Comparison of cell wall regeneration on maize protoplasts isolated from leaf tissue and suspension cultured cells

1991 ◽  
Vol 27 (2) ◽  
pp. 70-76 ◽  
Author(s):  
Hong Wang ◽  
George P. Slater ◽  
Larry C. Fowke ◽  
Mohammed Saleem ◽  
Adrian J. Cutler
Keyword(s):  
Cell Wall ◽  
Cultured Cells ◽  
Leaf Tissue ◽  
Cell Wall Regeneration ◽  
Suspension Cultured Cells ◽  
Wall Regeneration

scholarly journals Quantitative confocal imaging method for analyzing cellulose dynamics during cell wall regeneration in Arabidopsis mesophyll protoplasts

Plant Direct ◽  
2017 ◽  
Vol 1 (6) ◽  
pp. e00021 ◽  
Author(s):  
Hiroaki Kuki ◽  
Takumi Higaki ◽  
Ryusuke Yokoyama ◽  
Takeshi Kuroha ◽  
Naoki Shinohara ◽  
...  
Keyword(s):  
Cell Wall ◽  
Confocal Imaging ◽  
Imaging Method ◽  
Cell Wall Regeneration ◽  
Mesophyll Protoplasts ◽  
Wall Regeneration

Cell Wall Regeneration and Protoplast Reversion

2020 ◽  
pp. 115-133
Author(s):  
Oldřich Nečas ◽  
Augustin Svoboda
Keyword(s):  
Cell Wall ◽  
Cell Wall Regeneration ◽  
Wall Regeneration

Ultrastructural Observations of Cell Wall Regeneration Around Isolated Tobacco Protoplasts

1974 ◽  
Vol 14 (2) ◽  
pp. 439-449
Author(s):  
J. BURGESS ◽  
E. N. FLEMING
Keyword(s):  
Electron Microscopy ◽  
Heavy Metal ◽  
Endoplasmic Reticulum ◽  
Cell Wall ◽  
Cell Wall Regeneration ◽  
Membrane Systems ◽  
Tobacco Protoplasts ◽  
Wall Formation ◽  
Wall Regeneration ◽  
Wall Growth

The process of cell wall regeneration around cultured protoplasts isolated from tobacco mesophyll has been examined by electron microscopy. The initially formed wall contains 2 components which stain with conventional heavy metal stains. The first consists of un-branched fibres, at first oriented at right angles to the plasmalemma surface. As wall growth proceeds the fibres lengthen and assume an orientation parallel to the plasmalemma. It seems probable that this component is cellulose. The second component of the wall is more amorphous and more densely stained. It is most frequently seen in situations where leaching of materials into the medium would be expected to be minimal. The endoplasmic reticulum and the plasmalemma are the only membrane systems which appear to contribute towards wall formation. No pattern of structure has been detected to explain the orientation or method of synthesis of the microfibrillar part of the wall.

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