Isolation and characterization of a novel human prostatic stromal cell culture: DuK50

1995 ◽  
Vol 31 (11) ◽  
pp. 840-845 ◽  
Author(s):  
Kathryn M. Roberson ◽  
Darryl W. Edwards ◽  
Gary C. Chang ◽  
Cary N. Robertson
Keyword(s):  
Cell Culture ◽  
Stromal Cell ◽  
Isolation And Characterization ◽  
Stromal Cell Culture

Stimulatory effect of ipriflavone on formation of bone-like tissue in rat bone marrow stromal cell culture

1992 ◽  
Vol 51 (S1) ◽  
pp. S16-S20 ◽  
Author(s):  
Kohei Notoya ◽  
Ryoichi Tsukuda ◽  
Keiji Yoshida ◽  
Shigehisa Taketomi
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Marrow Stromal Cell ◽  
Stromal Cell Culture ◽  
Rat Bone

scholarly journals Isolation and characterization of canine parvovirus type 2c circulating in Uruguay

2011 ◽  
Vol 41 (8) ◽  
pp. 1436-1440 ◽  
Author(s):  
Andrea Blanc Pintos ◽  
Cecilia Beatriz Negro Larrama ◽  
Eduardo Enrique Reolon Baratta ◽  
Mabel Beatriz Berois Barthe ◽  
Juan Ramón Arbiza Rodonz
Keyword(s):  
Cell Culture ◽  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Gene Encoding ◽  
Partial Sequencing ◽  
Phylogenetic Characterization ◽  
Isolation And Characterization ◽  
Restriction Fragment Length

This research reports the first CPV-2c isolation in cell culture (canine fibroma cell line A-72) in Uruguay. The isolates were obtained from 13 rectal swabs of Uruguayan dogs with parvovirosis. Samples were submitted to PCR with two sets of primers, restriction fragment length polymorphism (RFLP), partial sequencing of the gene encoding for VP2 capsid protein and phylogenetic characterization. The strain isolated was confirmed as CPV-2c. These results contribute to a better knowledge of CPV strains circulating in Uruguay and promote an evaluation of the efficacy of heterologous vaccines used to protect against the circulating strains.

scholarly journals Customized hydrogel substrates for serum-free expansion of functional hMSCs

2020 ◽  
Vol 8 (14) ◽  
pp. 3819-3829
Author(s):  
Ngoc Nhi T. Le ◽  
Tianran Leona Liu ◽  
James Johnston ◽  
John D. Krutty ◽  
Kayla Marie Templeton ◽  
...  
Keyword(s):  
Cell Culture ◽  
Design Of Experiments ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Free Expansion ◽  
Serum Free ◽  
Synthetic Hydrogel ◽  
Stromal Cell Culture

Synthetic hydrogel arrays combined with a design of experiments approach identified hydrogel compositions for media-agnostic human mesenchymal stromal cell culture.

scholarly journals Isolation and characterization of hantavirus carried by Apodemus peninsulae in Jilin, China

2007 ◽  
Vol 88 (4) ◽  
pp. 1295-1301 ◽  
Author(s):  
Yong-Zhen Zhang ◽  
Yang Zou ◽  
Lai-Shun Yao ◽  
Guang-Wei Hu ◽  
Zhan-Shen Du ◽  
...  
Keyword(s):  
Cell Culture ◽  
Phylogenetic Analysis ◽  
Eastern China ◽  
Immunofluorescence Assay ◽  
Haemorrhagic Fever ◽  
Apodemus Peninsulae ◽  
Isolation And Characterization ◽  
North Eastern ◽  
Field Mice

To provide a better understanding of hantavirus epidemiology in China, Korean field mice (Apodemus peninsulae) and striped field mice (Apodemus agrarius) were captured in Jilin province, China, where haemorrhagic fever with renal syndrome (HFRS) is endemic. Hantavirus antigens were detected in eight of the 130 A. peninsulae individuals and in four of the 193 A. agrarius individuals by using an immunofluorescence assay. Partial S and M segments were amplified from all of the antigen-positive samples. Furthermore, two hantaviruses (CJAp89 and CJAp93) were isolated successfully in cell culture and the entire S and M segments were amplified from one of them (CJAp93). Phylogenetic analysis of these sequences (partial or complete) showed that hantaviruses carried by A. peninsulae and A. agrarius form two distinct lineages, although viruses carried by A. peninsulae are similar to those isolated previously from A. agrarius in China and from HFRS patients in Russia. However, the viruses detected in A. peninsulae in China are genetically different from those detected in A. peninsulae in other countries. These data suggest that A. peninsulae is also a natural host for HTNV in north-eastern China.

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