Somatic embryo maturation from long-term suspension cultures of white spruce (Picea glauca)

1993 ◽  
Vol 29 (3) ◽  
pp. 109-112 ◽  
Author(s):  
David I. Dunstan ◽  
Terry D. Bethune ◽  
Cheryl A. Bock
Keyword(s):  
Somatic Embryo ◽  
Picea Glauca ◽  
White Spruce ◽  
Suspension Cultures ◽  
Embryo Maturation ◽  
Somatic Embryo Maturation

Somatic embryo maturation, germination, and soil establishment of plants of black and white spruce (Picea mariana and Picea glauca)

1990 ◽  
Vol 68 (12) ◽  
pp. 2583-2589 ◽  
Author(s):  
S. M. Attree ◽  
T. E. Tautorus ◽  
D. I. Dunstan ◽  
L. C. Fowke
Keyword(s):  
Abscisic Acid ◽  
Somatic Embryo ◽  
Picea Mariana ◽  
Picea Glauca ◽  
Black Spruce ◽  
White Spruce ◽  
Embryo Maturation ◽  
Black And White ◽  
Somatic Embryo Maturation

Somatic embryo maturation, germination, and soil establishment frequencies were compared for two conifer species, white and black spruce (Picea glauca and Picea mariana). The comparison of the two species regenerated and established in soil under the same conditions showed black spruce to be the most responsive. Shorter exposure times to 32 μM abscisic acid were not as effective as maturation on a medium containing 16 μM abscisic acid for 28 days. This gave similar maturation frequencies for the two species (6–8%), and germination frequencies of 64% for white spruce and over 73% for black spruce. Over 1800 black and white spruce plantlets were recovered, and more than 400 were transferred from in vitro to nonsterile conditions. Sixty percent (160) of the black spruce plantlets survived transfer and continued to grow vigorously. By comparison only 18% (29) of the white spruce plantlets survived, and half of these rapidly produced dormant buds and underwent no further shoot growth. White spruce plants that did not produce dormant buds grew vigorously. These results indicate that there are large differences in the ability of these closely related species to respond to plantlet establishment following regeneration from somatic embryos, and that black spruce is highly responsive to micropropagation by this method. Key words: Picea glauca, Picea mariana, somatic embryogenesis, maturation, germination, soil establishment.

scholarly journals Application of Nursery Testing in Long-Term White Spruce Improvement Programs

2007 ◽  
Vol 24 (4) ◽  
pp. 296-300 ◽  
Author(s):  
Yuhui Weng ◽  
Kathy Tosh ◽  
Yill Sung Park ◽  
Michele S. Fullarton
Keyword(s):  
Picea Glauca ◽  
Genetic Correlations ◽  
Field Tests ◽  
Field Performance ◽  
White Spruce ◽  
Height Growth ◽  
Positive Assortative Mating ◽  
The Family ◽  
Improvement Programs

Abstract Polycross-pollinated white spruce (Picea glauca [Moench] Voss) families were evaluated in field and retrospective nursery tests in 1989, 1991, and 1992, respectively. Height growth was measured at age 10 for the field tests and at ages 1 to 6 for the retrospective nursery tests. Except for a few cases, the family mean correlations between nursery and field heights were significant for the 1989 and 1992 series, and their corresponding genetic correlations ranged from low to medium (from 0.37 to 0.74). Because of heavy noncrop competition, height growth in the 1991 nursery series showed consistently lower heritabilities and correlations with field performance compared with those of the other two series. Early nursery selection by theoretical prediction was generally efficient for the 1989 and 1992 series. Rank classification analysis indicated that application of early nursery selection should be used with caution for identifying elite families but could be used to cull inferior families or clones, apply multiple-stage selection, or perform positive assortative mating.

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