Quantitative determination of traces of free gossypol in fats, oils, and fatty acids by paper chromatography

1958 ◽  
Vol 35 (7) ◽  
pp. 371-373 ◽  
Author(s):  
Guenter Schramm ◽  
James H. Benedict
Keyword(s):  
Fatty Acids ◽  
Quantitative Determination ◽  
Paper Chromatography ◽  
Free Gossypol

Quantitative Untersuchungen über die Ommochromvorstufen in den Malpighischen Gefäßen verschiedener Drosophila-Mutanten

1968 ◽  
Vol 23 (4) ◽  
pp. 547-554 ◽  
Author(s):  
Dieter Eichelberg
Keyword(s):  
Sex Differences ◽  
Drosophila Melanogaster ◽  
Quantitative Determination ◽  
Paper Chromatography ◽  
Type A ◽  
Individual Development ◽  
Wild Type ◽  
Strong Reduction ◽  
The Individual

This paper concerns with the quantitative determination of ommochrome precursors in the Malpighian tubes of Drosophila melanogaster during the individual development. After separation by paper chromatography the amounts of tryptophane, kynurenine and 3-hydroxykynurenine have been estimated by a spectrophotometer. The concentrations of these three substances obtained from wild-type Malpighian tubes have been compared with the quantities of the mutants brown (bw) and red Malpighian tubes (red). During development there are significant variabilities in contents of tryptophane, kynurenine and 3-hydroxykynurenine in the Malpighian tubes. In the larval tubes large quantities of ommochrome precursors are accumulated. With the beginning of metamorphosis there is a distinct decrease in these substances. After hatching the amount increases steadily until reaching a constant level. In the Malpighian tubes there are also sex differences: in females the concentration of kynurenine and 3-hydroxykynurenine is higher than in males. The results obtained from the mutants brown and red Malpighian tubes are on principle the same as those obtained from wild-type. A strong reduction of kynurenine contents is found in the mutant red Malpighian tubes. Perhaps in this mutant the kynurenine-hydroxilase-activity is lower than in wild-type. The amounts of ommochrome precursors, accumulated in the larval Malpighian tubes, do not correspond in all cases to the contents of xanthommatine formed in the eyes of the adults.

A Simplified Halphen Procedure for Cyclopropene Fatty Acids

1972 ◽  
Vol 55 (6) ◽  
pp. 1288-1293
Author(s):  
Eugene C Coleman ◽  
David Firestone
Keyword(s):  
Fatty Acids ◽  
Quantitative Determination ◽  
Closed System ◽  
Fats And Oils ◽  
The Other ◽  
Solvent Systems ◽  
Cyclopropene Fatty Acids ◽  
Reaction Media ◽  
Qualitative Analyses

Abstract A simplified Halphen procedure was developed for the quantitative determination of cyclopropene fatty acids in fats and oils. Butanol, dimethyl sulfoxide (DMSO), and mixtures of butanol and DMSO were used as reaction media in a closed system. Higher sample absorbances were produced in butanol than in mixtures of butanol and DMSO. Butanol was superior to the other solvent systems for both quantitative and qualitative analyses. The lower limit of sensitivity for the tube-butanol system for quantitative and qualitative analyses was 18 and 15 μg cyclopropene fatty acids/g oil, respectively.

Quantitative determination of serum triglycerides by glass-fiber paper chromatography

1964 ◽  
Vol 8 (2) ◽  
pp. 158-163 ◽  
Author(s):  
Karoly G. Pinter ◽  
James G. Hamilton ◽  
O. Neal Miller
Keyword(s):  
Quantitative Determination ◽  
Glass Fiber ◽  
Paper Chromatography ◽  
Serum Triglycerides
Sign in / Sign up

Export Citation Format

Share Document