Effect of mannitol and glucose-induced osmotic stress on growth, water relations, and solute composition of cell suspension cultures of poplar (Populus deltoides var.Occidentalis) in relation to anthocyanin accumulation

1994 ◽  
Vol 30 (3) ◽  
pp. 164-170 ◽  
Author(s):  
Ashok Tholakalabavi ◽  
Janusz J. Zwiazek ◽  
Trevor A. Thorpe
Keyword(s):  
Osmotic Stress ◽  
Cell Suspension ◽  
Water Relations ◽  
Populus Deltoides ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Anthocyanin Accumulation ◽  
Solute Composition

scholarly journals Establishment and Characterization of Callus and Cell Suspension Cultures of Selected Sorghum bicolor (L.) Moench Varieties: A Resource for Gene Discovery in Plant Stress Biology

Agronomy ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 218 ◽  
Author(s):  
Elelwani Ramulifho ◽  
Tatenda Goche ◽  
Johann Van As ◽  
Toi John Tsilo ◽  
Stephen Chivasa ◽  
...  
Keyword(s):  
Cell Culture ◽  
Osmotic Stress ◽  
Cell Suspension ◽  
Callus Induction ◽  
Callus Formation ◽  
Plant Stress ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Friable Callus ◽  
Stress Biology

Sorghum, a naturally drought tolerant crop, is genetically diverse and provides a wide gene pool for exploitation in crop breeding. In this study, we experimentally assessed friable callus induction rates of seven sorghum varieties using shoot explant for the generation of cell suspension cultures. The cell suspensions were characterized in terms of cell growth and viability profiles as well as gene expression following 400 mM sorbitol-induced osmotic stress for 72 h. Only ICSB 338, a drought susceptible variety, was readily amenable to friable callus formation. Cell culture growth plots of both ICSB 338 and White sorghum (used as a reference line) depicted typical sigmoidal curves. Interestingly, Evans blue assay showed that ICSB 338 cell cultures are more susceptible to osmotic stress than the White sorghum cells. The osmotic stress treatment also triggered differential expression of eight target genes between the two cell culture lines. Overall, these results suggest that the genetic diversity of sorghum germplasm influences friable callus induction rates and molecular responses to osmotic stress, and could be further exploited in plant stress biology studies. Therefore, we have developed a valuable resource for use in molecular studies of sorghum in response to a range of biotic and abiotic stresses.

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