp. 6695-6697 ◽  
Author(s):  
Stephan Köhler ◽  
Safia Ouahrani-Bettache ◽  
Marion Layssac ◽  
Jacques Teyssier ◽  
Jean-Pierre Liautard

ABSTRACT A gene fusion system based on plasmid pBBR1MCS and the expression of green fluorescent protein was developed for Brucella suis, allowing isolation of constitutive and inducible genes. Bacteria containing promoter fusions of chromosomal DNA togfp were visualized by fluorescence microscopy and examined by flow cytometry. Twelve clones containing gene fragments induced inside J774 murine macrophages were isolated and further characterized.


Gene ◽  
1987 ◽  
Vol 58 (1) ◽  
pp. 87-97 ◽  
Author(s):  
Björn Löwenadler ◽  
Birger Jansson ◽  
Suzanne Paleus ◽  
Erik Houngren ◽  
Björn Nilsson ◽  
...  

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