Preparation and properties of methyl arachidonate from pork liver

1959 ◽  
Vol 36 (10) ◽  
pp. 443-449 ◽  
Author(s):  
O. S. Privett ◽  
R. P. Weber ◽  
E. Christense Nickell
2021 ◽  
pp. 26-30
Author(s):  
O.N. Krasulia ◽  
S.A. Myakinina ◽  
E.V. Kazakova ◽  
Yu.A. Shumskiy
Keyword(s):  

2014 ◽  
Vol 20 (11) ◽  
pp. 1945-1947 ◽  
Author(s):  
Christophe Renou ◽  
Anne-Marie Roque-Afonso ◽  
Nicole Pavio

1971 ◽  
Vol 49 (7) ◽  
pp. 829-837 ◽  
Author(s):  
Roger L. Hudgin ◽  
Harry Schachter

The properties of CMP-N acetylneuraminic acid: glycoprotein sialyltransferase have been studied in pork serum, a crude pork liver homogenate, and a soluble acetone powder extract prepared from pork liver. Whereas the crude liver homogenate enzyme is activated by the detergent Triton X-100, this detergent has no effect on the activities of either serum or acetone powder extract; since high-speed centrifugation does not sediment the enzyme activities of the latter two preparations, it is concluded that they are soluble. Comparison of the membrane-bound and soluble liver enzymes indicates that the membrane modifies kinetic behavior only to a limited extent. In both liver and serum, a single sialyltransferase is responsible for incorporation of sialic acid into α1-acid glycoprotein, fetuin, and N-acetyllactosamine, and sialic acid incorporation occurs whenever a terminal galactose linked (β, 1 → 4) to a penultimate N-acetylglucosamine is presented to the enzyme. Although the serum enzyme resembles the liver enzyme, both the source and function of serum sialyltransferase are unknown.


2015 ◽  
Vol 56 (1) ◽  
pp. 41-49
Author(s):  
Jasna Ivanovic ◽  
Snezana Saicic ◽  
Mirjana Milanovic-Stevanovic ◽  
Nada Petrovic ◽  
Irena Zizovic ◽  
...  

Meat Science ◽  
2013 ◽  
Vol 93 (4) ◽  
pp. 880-887 ◽  
Author(s):  
Ana María Martín-Sánchez ◽  
Gelmy Ciro-Gómez ◽  
Estrella Sayas ◽  
José Vilella-Esplá ◽  
Jamel Ben-Abda ◽  
...  

2016 ◽  
Vol 9 (6) ◽  
pp. 970-980 ◽  
Author(s):  
Liselot Steen ◽  
Seline Glorieux ◽  
Olivier Goemaere ◽  
Kristof Brijs ◽  
Hubert Paelinck ◽  
...  

2020 ◽  
Vol 8 (10) ◽  
pp. 1460
Author(s):  
Eva Trojnar ◽  
Matthias Contzen ◽  
Dominik Moor ◽  
Anja Carl ◽  
Sabine Burkhardt ◽  
...  

Background: In the last years, the number of notified hepatitis E cases in humans has continuously increased in Europe. Foodborne infection with the zoonotic hepatitis E virus (HEV) genotype 3 is considered the major cause of this disease. Undercooked liver and raw sausages containing the liver of pigs and wild boar are at high risk of containing HEV. However, so far, no standardized method for the detection of HEV-RNA in pig liver is available. Methods: An international collaborative study on method reproducibility involving 11 laboratories was performed for an HEV-RNA detection method, which consists of steps of sample homogenization, RNA extraction and real-time RT-PCR detection, including a process control. Naturally contaminated pork liver samples containing two different amounts of HEV and a HEV-negative pork liver sample were tested by all laboratories using the method. Results: Valid results were retrieved from 10 laboratories. A specificity of 100% and a sensitivity of 79% were calculated for the method. False negative results were only retrieved from the sample containing very low HEV amounts near the detection limit. Conclusions: The results show that the method is highly specific, sufficiently sensitive and robust for use in different laboratories. The method can, therefore, be applied to routine food control as well as in monitoring studies.


Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4462
Author(s):  
Xing Shen ◽  
Jiahong Chen ◽  
Shuwei Lv ◽  
Xiulan Sun ◽  
Boris B. Dzantiev ◽  
...  

Enrofloxacin (ENR) is a widely used fluoroquinolone (FQ) antibiotic for antibacterial treatment of edible animal. In this study, a rapid and highly specific fluorescence polarization immunoassay (FPIA) was developed for monitoring ENR residues in animal foods. First, ENR was covalently coupled to bovine serum albumin (BSA) to produce specific polyclonal antibodies (pAbs). Three fluorescein-labeled ENR tracers (A, B, and C) with different spacers were synthesized and compared to obtain higher sensitivity. Tracer C with the longest arm showed the best sensitivity among the three tracers. The developed FPIA method showed an IC50 (50% inhibitory concentration) of 21.49 ng·mL−1 with a dynamic working range (IC20–IC80) of 4.30–107.46 ng·mL−1 and a limit of detection (LOD, IC10) of 1.68 ng·mL−1. The cross-reactivity (CR) of several structurally related compounds was less than 2%. The recoveries of spiked pork liver and chicken samples varied from 91.3% to 112.9%, and the average coefficients of variation were less than 3.83% and 5.13%, respectively. The immunoassay took only 8 min excluding sample pretreatment. This indicated that the established method had high sensitivity, specificity, and the advantages of simplicity. Therefore, the proposed FPIA provided a useful screening method for the rapid detection of ENR residues in pork liver and chicken.


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