Constitutive over-expression of transforming growth factor-alpha in rat liver epithelial cells leads to increased cell cycling without transformation

1994 ◽  
Vol 30 (9) ◽  
pp. 615-621 ◽  
Author(s):  
Thomas B. Tan ◽  
Pamela A. Marino ◽  
Raji Padmanabhan ◽  
Lori L. Hampton ◽  
Joan M. Hanley-Hyde ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Rat Liver ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Alpha ◽  
Over Expression ◽  
Liver Epithelial Cells ◽  
Factor Alpha ◽  
Cell Cycling ◽  
Rat Liver Epithelial Cells

The Presence and Role of Transmembrane Transforming Growth Factor-α in Cultures of Rat Liver Epithelial Cells

1995 ◽  
Vol 218 (2) ◽  
pp. 573-576 ◽  
Author(s):  
Christine Heberden ◽  
Thierry Mercier ◽  
Isabelle Gaillard-Sanchez ◽  
Brigitte Huet ◽  
Paule Martel
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Rat Liver ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Α ◽  
Liver Epithelial Cells ◽  
Rat Liver Epithelial Cells ◽  
Factor Α

scholarly journals Immunohistochemical Localization of Transforming Growth Factor Alpha in Regenerating Rat Liver

2002 ◽  
Vol 64 (11) ◽  
pp. 1045-1048 ◽  
Author(s):  
Miyoko OKADA ◽  
Tetsuya SAKAIRI ◽  
Manami KUSAKABE ◽  
Kazuhiro GOTO ◽  
Takayuki TSUCHIYA ◽  
...  
Keyword(s):  
Growth Factor ◽  
Rat Liver ◽  
Transforming Growth Factor ◽  
Immunohistochemical Localization ◽  
Transforming Growth Factor Alpha ◽  
Regenerating Rat Liver ◽  
Factor Alpha

Effects of epidermal growth factor and transforming growth factor alpha on the mouse trophoblast outgrowth in vitro

1995 ◽  
Vol 133 (6) ◽  
pp. 741-746 ◽  
Author(s):  
Toshifumi Machida ◽  
Michiyoshi Taga ◽  
Hiroshi Minaguchi
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epithelial Cells ◽  
Conditioned Medium ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Alpha ◽  
Epidermal Growth ◽  
Factor Alpha ◽  
Endometrial Epithelial Cells

Machida T, Taga M, Minaguchi H. Effects of epidermal growth factor and transforming growth factor alpha on the mouse trophoblast outgrowth in vitro. Eur J Endocrinol 1995;133:741–6. ISSN 0804–4643 In order to analyze the involvement of growth factors in the implantation mechanism, we examined the direct effects of epidermal growth factor (EGF) and transforming growth factor alpha (TGF-α) on trophoblast outgrowth of the mouse blastocyst in vitro. ICR mouse blastocysts were cultured for 4 days on a culture plate in medium containing EGF or TGF-α or conditioned medium obtained from cultured endometrial epithelial cells. Blastocysts were also co-cultured with endometrial epithelial cells. The trophoblast outgrowth of these cultured blastocysts was observed daily and the percentage of outgrowing embryos was calculated and analyzed statistically by the chi-squared test. Analysis for the specific binding of 125I-EGF in outgrown trophoblasts was carried out by autoradiography. The coculture (days 3 and 4) and the presence of EGF (10 ng/ml, day 4), TGF-α (1 ng/ml, day 3; 10 ng/ml, days 2 and 3; 50 ng/ml, days 2–4) or conditioned medium (days 3 and 4) significantly stimulated the rate of trophoblast outgrowth. Preincubation of the conditioned medium with monoclonal anti-EGF or anti-TGF-α antibody suppressed the stimulatory effect of the conditioned medium on trophoblast outgrowth. The specific 125I-EGF binding in outgrown trophoblasts was demonstrated by autoradiography. These results suggest that EGF and TGF-α play an important role in the implantation process by directly stimulating trophoblast development. Michiyoshi Taga, Department of Obstetrics and Gynecology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236, Japan

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