Radial histophysiologic gradient culture chamber: Rationale and preparation

1991 ◽  
Vol 27 (10) ◽  
pp. 786-790 ◽  
Author(s):  
Joseph Leighton
2011 ◽  
Vol 6 (1) ◽  
pp. 505 ◽  
Author(s):  
Yvonne Kohl ◽  
Gertie J Oostingh ◽  
Adam Sossalla ◽  
Albert Duschl ◽  
Hagen von Briesen ◽  
...  

1958 ◽  
Vol 4 (6) ◽  
pp. 761-764 ◽  
Author(s):  
George G. Rose ◽  
C. M. Pomerat ◽  
T. O. Shindler ◽  
J. B. Trunnell

A new technique for the cultivation of living tissues in the multipurpose culture chamber is described. This procedure employs strips of cellophane as the agent for anchoring tissue explants to the coverslip walls of the chamber and disposes of the time-honored plasma-clot technique. The primary advance embodied in this procedure lies in the fact that cells emigrating from so-cultured explants manifest themselves in a highly differentiated manner comparable to the cells of origin, whereas the outgrowth from the same types of tissue in plasma clots results in a more undifferentiated type of growth. Comparisons of outgrowths from embryonic thyroid, bone, and muscle (chicken) are photographically documented, and attention is called to certain cytochemical methods which further corroborate the differentiated quality obtained with the cellophane-strip technique.


Science ◽  
1928 ◽  
Vol 68 (1771) ◽  
pp. 571-572
Author(s):  
C. E. Hadley
Keyword(s):  

Lab on a Chip ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 2911-2926
Author(s):  
Marius Busche ◽  
Olena Tomilova ◽  
Julia Schütte ◽  
Simon Werner ◽  
Meike Beer ◽  
...  

HepaChip-MP: a 24-culture-chamber, automated microfluidic in vitro model of the liver sinusoid in multiwellplate format.


1963 ◽  
Vol 17 (2) ◽  
pp. 289-297 ◽  
Author(s):  
Murray D. Rosenberg

Observations have been made on the response, in vitro, of cultured and freshly dissociated cells to mechanical deformation. Large numbers of individual cells were studied by means of a special culture chamber bounded by two parallel glass coverslips whose spacing could be reduced from 140 to 2 microns in steps of roughly 0.5 micron. The degree of deformation required for herniation of the cell surface was measured. These measurements lead to the definition of a statistical index characteristic of the extensibility of cell surfaces. This index has been shown to be distinctive for several types of cells; to alter with certain stages of embryonic development; and to be stable with respect to the culturing of cells and certain alterations in the method of cell culture.


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