Development of a serum-free system to study the effect of growth hormone and insulinlike growth factor-I on cultured postembryonic growth plate chondrocytes

1992 ◽  
Vol 28 (4) ◽  
pp. 235-244 ◽  
Author(s):  
Gastón Rosselot ◽  
Anthony M. Reginato ◽  
Roland M. Leach
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Growth Plate ◽  
Growth Plate Chondrocytes ◽  
Free System ◽  
Serum Free ◽  
Factor I ◽  
Insulinlike Growth Factor ◽  
Growth Factor I ◽  
Insulinlike Growth Factor I

Dental Development in Children with Growth Hormone Insensitivity Syndrome: Demirjian Analysis of Serial Panoramic Radiographs

2009 ◽  
Vol 46 (4) ◽  
pp. 409-414 ◽  
Author(s):  
Richard Campbell ◽  
Randy Weinshel ◽  
Philippe Backeljauw ◽  
Stephen Wilson ◽  
Judy Bean ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Tooth Development ◽  
Dental Development ◽  
Panoramic Radiographs ◽  
Factor I ◽  
Insulinlike Growth Factor ◽  
Growth Hormone Insensitivity ◽  
Growth Factor I ◽  
Insulinlike Growth Factor I

Objective: This study evaluates the effects of 8 years of insulinlike growth factor-I therapy on tooth development in patients with growth hormone insensitivity syndrome. Methods: Forty-nine panoramic radiographs were evaluated from eight patients (six boys, two girls). Seven teeth in the mandibular left region were graded according to the Demirjian system. Radiographs were taken at the start of insulinlike growth factor-I therapy and were continued at approximately yearly intervals for 8 years. Results: Three of six boys and one of two girls who began treatment with insulinlike growth factor-I at earlier ages experienced an increase in the rate of tooth development. One of six boys who began treatment with insulinlike growth factor-I at a later age had a slower rate of dental development. The patients had more rapid tooth maturation during the beginning of treatment. By the end of treatment, all patients had normal dental maturity for their age. Conclusions: Treatment of growth hormone insensitivity syndrome with insulinlike growth factor-I appears to lead to an increase in dental maturation, particularly in younger patients. After 8 years all patients had achieved normal dental development.

Serum Ghrelin Concentrations are Increased in Children With Growth Hormone Insensitivity and Decrease During Long-Term Insulinlike Growth Factor-I Treatment

2008 ◽  
Vol 56 (1) ◽  
pp. 26-31 ◽  
Author(s):  
Aysin Uckun-Kitapci ◽  
Andrea M. Haqq ◽  
Jonathan Q. Purnell ◽  
Kenneth Newcomb ◽  
Hakan Gulkesen ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Normal Control ◽  
Blood Draw ◽  
Gh Secretion ◽  
Factor I ◽  
Insulinlike Growth Factor ◽  
Igf I ◽  
Growth Factor I ◽  
Insulinlike Growth Factor I

BackgroundGhrelin increases food intake, body weight, and growth hormone (GH) secretion. Serum concentrations of ghrelin are low in obese hyperinsulinemic persons, are reduced by infusion of insulin into normal-weight subjects, and are increased in underweight hypoinsulinemic patients with anorexia nervosa. Laron syndrome is an autosomal recessive disorder of GH insensitivity that results in decreased insulinlike growth factor-I (IGF-I) synthesis and growth failure. These patients have elevated GH levels, excess adipose tissue, and are insulin resistant. Because IGF-I has insulinlike actions and patients with GH insensitivity syndrome (GHIS) exhibit excess adiposity, we sought to determine whether ghrelin levels were elevated in these patients and potentially regulated by IGF-I replacement.MethodsThirteen children with GHIS and 20 normal control children matched for age, sex, and body mass index underwent complete physical examination and a fasting blood draw at baseline. The GHIS subjects then underwent follow-up fasting blood draws during therapy with human recombinant IGF-I (80-120 μg/kg, given subcutaneously twice daily). Fasting glucose, insulin, and IGF-I concentrations were measured at the time of collection. Fasting total ghrelin levels were measured on stored serum samples.ResultsThe GHIS subjects had 2-fold higher fasting ghrelin levels (2926 ± 1869 pg/mL) compared with the normal control children (1492 ± 493 pg/mL; P = 0.009), and mean ghrelin values were reduced 56% during 6.4 ± 0.2 years of IGF-I replacement (P < 0.05).ConclusionsGrowth hormone resistance and low IGF-I levels are associated with elevated ghrelin levels, which may potentiate GH secretion and adiposity in these children. Suppression of ghrelin during IGF-I treatment suggests a novel mechanism potentially regulating ghrelin levels.

Effects of elevated serum insulinlike growth factor-II on growth hormone and insulinlike growth factor-I mRNA and secretion

Metabolism ◽  
1989 ◽  
Vol 38 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Derrell M. Wilson ◽  
Susan N. Perkins ◽  
James A. Thomas ◽  
Steven Seelig ◽  
Susan A. Berry ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Elevated Serum ◽  
Factor I ◽  
Insulinlike Growth Factor ◽  
Factor Ii ◽  
Growth Factor I ◽  
Insulinlike Growth Factor I
Sign in / Sign up

Export Citation Format

Share Document