In vivo compartmental metabolism of13C docosahexaenoic acid, studied by gas chromatography-combustion isotope ratio mass spectrometry

Lipids ◽  
1996 ◽  
Vol 31 (1) ◽  
pp. S109-S115 ◽  
Author(s):  
Martine Croset ◽  
Nicole Brossard ◽  
Christiane Pachiaudi ◽  
Sylvie Normand ◽  
Jean Lecerf ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Docosahexaenoic Acid ◽  
Isotope Ratio ◽  
Isotope Ratio Mass Spectrometry

scholarly journals Docosahexaenoic Acid (DHA) Bioavailability in Humans after Oral Intake of DHA-Containing Triacylglycerol or the Structured Phospholipid AceDoPC®

Nutrients ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 251 ◽  
Author(s):  
Mayssa Hachem ◽  
Houda Nacir ◽  
Madeleine Picq ◽  
Mounir Belkouch ◽  
Nathalie Bernoud-Hubac ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Docosahexaenoic Acid ◽  
Isotope Ratio ◽  
Oral Intake ◽  
Isotope Ratio Mass Spectrometry ◽  
Red Cell ◽  
Acetyl Group ◽  
Dha Enrichment ◽  
The Brain

AceDoPC® is a structured glycerophospholipid that targets the brain with docosahexaenoic acid (DHA) and is neuroprotective in the experimental ischemic stroke. AceDoPC® is a stabilized form of the physiological 2-DHA-LysoPC with an acetyl group at the sn1 position; preventing the migration of DHA from the sn2 to sn1 position. In this study we aimed to know the bioavailability of 13C-labeled DHA after oral intake of a single dose of 13C-AceDoPC®, in comparison with 13C-DHA in triglycerides (TAG), using gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) to assess the 13C enrichment of DHA-containing lipids. 13C-DHA enrichment in plasma phospholipids was significantly higher after intake of AceDoPC® compared with TAG-DHA, peaking after 24 h in both cases. In red cells, 13C-DHA enrichment in choline phospholipids was comparable from both sources of DHA, with a maximum after 72 h, whereas the 13C-DHA enrichment in ethanolamine phospholipids was higher from AceDoPC® compared to TAG-DHA, and continued to increase after 144 h. Overall, our study indicates that DHA from AceDoPC® is more efficient than from TAG-DHA for a sustained accumulation in red cell ethanolamine phospholipids, which has been associated with increased brain accretion.

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