Crude liver membrane fractions as substrate preserve liver-specific functions in long-term, serum-free rat hepatocyte cultures

1993 ◽  
Vol 29 (1) ◽  
pp. 32-40 ◽  
Author(s):  
Bashar Saad ◽  
Hanspeter Schawalder ◽  
Peter Maier
Keyword(s):  
Rat Hepatocyte ◽  
Serum Free ◽  
Hepatocyte Cultures ◽  
Liver Membrane

Toxicological properties of thymoquinone in primary rat hepatocyte cultures

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
M Khader ◽  
N Bresgen ◽  
P Eckl
Keyword(s):  
Rat Hepatocyte ◽  
Hepatocyte Cultures

scholarly journals THE EXTRACELLULAR MATRIX CONTROL OF ACUTE PHASE RESPONSE IN RAT HEPATOCYTE CULTURES

Shock ◽  
1995 ◽  
Vol 4 (Supplement) ◽  
pp. 52
Author(s):  
E. Adoeva ◽  
T. Stepanova ◽  
I. Diakonov ◽  
A. Polevschlkov
Keyword(s):  
Extracellular Matrix ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Phase Response ◽  
Rat Hepatocyte ◽  
Hepatocyte Cultures ◽  
Matrix Control

scholarly journals Aurintricarboxylic acid rescues PC12 cells and sympathetic neurons from cell death caused by nerve growth factor deprivation: correlation with suppression of endonuclease activity.

1991 ◽  
Vol 115 (2) ◽  
pp. 461-471 ◽  
Author(s):  
A Batistatou ◽  
L A Greene
Keyword(s):  
Cell Death ◽  
Nerve Growth Factor ◽  
Pc12 Cells ◽  
Sympathetic Neurons ◽  
Neuronal Cell Death ◽  
Neuronal Cell ◽  
Endonuclease Activity ◽  
Term Survival ◽  
Serum Free

Past studies have shown that serum-free cultures of PC12 cells are a useful model system for studying the neuronal cell death which occurs after neurotrophic factor deprivation. In this experimental paradigm, nerve growth factor (NGF) rescues the cells from death. It is reported here that serum-deprived PC12 cells manifest an endonuclease activity that leads to internucleosomal cleavage of their cellular DNA. This activity is detected within 3 h of serum withdrawal and several hours before any morphological sign of cell degeneration or death. NGF and serum, which promote survival of the cells, inhibit the DNA fragmentation. Aurintricarboxylic acid (ATA), a general inhibitor of nucleases in vitro, suppresses the endonuclease activity and promotes long-term survival of PC12 cells in serum-free cultures. This effect appears to be independent of macromolecular synthesis. In addition, ATA promotes long-term survival of cultured sympathetic neurons after NGF withdrawal. ATA neither promotes nor maintains neurite outgrowth. It is hypothesized that the activation of an endogenous endonuclease could be responsible for neuronal cell death after neurotrophic factor deprivation and that growth factors could promote survival by leading to inhibition of constitutively present endonucleases.

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