Congress on cell and tissue culture 1994 meeting of the tissue culture association June 4–7, 1994

Abstract Background A frequent problem associated with the tissue culture of Compositae species such as chicory (Cichorium intybus L.) and lettuce (Lactuca sativa L.) is the premature bolting to in vitro flowering of regenerated plants. Plants exhibiting such phase changes have poor survival and poor seed set upon transfer from tissue culture to greenhouse conditions. This can result in the loss of valuable plant lines following applications of cell and tissue culture for genetic manipulation. Results This study demonstrates that chicory and lettuce plants exhibiting stable in vitro flowering can be rejuvenated by a further cycle of adventitious shoot regeneration from cauline leaves. The resulting rejuvenated plants exhibit substantially improved performance following transfer to greenhouse conditions, with increased frequency of plant survival, a doubling of the frequency of plants that flowered, and substantially increased seed production. Conclusion As soon as in vitro flowering is observed in unique highly-valued chicory and lettuce lines, a further cycle of adventitious shoot regeneration from cauline leaves should be implemented to induce rejuvenation. This re-establishes a juvenile phase accompanied by in vitro rosette formation, resulting in substantially improved survival, flowering and seed set in a greenhouse, thereby ensuring the recovery of future generations from lines genetically manipulated in cell and tissue culture.

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Cell and Tissue Culture

The Medical Journal of Australia ◽

10.5694/j.1326-5377.1960.tb87023.x ◽

1960 ◽

Vol 2 (3) ◽

pp. 103-103

Keyword(s):

Tissue Culture ◽

Cell And Tissue Culture

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Patterns of pigmentation in experimentally produced mouse chimaerae

Development ◽

10.1242/dev.12.4.575 ◽

1964 ◽

Vol 12 (4) ◽

pp. 575-585

Author(s):

Andrzej K. Tarkowski

Keyword(s):

Tissue Culture ◽

Annual Meeting ◽

Histological Analysis ◽

Present Report ◽

General Description ◽

Tissue Culture Association ◽

Culture In Vitro ◽

Pigment Synthesis

A General description of the development of mouse chimaerae and an account of the techniques for their production were given in previous reports (Tarkowski, 1961, 1963). The chimaeric character of the embryos and young obtained was tentatively claimed in the first of these publications because (1) the actual union of two eggs into one blastocyst was seen in culture in vitro, (2) of the occurrence of intersexes, (3) pigment synthesis of the types of the dark component occurred in the majority of individuals developed from pairs of eggs differing genetically in factors for pigmentation. The last criterion was met only by macroscopic search for pigment in the eyes. The present report gives a more detailed description of the distribution of pigment forming cells in these animals, based on histological analysis. Some remarks on the validity and applicability of such a criterion for estimating the degree of chimaerism were made at the 13th Annual Meeting of the Tissue Culture Association (Tarkowski 1963).

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