Complement-dependent induction of DNA synthesis and cell proliferation in human liver connective tissue cells in vitro

1995 ◽  
Vol 31 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Alvaro N. A. Monteiro ◽  
Radovan Borojevic
Keyword(s):  
Cell Proliferation ◽  
Connective Tissue ◽  
Dna Synthesis ◽  
Human Liver ◽  
Tissue Cells ◽  
Liver Connective Tissue Cells

scholarly journals CONTRIBUTIONS TO THE STUDY OF THE MECHANISM OF THE GROWTH OF CONNECTIVE TISSUE

1913 ◽  
Vol 18 (3) ◽  
pp. 287-298 ◽  
Author(s):  
Alexis Carrel
Keyword(s):  
Connective Tissue ◽  
Culture Medium ◽  
Dynamic Condition ◽  
Real Life ◽  
Rate Of Growth ◽  
Independent Life ◽  
Tissue Cells ◽  
Chick Embryo Heart ◽  
Embryo Heart

When connective tissue cells have been cultivated for a certain length of time in a medium which has been repeatedly changed, a definite relation arises between the rate of growth of the cells and the composition of the medium. It is possible, by adding to the culture medium a given quantity of certain substances, such as embryonic juices, to foresee the extent to which a fragment of tissue composed of a given strain of cells will increase in a given time. The rate of growth of a strain of cells can be accelerated or retarded by the addition to the medium of activating or retarding substances. The dynamic condition of a strain of connective tissue cells, which have been living in a given medium for some time, is not a definitely acquired characteristic, but a temporary state, and is the product or function of the medium in which the cells are living, and is readily modified merely by altering the composition of the medium. A knowledge of the characteristics of the growth of connective tissue described has led to a new result,—the indefinite proliferation of a strain of connective tissue cells outside of the organism. The strain of connective tissue originally obtained from a fragment of chick embryo heart, which had been pulsating in vitro for 104 days, was still actively alive after sixteen months of independent life and more than 190 passages. The rate of proliferation of the connective tissue sixteen months old equalled and even exceeded that of fresh connective tissue taken from an eight day old embryo. It appears, therefore, that time has no effect on the tissues isolated from the organism and preserved by means of the technique described above. During the sixteenth month of life in vitro the cells increased rapidly in number and were able in a short time to produce a large quantity of new tissue. This fact, therefore, definitely demonstrates that the tissues were not in a state of survival, as was the case in certain earlier experiments, but in a condition of real life, since the cells of which they were composed, like microorganisms, multiplied indefinitely in the culture medium.

scholarly journals Insulin Stimulates Goose Liver Cell Growth by Activating PI3K-AKT-mTOR Signal Pathway

2016 ◽  
Vol 38 (2) ◽  
pp. 558-570 ◽  
Author(s):  
Chunchun Han ◽  
Shouhai Wei ◽  
Qi Song ◽  
Fang He ◽  
Xiangping Xiong ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Dna Synthesis ◽  
Protein Content ◽  
Mrna Level ◽  
Signal Pathway ◽  
Mtor Pathway ◽  
Brdu Incorporation ◽  
Primary Hepatocytes

Background/Aims: Recent studies have suggested a crucial role for PI3K-Akt-mTOR pathway in regulating cell proliferation, so we hypothesize that insulin acts goose hepatocellular growth by PI3K-Akt-mTOR signal pathway. Because the physiological status of liver cells in vitro is different from that in vivo, a simplified cell model in vitro was established. Methods: Goose primary hepatocytes were isolated and incubated in either no addition as a control or insulin or PI3K-Akt-mTOR pathway inhibitors or co-treatment with glucose and PI3K-Akt-mTOR pathway inhibitors; Then, cell DNA synthesis and cell cycle analysis were detected by BrdU-incorporation Assay and Flow cytometric analysis; the mRNA expression and protein expression of factors involved in the cell cycle were determined by Real-Time RT-PCR, ELISA, and western blot. Results: Here we first showed that insulin evidently increased the cell DNA synthesis, the mRNA level and protein content of factors involved in the cell proliferation of goose primary hepatocytes. Meanwhile, insulin evidently increased the mRNA level and protein content of factors involved in PI3K-Akt-mTOR pathway. However, the up-regulation of insulin on cell proliferation was decreased significantly by the inhibitors of PBK-Akt-mTOR pathway, LY294002, rapamycin or NVP-BEZ235. Conclusion: These findings suggest that PI3K-Akt-mTOR pathway plays an essential role in insulin-regulated cell proliferation of goose hepatocyte.

Inhibition of pig granulosa cell adhesion and growth in vitro by immunoneutralization of epithelial cadherin

Reproduction ◽  
2000 ◽  
pp. 275-281 ◽  
Author(s):  
KM Kirkup ◽  
AM Mallin ◽  
CA Bagnell
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Dna Synthesis ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Cell Contact ◽  
Mouse Ascites ◽  
Proliferation In Vitro ◽  
E Cadherin

Epithelial cadherin (E-cadherin) is a member of the cadherin family of calcium-dependent cell adhesion molecules and is present in the ovary. Although expression of E-cadherin is high in healthy pig granulosa cells and low in granulosa cells of atretic follicles, the importance of E-cadherin-mediated adhesion in granulosa cell function is unclear. The aim of the present study was to determine the impact of immunoneutralization of E-cadherin on granulosa cell adhesion, DNA synthesis and cell proliferation in vitro. Before attachment, pig granulosa cells were exposed to a monoclonal E-cadherin antibody (DECMA-1) which blocks E-cadherin function. Controls included substitution of the antibody with either mouse ascites fluid or another E-cadherin antibody directed against the cytoplasmic domain and which was therefore inaccessible in intact cells. Both granulosa cell proliferation and insulin-like growth factor I-induced DNA synthesis were inhibited significantly in the presence of DECMA-1 compared with controls (P < 0.05). Control granulosa cells in culture formed large clusters with many cells packed tightly together. However, after 48 h exposure to the function-perturbing E-cadherin antibody, there was a significant decrease in the size of the granulosa cell clusters (P < 0.05) and the degree of cell-cell contact was reduced compared with control cultures. No effects on DNA synthesis, cell proliferation or cell adhesion were observed when DECMA-1 was substituted with either mouse ascites fluid or the antibody specific for the cytoplasmic domain of E-cadherin. In conclusion, these data provide evidence to support the hypothesis that E-cadherin is important for maintaining granulosa cell contact, DNA synthesis and cell proliferation in vitro. These results indicate that E-cadherin plays a fundamental role in maintaining both the structure and function of ovarian follicles.

Aluminum Ions Induce DNA Synthesis But Not Cell Proliferation in Human Fibroblasts In Vitro

2002 ◽  
Vol 86 (1) ◽  
pp. 01-10 ◽  
Author(s):  
Carmen Domínguez ◽  
Antonio Moreno ◽  
Marc Llovera
Keyword(s):  
Cell Proliferation ◽  
Dna Synthesis ◽  
Human Fibroblasts ◽  
Aluminum Ions
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