Isolation of a morphologically and functionally distinct smooth muscle cell type from the intimal aspect of the normal rat aorta. Evidence for smooth muscle cell heterogeneity

1994 ◽  
Vol 30 (9) ◽  
pp. 589-595 ◽  
Author(s):  
Sergio Villaschi ◽  
Roberto F. Nicosia ◽  
Marion R. Smith
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Rat Aorta ◽  
Cell Heterogeneity ◽  
Cell Type ◽  
Muscle Cell Type ◽  
Normal Rat

scholarly journals Interstitial cells from rat middle cerebral artery belong to smooth muscle cell type

2009 ◽  
Vol 13 (11-12) ◽  
pp. 4532-4539 ◽  
Author(s):  
Maksym I. Harhun ◽  
Kinga Szewczyk ◽  
Holger Laux ◽  
Sally A. Prestwich ◽  
Dmitri V. Gordienko ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Middle Cerebral Artery ◽  
Muscle Cell ◽  
Cerebral Artery ◽  
Interstitial Cells ◽  
Cell Type ◽  
Muscle Cell Type

scholarly journals Smooth Muscle Cell Heterogeneity in Pulmonary and Systemic Vessels

1997 ◽  
Vol 17 (7) ◽  
pp. 1203-1209 ◽  
Author(s):  
Maria G. Frid ◽  
Edward C. Dempsey ◽  
Anthony G. Durmowicz ◽  
Kurt R. Stenmark
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Cell Heterogeneity

Isolation of hydroxylysyl pyridinoline,a mature collagen crosslink from neonatal rat aorta smooth muscle cell cultures

Matrix ◽  
1992 ◽  
Vol 12 (5) ◽  
pp. 381-387 ◽  
Author(s):  
Phillip J. Stone ◽  
Julianne Bryan-Rhadfi ◽  
Heather A. Shaw ◽  
Carl Franzblau
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Cell Cultures ◽  
Rat Aorta ◽  
Neonatal Rat ◽  
Smooth Muscle Cell Cultures

scholarly journals Implication of molecular vascular smooth muscle cell heterogeneity among arterial beds in arterial calcification

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0191976 ◽  
Author(s):  
Olivier Espitia ◽  
Mathias Chatelais ◽  
Marja Steenman ◽  
Céline Charrier ◽  
Blandine Maurel ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Vascular Smooth Muscle Cell ◽  
Arterial Calcification ◽  
Cell Heterogeneity

Abstract 172: Vascular Smooth Muscle Cell Activation is Regulated by miR-25 Induced miR-9 Suppression of Nox4 and Myocardin

2017 ◽  
Vol 121 (suppl_1) ◽  
Author(s):  
Brandon M Schickling ◽  
Maysam Takapoo ◽  
Eric J Devor ◽  
Francis J Miller
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Protein Expression ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Vascular Smooth Muscle Cell ◽  
Cell Activation ◽  
Rat Aorta ◽  
Neointimal Formation ◽  
Protein Levels

Vascular smooth muscle cell (SMC) de-differentiation with subsequent migration and proliferation into the subendothelial space is central to the progression of cardiovascular diseases. The Nox4 NADPH oxidase (Nox4) is implicated in maintaining the differentiated phenotype of SMC in part through myocardin, a master regulator of SMC gene expression. However, this process is poorly understood. We hypothesized that microRNAs (miR)-mediate changes in Nox4 expression and regulate SMC differentiation. Treatment of human SMCs with a miR-9 or miR-25 mimic silenced Nox4 mRNA through binding to the Nox4 3’UTR. However, only miR-25 was sufficient to downregulate Nox4 protein levels. We found that miR-25 induced the expression of miR-9 through a novel mechanism involving demethylation of the miR-9 promoter by Tet methylcytosine dioxygenase 2 (TET2). Inhibition of miR-9 induction by miR-25 with a miR-9 inhibitor restored Nox4 protein expression to basal levels. Furthermore, the miR-25-mediated decrease in Nox4 protein was ameliorated by inhibiting the proteasome with MG132. These data suggest a novel mechanism wherein miR-9 and miR-25 regulate Nox4 through both translational suppression and proteosomal degradation. Overexpression of miR-9 or miR-25 in human SMCs (1) suppressed myocardin mRNA and protein expression; (2) decreased expression of multiple SMC differentiation genes; and (3) was sufficient to induce cell migration. Thrombin and tumor necrosis factor increased the expression of miR-9 and miR-25 in human SMCs and inhibition of miR-9 prevented thrombin-mediated decrease in myocardin and SMC migration. Mir-9 and miR-25 levels were increased in SMCs derived from balloon injured rat aorta as compared to medial SMCs and in murine carotid artery ten days post carotid injury. A miR-9 inhibitor decreased neointimal formation by more than 50% in following partial carotid ligation in mice. These findings identify miR-9/Nox4 as a novel regulatory pathway of SMC differentiation and a potential therapeutic target in vascular disease.

Smooth Muscle Cell Heterogeneity

CHEST Journal ◽  
1998 ◽  
Vol 114 (1) ◽  
pp. 82S-90S ◽  
Author(s):  
Kurt R. Stenmark ◽  
Maria G. Frid
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Cell Heterogeneity

scholarly journals The effect of losartan on angiotensin II-induced cell proliferation in a rat aorta smooth muscle cell line

2012 ◽  
Vol 55 (2) ◽  
pp. 263-268 ◽  
Author(s):  
Natália Tambelline ◽  
Karen Oliveira ◽  
Luiz Renato Olchanheski Junior ◽  
Regina Sordi ◽  
Michel Fleith Otuki ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Smooth Muscle Cell ◽  
Cell Line ◽  
Muscle Cell ◽  
Rat Aorta ◽  
Muscle Cell Line
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