Mass spectroscopy: A tool now available to the fatty acid chemist

1966 ◽  
Vol 43 (8) ◽  
pp. 338A-340A
Author(s):  
Robert T. O'Connor
Keyword(s):  
Fatty Acid ◽  
Mass Spectroscopy

scholarly journals Steroid and Fatty Acid Contents from the Leaves of Carica Papaya

Folia Medica ◽  
2021 ◽  
Vol 63 (3) ◽  
pp. 422-428
Author(s):  
Vishvraj Devmurari ◽  
Poojaben Patel ◽  
Rajeshreeba Jadeja ◽  
Cameykumari Bhadaniya ◽  
Priti Aghara ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Fatty Acid ◽  
Mass Spectroscopy ◽  
Carica Papaya ◽  
Methyl Esters ◽  
Petroleum Ether Extract ◽  
Current Method ◽  
Direct Analysis ◽  
Polar Compounds ◽  
Saponifiable Matter

Aim: To extract and identify the non-polar entities from the leaves of Carica papaya, a plant used for medicinal purpose as folk medicine.Materials and methods: Petroleum ether extract of the Carica papaya leaves was used for this study. Saponification process and methylation process was performed to separate fatty acids and unsaponifiable matters. Phytochemical constituents were separated using chemical process and separated fractions were analyzed by thin layer chromatography (TLC) and gas chromatography coupled with mass spectroscopy (GC-MS).Results: The chemical composition of the steroids, triterpenoids and fatty acid methyl esters (FAMEs) in leaves of Carica papaya, which were analyzed by gas chromatography coupled with mass spectroscopy (GC-MS). A total of 15 fatty acid components were identified in saponifiable matter, from unsaponifiable portion 2 steroids (campesterol, β- or γ-sitosterol), 1 triterpene (squalene), and 1 diterpene (phytol) were identified.Conclusions: The results indicate that the extract is rich in non-polar compounds. In this study, GC-MS method is at the central focus for identification of these phytoconstituents. The current method can be used for direct analysis of non-polar entities of plant material.

scholarly journals In vivo antimalarial effect of Ananas comosus (L) Merr (Bromeliaceae) fruit peel, and gas chromatography-mass spectroscopy profiling: A possible role for polyunsaturated fatty acid

2020 ◽  
Vol 19 (1) ◽  
pp. 137-145 ◽  
Author(s):  
Philip F. Uzor ◽  
Blessing U Ishiwu ◽  
Ngozi J. Nwodo
Keyword(s):  
Gas Chromatography ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Polyunsaturated Fatty Acid ◽  
Mass Spectroscopy ◽  
Artemisinin Combination Therapy ◽  
Inhibitory Effect ◽  
Ananas Comosus ◽  
Fruit Peel ◽  
Gas Chromatography Mass Spectroscopy

Purpose: To evaluate the antimalarial effect of Ananas comosus extract and fractions and also to identify the likely bioactive compounds.Method: The fruit peel of the plant was extracted with methanol, and the extract successively fractionated with n-hexane, dichloromethane, ethyl acetate, methanol and water. The n-hexane fraction was further subjected to vacuum liquid chromatography to afford four sub-fractions, one of which was also analyzed using gas chromatography-mass spectroscopy (GC-MS). Plasmodium berghei-infected mice were treated orally with three doses (100, 200 and 400 mg/kg) of the plant extract and a single dose (200 mg/kg) of each of the fractions and sub-fractions in a curative antimalarial model using artemisinin combination therapy (ACT) as the reference drug.Results: The extract exhibited significant (p < 0.001) non-dose dependent parasitemia inhibitory activity in the range of 44.84 to 76.09 %. All fractions displayed inhibitory effect (p < 0.001) in the range of 46.44 to 87.58 % with the dichloromethane fraction displaying the highest effect (87.58 %). The subfractions exhibited significant inhibitory effect (p < 0.001) in the range of 84.14 to 92.54 %. The ACT produced significant (p < 0.001) inhibitory effect of 83.92 %. GC-MS analysis revealed the presence of 17 bioactive compounds, the most abundant of which were linoleic acid and palmitic acid.Conclusion: A. comosus displays strong antimalarial activity which supports the folkloric use of the plant for malarial treatment. A polyunsaturated fatty acid (linoleic acid) was the most abundant phytoconstituent identified. Keywords: Ananas comosus, Antimalarial, Malaria, Pineapple, Plasmodium beighei

scholarly journals A new series of long-chain dicarboxylic acids with vicinal dimethyl branching found as major components of the lipids of Butyrivibrio spp

1979 ◽  
Vol 183 (3) ◽  
pp. 691-700 ◽  
Author(s):  
R A Klein ◽  
G P Hazlewood ◽  
P Kemp ◽  
R M C Dawson
Keyword(s):  
Molecular Weight ◽  
Fatty Acid ◽  
High Molecular Weight ◽  
Mass Spectroscopy ◽  
Major Part ◽  
Dicarboxylic Acids ◽  
Synthetic Compounds ◽  
High Molecular Weight Component ◽  
Complex Lipids ◽  
Long Chain

