Neutral sugar analysis of polysaccharides from the seed epidermis ofBrassia campestris

1987 ◽  
Vol 64 (5) ◽  
pp. 761-762 ◽  
Author(s):  
L. Van Caeseele ◽  
M. I. P. Kovacs ◽  
Rosalie Glilespie
Keyword(s):  
Neutral Sugar ◽  
Sugar Analysis ◽  
Seed Epidermis

Strain distribution and in planta production of an extracellular polysaccharide depolymerase from Bradyrhizobium japonicum

1992 ◽  
Vol 38 (8) ◽  
pp. 857-861 ◽  
Author(s):  
Michael F. Dunn ◽  
Arthur L. Karr
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Root Nodules ◽  
Extracellular Polysaccharide ◽  
Extracellular Polysaccharides ◽  
Neutral Sugar ◽  
In Planta ◽  
In Vitro Production ◽  
Polysaccharide Composition ◽  
Vitro Production

Thirty-four strains of Bradyrhizobium japonicum were screened for the in vitro production of an extracellular polysaccharide depolymerase active against the B. japonicum acidic extracellular polysaccharide that contains mannose, glucose, galactose, and 4-O-methylgalactose as neutral sugar components. Over 90% of tested strains producing this type of extracellular polysaccharide also produced the extracellular polysaccharide depolymerase, whereas strains producing a compositionally different extracellular polysaccharide did not. In addition, representatives of species related to B. japonicum by extracellular polysaccharide composition or host range were also phenotypically depolymerase negative. Depolymerase was also present in soybean root nodules formed by B. japonicum strain 2143. In contrast to the cell-associated depolymerase activity found in free-living cells of this strain, most of the depolymerase activity present in nodules is free of the bacteroids. The widespread occurrence of the depolymerase among B. japonicum strains and the spatiotemporal distribution of its activity in planta are consistent with the enzyme playing a role in the removal of surface extracellular polysaccharide from the microorganism during the infection of nodulation process. Key words: Bradyrhizobium japonicum, soybean, extracellular polysaccharides, extracellular polysaccharide depolymerase, bacteroids.

scholarly journals Resolution, purification and some properties of the multiple forms of cellobiose quinone dehydrogenase from the white-rot fungus Sporotrichum pulverulentum

1986 ◽  
Vol 236 (1) ◽  
pp. 221-226 ◽  
Author(s):  
F F Morpeth ◽  
G D Jones
Keyword(s):  
Superoxide Anion ◽  
Sugar Content ◽  
White Rot ◽  
Sedimentation Equilibrium ◽  
White Rot Fungus ◽  
Neutral Sugar ◽  
Apparent Difference ◽  
Multiple Forms ◽  
Sporotrichum Pulverulentum ◽  
Similar Kinetic

Four forms of cellobiose quinone dehydrogenase have been purified from the white-rot fungus Sporotrichum pulverulentum. The Mr of the enzyme has been estimated by sedimentation equilibrium to be 57,800 and by SDS/polyacrylamide-gel to be 60,000. These enzymes are clearly monomers. Cellobiose quinone dehydrogenases contain FAD and variable amounts of a green chromophore which we suggest is 6-hydroxy-FAD. The superoxide anion and H2O2 are the products of its reaction with oxygen. All of the isoenzymes from any one preparation display similar kinetic parameters. However, these vary between preparations. The only apparent difference between the four separable isoenzymes is their neutral-sugar content.

NMR Tube Degradation Method for Sugar Analysis of Glycosides

2016 ◽  
Vol 79 (9) ◽  
pp. 2413-2417 ◽  
Author(s):  
José-Luis Giner ◽  
Ju Feng ◽  
David J. Kiemle
Keyword(s):  
Sugar Analysis

Refining the toolkit for sugar analysis

Nature ◽  
2021 ◽  
Vol 599 (7883) ◽  
pp. 168-169
Author(s):  
Amber Dance
Keyword(s):  
Sugar Analysis

scholarly journals Germination and Early Seedling Development in Quercus ilex Recalcitrant and Non-dormant Seeds: Targeted Transcriptional, Hormonal, and Sugar Analysis

2018 ◽  
Vol 9 ◽  
Author(s):  
M. Cristina Romero-Rodríguez ◽  
Antonio Archidona-Yuste ◽  
Nieves Abril ◽  
Antonio M. Gil-Serrano ◽  
Mónica Meijón ◽  
...  
Keyword(s):  
Quercus Ilex ◽  
Seedling Development ◽  
Sugar Analysis ◽  
Early Seedling ◽  
Early Seedling Development
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