Cell culture contamination by mycobacteria

1995 ◽  
Vol 31 (10) ◽  
pp. 735-737 ◽  
Author(s):  
Gertrude Case Buehring ◽  
Miriam Valesco ◽  
Chao-Yang Pan
Keyword(s):  
Cell Culture ◽  
Cell Culture Contamination

A solution to the generalized birthday problem with application to allozyme screening for cell culture contamination

1979 ◽  
Vol 16 (02) ◽  
pp. 242-251 ◽  
Author(s):  
M. H. Gail ◽  
G. H. Weiss ◽  
N. Mantel ◽  
S. J. O'Brien
Keyword(s):  
Cell Culture ◽  
Approximate Solution ◽  
Single Cell ◽  
Cell Line ◽  
General Problem ◽  
Birthday Problem ◽  
Classic Problem ◽  
Recursive Solution ◽  
Cell Culture Contamination

This paper gives the probability that no common allozyme signature is found among n randomly selected cell culture lines when the s mutually exclusive and exhaustive signatures have arbitrary known probabilities. This result is useful in detecting cell culture contamination by a single cell line. This problem is a generalization of the classic problem of finding the probability of no common birthday among n individuals when it is assumed that each individual has a chance 1/s = 1/365 of a birthday on a given day. Both an exact recursive solution and an approximate solution are given for the general problem.

A solution to the generalized birthday problem with application to allozyme screening for cell culture contamination

1979 ◽  
Vol 16 (2) ◽  
pp. 242-251 ◽  
Author(s):  
M. H. Gail ◽  
G. H. Weiss ◽  
N. Mantel ◽  
S. J. O'Brien
Keyword(s):  
Cell Culture ◽  
Approximate Solution ◽  
Single Cell ◽  
Cell Line ◽  
General Problem ◽  
Birthday Problem ◽  
Classic Problem ◽  
Recursive Solution ◽  
Cell Culture Contamination

This paper gives the probability that no common allozyme signature is found among n randomly selected cell culture lines when the s mutually exclusive and exhaustive signatures have arbitrary known probabilities. This result is useful in detecting cell culture contamination by a single cell line. This problem is a generalization of the classic problem of finding the probability of no common birthday among n individuals when it is assumed that each individual has a chance 1/s = 1/365 of a birthday on a given day. Both an exact recursive solution and an approximate solution are given for the general problem.

Henrietta Lacks, HeLa Cells, and Cell Culture Contamination

2009 ◽  
Vol 133 (9) ◽  
pp. 1463-1467 ◽  
Author(s):  
Brendan P. Lucey ◽  
Walter A. Nelson-Rees ◽  
Grover M. Hutchins
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cell Line ◽  
Hela Cells ◽  
Human Cell Line ◽  
Hela Cell Line ◽  
Cell Line Hela ◽  
The World ◽  
Cell Culture Contamination ◽  
Line Hela

Abstract Henrietta Lacks died in 1951 of an aggressive adenocarcinoma of the cervix. A tissue biopsy obtained for diagnostic evaluation yielded additional tissue for Dr George O. Gey's tissue culture laboratory at Johns Hopkins (Baltimore, Maryland). The cancer cells, now called HeLa cells, grew rapidly in cell culture and became the first human cell line. HeLa cells were used by researchers around the world. However, 20 years after Henrietta Lacks' death, mounting evidence suggested that HeLa cells contaminated and overgrew other cell lines. Cultures, supposedly of tissues such as breast cancer or mouse, proved to be HeLa cells. We describe the history behind the development of HeLa cells, including the first published description of Ms Lacks' autopsy, and the cell culture contamination that resulted. The debate over cell culture contamination began in the 1970s and was not harmonious. Ultimately, the problem was not resolved and it continues today. Finally, we discuss the philosophical implications of the immortal HeLa cell line.

Cell Culture Contamination

2017 ◽  
pp. 1-7
Author(s):  
Susanne M Gollin ◽  
Dale W Lewis
Keyword(s):  
Cell Culture ◽  
Cell Culture Contamination
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