1. Some members of the genus Butyrivibrio, including a general fatty acid auxotroph (strain S2), contain as a major part of their complex lipids a high-molecular-weight component that is probably formed by the union of two fatty acid chains [Hazlewood & Dawson (1979) J. Gen. Microbiol. 112, 15–27]. 2. Proton and 13C n.m.r. and i.r. and mass spectroscopy were used to examine a homologous series of these moieties and, in addition, the hydrocarbon derivative of one homologue and several synthetic compounds. 3. The results indicate that the high-molecular-weight components are a series of long-chain dicarboxylic acids containing vicinal dimethyl branching, located near the centre of the chain.

scholarly journals ANALYSIS OF FREE FATTY ACID ON SOYBEAN OIL USING GAS CHROMATOGRAPHY – MASS SPECTROSCOPY

2010 ◽  
Vol 4 (1) ◽  
pp. 62-67
Author(s):  
Deni Pranowo ◽  
M Muchalal
Keyword(s):  
Gas Chromatography ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Soybean Oil ◽  
Mass Spectroscopy ◽  
Sodium Methoxide ◽  
Transesterification Reaction ◽  
Gas Chromatography Mass Spectroscopy

Research on transesterification of soybean oil with sodium methoxide as the catalyst in methanol has been conducted. Yields of transesterification reaction were analyzed by gas chromatography-mass spectroscopy (GC-MS). GC-MS spectrum showed that mehtyl palmitic, methyl stearic, methyl oleic and methyl linoleic were produced from the reaction. The reaction was done for 90 minutes and gave convertion of 84.53% (b/b).   Keywords: transesterification, soybean oil

Temperature Dependence of the Critical Electron Dose for Fatty Acid Monolayers

1982 ◽  
Vol 40 ◽  
pp. 78-79
Author(s):  
Kenneth H. Downing ◽  
Robert M. Glaeser
Keyword(s):  
Electron Beam ◽  
Fatty Acid ◽  
Inelastic Scattering ◽  
Temperature Dependence ◽  
Structural Damage ◽  
Direct Result ◽  
Second Step ◽  
Strong Temperature Dependence ◽  
Electron Dose ◽  
Covalent Bonds

The structural damage of molecules irradiated by electrons is generally considered to occur in two steps. The direct result of inelastic scattering events is the disruption of covalent bonds. Following changes in bond structure, movement of the constituent atoms produces permanent distortions of the molecules. Since at least the second step should show a strong temperature dependence, it was to be expected that cooling a specimen should extend its lifetime in the electron beam. This result has been found in a large number of experiments, but the degree to which cooling the specimen enhances its resistance to radiation damage has been found to vary widely with specimen types.

Effect of light intensity on the ultrastructure of the filamentous cyanobacterium, Anabaena variabilis

1988 ◽  
Vol 46 ◽  
pp. 300-301
Author(s):  
C. S. Bricker ◽  
S. R. Barnum ◽  
B. Huang ◽  
J. G. Jaworskl
Keyword(s):  
Fatty Acid ◽  
Light Intensity ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Anabaena Variabilis ◽  
Chloroplast Envelope ◽  
Major Constituent ◽  
Filamentous Cyanobacterium ◽  
Photosynthetic Membrane ◽  
Effect Of Light

Cyanobacteria are Gram negative prokaryotes that are capable of oxygenic photosynthesis. Although there are many similarities between eukaryotes and cyanobacteria in electron transfer and phosphorylation during photosynthesis, there are two features of the photosynthetic apparatus in cyanobacteria which distinguishes them from plants. Cyanobacteria contain phycobiliproteins organized in phycobilisomes on the surface of photosynthetic membrane. Another difference is in the organization of the photosynthetic membranes. Instead of stacked thylakolds within a chloroplast envelope membrane, as seen In eukaryotes, IntracytopIasmlc membranes generally are arranged in three to six concentric layers. Environmental factors such as temperature, nutrition and light fluency can significantly affect the physiology and morphology of cells. The effect of light Intensity shifts on the ultrastructure of Internal membrane in Anabaena variabilis grown under controlled environmental conditions was examined. Since a major constituent of cyanobacterial thylakolds are lipids, the fatty acid content also was measured and correlated with uItrastructural changes. The regulation of fatty acid synthesis in cyanobacteria ultimately can be studied if the fatty acid content can be manipulated.

The effects of low-ratio n-6/n-3 PUFA on biomarkers of inflammation: a systematic review and meta-analysis

2021 ◽  
Author(s):  
Yali Wei ◽  
Yan Meng ◽  
Na Li ◽  
Qian Wang ◽  
Liyong Chen
Keyword(s):  
Systematic Review ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Meta Analysis ◽  
Chain Polyunsaturated Fatty Acid ◽  
Inflammation Markers ◽  
Pufa Supplementation ◽  
Long Chain

The purpose of the systematic review and meta-analysis was to determine if low-ratio n-6/n-3 long-chain polyunsaturated fatty acid (PUFA) supplementation affects serum inflammation markers based on current studies.

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